Enhancement of poly(L-lactide)-degrading enzyme production by Laceyella sacchari LP175 using agricultural crops as substrates and its degradation of poly(L-lactide) polymer

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dc.contributor.authorThanasak Lomthong
dc.contributor.authorSrisuda Hanphakphoom
dc.contributor.authorPrachumporn Kongsaeree
dc.contributor.authorNantana Srisuk
dc.contributor.authorMarie Guicherd
dc.contributor.authorGianluca Cioci
dc.contributor.authorSophie Duquesne
dc.contributor.authorAlain Marty
dc.contributor.authorVichien Kitpreechavanich
dc.contributor.correspondenceV. Kitpreechavanich; Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand; email: fsciwck@ku.ac.th
dc.date.accessioned2025-03-10T07:36:32Z
dc.date.available2025-03-10T07:36:32Z
dc.date.issued2017
dc.description.abstractOptimization of the medium for poly(L-lactide) (PLLA)-degrading enzyme production in shake flask experiments was investigated using agricultural products as substrates. Cassava chips and soybean meal were the best carbon and nitrogen sources, respectively, as determined by the one-factor-at-a-time method. Enzyme production was significantly enhanced by the addition of phosphates, but was suppressed by the addition of ammonium salt. The maximum enzyme production, 65.5 U/mL, was obtained from the optimized medium consisting of 4.64 g/L cassava chips, 1.53 g/L soybean meal and 0.31 g/L PLLA powder using central composite design in the basal medium. The optimal physical factors in 3 L airlift fermenter were 50 �C, pH 7.0 and 0.5 vvm aeration rate for 18 h, yielded 94.4 U/mL. The crude enzyme was shown to be able to hydrolyze PLLA powder (91%) at 50 �C in 72 h which showed high efficiency for recycling of PLLA polymer and reducing the global environmental problem. � 2017 Elsevier Ltd
dc.identifier.citationPolymer Degradation and Stability
dc.identifier.doi10.1016/j.polymdegradstab.2017.06.017
dc.identifier.issn1413910
dc.identifier.scopus2-s2.0-85021172598
dc.identifier.urihttps://repository.dusit.ac.th//handle/123456789/4942
dc.languageEnglish
dc.publisherElsevier Ltd
dc.rights.holderScopus
dc.subjectAgricultural products
dc.subjectBiodegradation
dc.subjectLaceyella sacchari LP175
dc.subjectPoly(L-lactide)-degrading enzyme
dc.subjectSubmerged culture
dc.titleEnhancement of poly(L-lactide)-degrading enzