SCOPUS 2015-2019

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Postcolonial theoretical proposals in thai international relations
(Taylor and Francis, 2018) Chyatat Supachalasai
Two decades after postcolonialism became a discipline in universities in the West, it entered Thailand in the early 2000s (Nopphorn 2001; Thanes 2004) with some casting doubt over its applicability to the history of Siam/Thailand. On this view, postcolonial thought is not relevant to Thailand, a country that was not officially a Western colony but rather a semi-colony. However, some scholars in political science, International Relations (IR), cultural studies and history welcomed the implications of postcolonial thought because they perceived it as offering alternative reflections on domestic society and beyond. An example is Chairat Charoensin-o-larn who introduces postcolonial thought in order to advance a research methodology based on new inquiries that depart from the state-centric model (Chairat 2001). Chairat also devises an anti-colonial viewpoint to safeguard developing countries against the discourse of modernisation, which, for him, is another Trojan horse of the West (Chairat 2000). However, Chairat as well as other scholars in Thailand tend to focus on reading and disseminating contemporary Western theories to the academic community. Though some of them discuss Western theories at length in their works, they act simply as mediators between Western and Thai knowledge (Pinij 1971; Surak 1981, 2011; Kirathi 2001, 2002, 2012; Teerayuth 2003; Nelson 2005; Chairat 2011, 2012, 2015; Veerayuth 2013). In other words, they are only active in importing Western knowledge. They are less active in initiating their own theoretical proposals. As postcolonial thought becomes more recognised, it merges with other Western theories circulating in the Thai academic community. � 2019 selection and editorial matter, Chanintira na Thalang, Soravis Jayanama and Jittipat Poonkham; individual chapters, the contributors.
Nonlocal boundary value problems for second-order nonlinear Hahn integro-difference equations with integral boundary conditions
(Springer Verlag, 2017) Umaphon Sriphanomwan; Jessada Tariboon; Nichaphat Patanarapeelert; Sotiris K Ntouyas; Thanin Sitthiwirattham; J. Tariboon; Nonlinear Dynamic Analysis Research Center, Department of Mathematics, Faculty of Applied Science, King Mongkut�s University of Technology North Bangkok, Bangkok, 10800, Thailand; email: jessada.t@sci.kmutnb.ac.th
In this paper, we study a boundary value problem for second-order nonlinear Hahn integro-difference equations with nonlocal integral boundary conditions. Our problem contains two Hahn difference operators and a Hahn integral. The existence and uniqueness of solutions is obtained by using the Banach fixed point theorem, and the existence of at least one solution is established by using the Leray-Schauder nonlinear alternative and Krasnoselskii�s fixed point theorem. Illustrative examples are also presented to show the applicability of our results. � 2017, The Author(s).
The strategic deployment of the Airbus A350-900xWB aircraft in a full-service network carrier route network: The case of Singapore airlines
(MDPI Multidisciplinary Digital Publishing Institute, 2018) Glenn Baxter; Panarat Srisaeng; P. Srisaeng; School of Tourism and Hospitality Management, Suan Dusit University, Hua Hin, Prachaup Khiri Khan, 77110, Thailand; email: panarat_sri@dusit.ac.th
In the global airline industry, an airline�s fleet routing affects its profitability, level of service and its competitive position. Using a qualitative research approach, this paper examines Singapore Airlines Airbus A350-900XWB fleet deployment and route network development for the period 2016 to 2018. The qualitative data was examined using document analysis. The study found that Singapore Airlines has deployed the Airbus A350-900XWB aircraft on new air routes from Singapore to Cape Town via Johannesburg, D�sseldorf and Stockholm via Moscow and return. The Airbus A350-900XWB aircraft are also replacing older, less efficient aircraft as part of the company�s fleet modernization strategy. Singapore Airlines is also acquiring the new ultra-long-range variant of the Airbus A350-900XWB for use on its proposed new non-stop services from Singapore to Los Angeles and Newark Liberty Airport in New Jersey, USA. The longest flight stage length is the Singapore to San Francisco route which is 7339 nautical miles (13,594 km) in length. The shortest stage length is between Singapore and Kuala Lumpur (160 nautical miles or 297 km). The new non-stop services from Singapore to Los Angeles and New York City will be the longest non-stop services operated by Singapore Airlines. The flight stage lengths between Singapore and Los Angeles and Singapore and Newark Liberty Airport are 7621 nautical miles (14,114 km) and 8285 nautical miles (15,344 km), respectively. The greatest number of available seat kilometers (ASKs) are generated on Singapore Airlines Airbus A350-900 XWB service from Singapore to San Francisco (3.57 million ASKs). The smallest number of ASKs produced are on the short-haul service from Singapore to Kuala Lumpur (75,141 ASKs). � 2018 by the authors
Enhancement of poly(L-lactide)-degrading enzyme production by Laceyella sacchari LP175 using agricultural crops as substrates and its degradation of poly(L-lactide) polymer
(Elsevier Ltd, 2017) Thanasak Lomthong; Srisuda Hanphakphoom; Prachumporn Kongsaeree; Nantana Srisuk; Marie Guicherd; Gianluca Cioci; Sophie Duquesne; Alain Marty; Vichien Kitpreechavanich; V. Kitpreechavanich; Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand; email: fsciwck@ku.ac.th
Optimization of the medium for poly(L-lactide) (PLLA)-degrading enzyme production in shake flask experiments was investigated using agricultural products as substrates. Cassava chips and soybean meal were the best carbon and nitrogen sources, respectively, as determined by the one-factor-at-a-time method. Enzyme production was significantly enhanced by the addition of phosphates, but was suppressed by the addition of ammonium salt. The maximum enzyme production, 65.5 U/mL, was obtained from the optimized medium consisting of 4.64 g/L cassava chips, 1.53 g/L soybean meal and 0.31 g/L PLLA powder using central composite design in the basal medium. The optimal physical factors in 3 L airlift fermenter were 50 �C, pH 7.0 and 0.5 vvm aeration rate for 18 h, yielded 94.4 U/mL. The crude enzyme was shown to be able to hydrolyze PLLA powder (91%) at 50 �C in 72 h which showed high efficiency for recycling of PLLA polymer and reducing the global environmental problem. � 2017 Elsevier Ltd
Nonlocal q-symmetric integral boundary value problem for sequential q-symmetric integrodifference equations
(MDPI AG, 2018) Rujira Ouncharoen; Nichaphat Patanarapeelert; Thanin Sitthiwirattham; N. Patanarapeelert; Department of Mathematics, Faculty of Applied Science, King Mongkut's University of Technology North Bangkok, Bangkok, 10800, Thailand; email: nichaphat.p@sci.kmutnb.ac.th
In this paper, we prove the sufficient conditions for the existence results of a solution of a nonlocal q-symmetric integral boundary value problem for a sequential q-symmetric integrodifference equation by using the Banach's contraction mapping principle and Krasnoselskii's fixed point theorem. Some examples are also presented to illustrate our results. � 2018 by the authors.
Three-point fractional h-sum boundary value problems for sequential caputo fractional h-sum-difference equations
(University of Nis, 2017) Jarunee Soontharanon; Jiraporn Reunsumrit; Thanin Sitthiwirattham
In this article, we study an existence and uniqueness results for a sequential nonlinear Caputo fractional h-sum-difference equation with three-point fractional h-sum boundary conditions, by using the Banach contraction principle and the Schauder�s fixed point theorem. Our problem contains different orders in three fractional difference operators and three fractional sums. Finally, we provide an example to displays the importance of these results. � 2017, University of Nis. All rights reserved.
An assessment of sustainable airport water management: The case of Osaka�s Kansai international airport
(MDPI Multidisciplinary Digital Publishing Institute, 2018) Glenn Baxter; Panarat Srisaeng; Graham Wild; G. Wild; School of Engineering, RMIT University, Melbourne, 3000, Australia; email: graham.wild@rmit.edu.au
Airports are an essential infrastructure to facilitate aviation. The substantial growth of aviation has led to a significant increase in water usage by airports. Airports also generate large volumes of wastewater that may include contaminants. Hence, understanding sustainable water management practices is essential in the aviation industry. In this study, an exploratory research design was utilized in the examination of the sustainable water management strategies and systems at Kansai International Airport from 2002 to 2016. The qualitative data were examined using document analysis as part of a case study. The quantitative data were analyzed using regression analysis as part of a longitudinal study. The airport has been able to reduce the total water consumption, water consumption per passenger, and water consumption per aircraft movement, even with increased traffic in recent years. The airport sources water from the municipal authorities and reclaims water for non-potable water uses. The airport conducts regular water quality tests which measure the Chemical oxygen demand, total nitrogen, and total phosphates. The airport�s onsite wastewater processing centre processes all wastewaters, which discharges non-reclaimed water into Osaka Bay. With a decrease in water consumption, there has similarly been a decrease in the need to treat wastewater, while the reclaimed water ratio has increased over the period of the study. � 2018 by the authors.
Bioactive coconut protein concentrate films incorporated with antioxidant extract of mature coconut water
(Elsevier B.V., 2018) Pattrathip Rodsamran; Rungsinee Sothornvit; R. Sothornvit; Department of Food Engineering, Faculty of Engineering at Kamphaengsaen, Kasetsart University, Kamphaengsaen Campus, Nakhonpathom, 73140, Thailand; email: fengrns@ku.ac.th
Coconut is a very important fruit worldwide. Coconut milk cake and mature coconut water (CW) are the by-products from coconut processing. Therefore, it is of interest to exploit coconut milk cake and CW as a source of protein and antioxidant, respectively. In particular, antioxidant compounds from CW were extracted using a solvent (acetone and diethyl ether) and the evaporation compared with coconut water concentrate (CWC) which was extracted using evaporation. CWC had a 4-times higher total phenolic content than that of CW. Even though the solvent extract had a higher total phenolic content than the evaporation-only extract, the antioxidant capacity of the solvent extract of CWC and the evaporation-only (CWC) was not significantly different. Moreover, using CW as a solvent also reduced the amount of glycerol needed to form a satisfactory coconut protein film. The use of CW as a solvent and the incorporation of CWC significantly increased the film solubility and water vapor permeability but improved the film mechanical properties. The total phenolic content and antioxidant capacity increased in protein films incorporated with CWC and using CW as a solvent. Fourier transform infrared spectroscopy showed interactions among the antioxidant compounds from CW, CWC and protein. The formation of a polyphenol-protein complex doubled the film elongation. Thus, the coconut protein film produced using CW as a direct solvent and incorporated with CWC can provide an environmentally friendly active packaging material for agricultural and food product applications. � 2017 Elsevier Ltd
Factors that influence stay intention of Thai international students following completion of degrees in China
(Springer Netherlands, 2017) Yi Lin; Worapinya Kingminghae; Y. Lin; Pridi Banomyong International College, Thammasat University, Bangkok, 2 Prachan Road, Pranakorn, Thailand; email: slcf@163.com
Based on data recently collected in China, this study examined factors influencing Thai international students� stay/leave intentions after completion of degrees in Chinese universities. Paying attention to the concrete situations and lived experiences of international students in a more integrated Asia-Pacific region, we found that marriage/romantic relationships clearly pulled international students to their partners across borders. Business or law majors and those from business-owning families were found to be inclined to stay, possibly for economic/career reasons. Regarding acculturation attitudes, the study found that the more one attached to Thai culture or way of life, the more likely he or she planned on leaving. The reverse is also true, as pro-China attitude had a positive, albeit diminishing, effect on planning to stay. The effect of acculturation attitudes was further found to be conditional, as these personal rationales can be suppressed by family business interests. � 2016, Education Research Institute, Seoul National University, Seoul, Korea.
On nonlocal Dirichlet boundary value problem for sequential Caputo fractional Hahn integrodifference equations
(Springer International Publishing, 2018) Nichaphat Patanarapeelert; Tanapat Brikshavana; Thanin Sitthiwirattham; T. Sitthiwirattham; Mathematics Department, Faculty of Science and Technology, Suan Dusit University, Bangkok, Thailand; email: thanin_sit@dusit.ac.th
The aim of this work is to study a nonlocal Dirichlet boundary value problem for sequential Caputo fractional Hahn integrodifference equation. The problem contains two fractional Hahn difference operators and a fractional Hahn integral with different numbers of order. We use the Banach fixed point theorem to prove the existence and uniqueness of the solution. In particular, the existence of at least one solution is presented by using the Schauder fixed point theorem. � 2018, The Author(s).
Carboxymethyl cellulose from renewable rice stubble incorporated with Thai rice grass extract as a bioactive packaging film for green tea
(Blackwell Publishing Ltd, 2018) Pattrathip Rodsamran; Rungsinee Sothornvit; R. Sothornvit; Faculty of Engineering at Kamphaengsaen, Department of Food Engineering, Kasetsart University, Kamphaengsaen Campus, Nakhonpathom, Thailand; email: fengrns@ku.ac.th
The combination of commercial carboxymethyl cellulose (CMC) and CMC from rice stubble was used to form CMC-film. Additionally, CMC-film incorporated with rice grass extract microencapsulated powder (CMC-MP-film) as bioactive packaging was investigated on the quality of green tea during storage compared with commercial high density polyethylene (HDPE) film. The CMC-MP-film effectively prevented dried green tea from lipid oxidation. Moreover, the total phenolic content (TPC) of green tea packed in CMC-MP-film was equivalent to that packed in HDPE, having the highest acceptability score from the sensory evaluation. Based on the change in the TPC of the green tea drink, the estimated shelf life of green tea leaves at 25�C in HDPE, CMC-MP-film and CMC-film was 110, 110 and 91days, respectively. Thus, bioactive CMC film provides an effective alternative to synthetic film to maintain the quality and extend the shelf life of dry and oily food products. Practical applications: Rice stubble is an agricultural waste but still contains the high amount of cellulose which can be produced carboxymethyl cellulose (CMC) and used in combination of commercial CMC as biopolymer film. Moreover, rice grass microencapsulated powder was incorporated with CMC-film (CMC-MP-film) as bioactive packaging to maintain the qualities of green tea compared with commercial plastic (high density polyethylene, HDPE). The CMC-MP-film prevented effectively dried green tea from lipid oxidation. Moreover, the total phenolic content (TPC) of green tea packed in CMC-MP-film was equivalent to that packed in HDPE with similar shelf life. Therefore, bioactive CMC film provides an effective alternative to synthetic film to maintain the quality and extend the shelf life of dry and oily food products. Moreover, the CMC from rice stubble and rice grass extract, the agricultural waste from rice field, will add value as a potential renewable bioactive packaging material for the food industry. � 2018 Wiley Periodicals, Inc.
Metadiscourse Markers Analysis Used in Communication Research Articles
(Research and Development Institute Suan Dusit University, 2019) Sompoet Panawas; S. Panawas; Faculty of Humanities and Social Sciences, Suan Dusit University, Bangkok, 10700, Thailand; email: spanawas@yahoo.com
This research aims to analyze the metadiscourse markers used in communication research articles in terms of studying: (1) the frequency of using metadiscourse markers, (2) the differences between the metadiscourse markers used in the introduction part and the discussion part of the communication research articles and (3) the correlation of 14 metadiscourse markers in 4 categories of metadiscourse markers; self-mention words, attitudinal markers, boosters and hedge. The language data corpus used is 20 communication research articles and only the introduction parts and discussion parts of each research article were analyzed. The instruments used are (1) AntConc 3.2 computer program for research articles random and (2) PASW 18.0 computer program for statistical analysis. The findings revealed that: (1) the three most frequently used markers are attitudinal markers; adjective, booster; verb and hedge; modal verb, (2) there are 9 metadiscourse markers used in the introduction parts and the discussion parts that are significantly different and the other 5 markers are not significantly different and (3) there are 13 metadiscourse markers used in the communication research articles that show correlation and 1 marker that shows no correlation. � 2019, Research and Development Institute Suan Dusit University. All rights reserved.
Existence results of a coupled system of Caputo fractional Hahn difference equations with nonlocal fractional Hahn integral boundary value conditions
(MDPI AG, 2018) Thongchai Dumrongpokaphan; Nichaphat Patanarapeelert; Thanin Sitthiwirattham; N. Patanarapeelert; Department of Mathematics, Faculty of Applied Science, King Mongkut's University of Technology North Bangkok, Bangkok, 10800, Thailand; email: nichaphat.p@sci.kmutnb.ac.th
In this article, we propose a coupled system of Caputo fractional Hahn difference equations with nonlocal fractional Hahn integral boundary conditions. The existence and uniqueness result of solution for the problem is studied by using the Banach's fixed point theorem. Furthermore, the existence of at least one solution is presented by using the Schauder fixed point theorem. � 2018 by the authors.
Microwave heating extraction of pectin from lime peel: Characterization and properties compared with the conventional heating method
(Elsevier Ltd, 2019) Pattrathip Rodsamran; Rungsinee Sothornvit; R. Sothornvit; Department of Food Engineering, Faculty of Engineering at Kamphaengsaen, Kasetsart University, Kamphaengsaen Campus, 73140, Thailand; email: fengrns@ku.ac.th
Microwave and conventional heating methods were used to extract pectin from lime peel waste using different acid extractants (hydrochloric or citric acid) and peel-to-extractant ratios (1:20 or 1:40). Hydrochloric acid as the extractant resulted in a higher yield of pectin with both methods. The methoxyl content and galacturonic acid content of lime peel pectin were in the range 8.74�10.51% and 79.29�95.93%, respectively. The intense band around 1730 cm_1 corresponded to methyl esterified uronic carboxyl groups and confirmed the higher equivalent weight and degree of esterification for the microwave-extracted pectin than that from conventional extraction. Lime peel pectin could be classified as high methoxyl pectin having a rapid-set gel formation. The viscosity and viscoelastic properties of the pectin solution from both heating methods enhanced with increasing solid concentration. Hence, microwave heating can be a short processing time for pectin extraction from lime peel waste with suitable pectin properties. � 2018 Elsevier Ltd
Effect of bagasse and bagasse ash levels on properties of pottery products
(Elsevier Ltd, 2018) Sutas Janbuala; Mana Eambua; Arpapan Satayavibul; Watcharakhon Nethan; S. Janbuala; Faculty of Science, Suan Dusit University, Bangkok, 10300, Thailand; email: sutas_jan@dusit.ac.th
The objective of this research was to study the effect of bagasse and bagasse ash on the properties of pottery products. In the experiments, we varied the composition of clay by adding 2%, 4%, 6%, and 8% by weight of bagasse or by adding 5%, 10%, 15%, and 20% by weight of bagasse ash and maintained the temperature of the furnace at 900 �C. The results indicate that the best composition is obtained by adding 10% by weight of bagasse ash, which yielded pottery products with improved strength (20.10 MPa), density (1.41 g/cm3), water absorption (13.91%), and porosity (25.73%). In comparison, the composition of clay with 4% by weight of bagasse yielded strength, density, water absorption, and porosity of 10.12 MPa, 1.53 g/cm3, 19.95%, and 30.17%, respectively. � 2018 The Authors
Microencapsulation of Thai rice grass (O. Sativa cv. Khao Dawk Mali 105) extract incorporated to form bioactive carboxymethyl cellulose edible film
(Elsevier Ltd, 2018) Pattrathip Rodsamran; Rungsinee Sothornvit; R. Sothornvit; Department of Food Engineering, Faculty of Engineering at Kamphaengsaen/Center of Advanced Studies in Industrial Technology, Kasetsart University, Nakhonpathom, Kamphaengsaen Campus, 73140, Thailand; email: fengrns@ku.ac.th
Microencapsulation was investigated to enhance the stability of Thai rice grass extract. Microencapsulated powder (MP) was formed using total solid of extract solution and maltodextrin ratios of 1:4 (MP 1:4) and 1:9 (MP 1:9). The absence of an endothermic peak for both MPs confirmed all extract solutions were coated with maltodextrin. MP 1:9 had a lower total phenolic content (TPC) but was higher in antioxidant capacity than MP 1:4. Moreover, the TPC of the MPs slightly decreased (70.02�93.04%) during storage at 10, 30 and 70 �C for 30 d. Comparatively, the TPC of the extract solution significantly decreased from 100% down to 20.8%, 11.2% and 8.6% at 10, 30 and 70 �C, respectively. Therefore, MP 1:9 incorporated with blended carboxymethyl cellulose film increased the water barrier and the TPC. This film can serve as a bioactive biodegradable packaging material to reduce plastic packaging in the food industry. � 2017 Elsevier Ltd
On four-point fractional q-integrodifference boundary value problems involving separate nonlinearity and arbitrary fractional order
(Springer International Publishing, 2018) Nichaphat Patanarapeelert; Thanin Sitthiwirattham; T. Sitthiwirattham; Mathematics Department, Faculty of Science and Technology, Suan Dusit University, Bangkok, Thailand; email: thanin_sit@dusit.ac.th
In this paper, we study a sequential Caputo fractional q-integrodifference equation with fractional q-integral and Riemann�Liouville fractional q-derivative boundary value conditions. Our problem contains 2 (M+ N+ 1) different orders and six different numbers of q in derivatives and integrals. The problem contains separate nonlinear functions. To examine existence and uniqueness results of the problem, Banach�s contraction principle and the Leray�Schauder nonlinear alternative are employed. An illustrative example is also provided. � 2018, The Author(s).
An assessment of airport sustainability, part 2-Energy management at Copenhagen Airport
(MDPI AG, 2018) Glenn Baxter; Panarat Srisaeng; Graham Wild; G. Wild; School of Engineering, RMIT University, Melbourne, 3000, Australia; email: graham.wild@rmit.edu.au
Airports play a critical role in the air transport value chain. Each air transport value chain stakeholder requires energy to conduct their operations. Airports are extremely energy intensive. Greenhouse gases are a by-product from energy generation and usage. Consequently, airports are increasingly trying to sustainably manage their energy requirements as part of their environmental policies and strategies. This study used an exploratory qualitative and quantitative case study research approach to empirically examine Copenhagen Airport, Scandinavia's major air traffic hub, sustainable airport energy management practices and energy-saving initiatives. For Copenhagen Airport, the most significant environmental impact factors occurring from energy usage are the CO2 emissions arising from both the air side and land side operations. Considering this, the airport has identified many ways to manage and mitigate the environmental impact from energy consumption on both the air and land side operations. Importantly, the application of technological solutions, systems and process enhancements and collaboration with key stakeholders has contributed to the airport's success in mitigating the environmental impact from energy usage at the airport whilst at the same time achieving energy savings. � 2018 by the authors.
Development of Digital Media using Augmented Reality for HM King Prajadhipok's Interests in Arts and Culture
(Institute of Electrical and Electronics Engineers Inc., 2018) Porawat Visutsak; Fuangfar Pensiri
For Thai people, the kingship is just not the institution; Thai people respect the King like the father of the land. The good evidence to show that the kingship is revered by Thai people and admired throughout the world is the statement given by the president of the 71st session of the UN general assembly plenary meeting to pay tribute to HM King Bhumibol Adulyadej (King Rama IX), who passed away on 13 October 2016. In the past few years, to study the history of Thailand according to each reign of the dynasty, Thai people need to study the documents, the media and films from the various data sources. With the current trends of the augmented reality and digital technologies, people can study the multimodal media interactively at hands. This study aims to gather evidences and archives of HM King Prajadhipok, the 7th King of the House of Chakri (King Rama VII). The current study of HM King Prajadhipok is mostly focusing on the political conflicts and social changes during the Revolution of 1932. Documents and media about HM King Prajadhipok's interests in arts, music, paintings and films have not been yet gathered and archived successfully. The main contribution of this work has been installed in the archival King Prajadhipok study room, the office of documentation and information, Sukhothai Thammathirat Open University (STOU), Bangkok, Thailand. The virtual contents consist of photographs, documents and films concerning the HM King Prajadhipok's life during his reign and after the abdication. The archival of this work mainly focuses on the architectures, the royal's song writing, films making and paintings to promote his interests in arts and culture. � 2018 IEEE.
Characterization of an Anaerobic, Thermophilic, Alkaliphilic, High Lignocellulosic Biomass-Degrading Bacterial Community, ISHI-3, Isolated from Biocompost
(Elsevier Inc., 2018) Ayumi Shikata; Junjarus Sermsathanaswadi; Phakhinee Thianheng; Sirilak Baramee; Chakrit Tachaapaikoon; Rattiya Waeonukul; Patthra Pason; Khanok Ratanakhanokchai; Akihiko Kosugi; A. Kosugi; Biological Resources and Post-harvest Division, Japan International Research Center for Agricultural Sciences (JIRCAS), Tsukuba, 1-1 Ohwashi, 305-8686, Japan; email: akosugi@affrc.go.jp
The generation of a complex microbial consortium is a promising approach for efficient biomass decomposition. An anaerobic thermophilic alkaliphilic microbial consortium with efficient degradation ability was screened from bovine manure compost using non-pretreated milling corn stover (CS) and rice straw (RS). A stable microbial consortium ISHI-3 with high degradation ability for CS and RS was isolated by the roll tube technique. ISHI-3 comprised Herbivorax saccincola and bacteria belonging to the classes Pelotomaculum, Tepidanaerobacter, and Tepidimicrobium, as determined by DGGE of the PCR-generated 16S rRNA genes. Furthermore, metagenomics analysis using a 16S rRNA library was carried out to determine the bacterial distribution during degradation of CS and RS. H. saccincola and bacteria belonging to Pelotomaculum were relatively abundant in the beginning to middle periods of culture with CS and RS whereas bacteria belonging to Tepidanaerobacter and Tepidimicrobium gradually increased in the population during the later stages. To understand the role of non-cellulolytic bacteria in the consortium, novel strains ET1 and GL4, which were most closely related to Tepidimicrobium ferriphilum and Tepidanaerobacter acetatoxydans, were isolated from ISHI-3. Based on their carbon source usage, morphology, and phylogenetic analysis, we propose that strains ET1 and GL4 should be classified as a novel genus or species. Bacteria ET1 and GL4 can utilize different organic compounds as carbon and energy sources such as organic acids, alcohols, sugars, and amino acids, showing a preference for organic acids and alcohols rather than sugars such as glucose and cellobiose. These results indicated that ET1 and GL4 help to accelerate efficient lignocellulose degradation of H. saccincola. � 2018 Elsevier Inc.

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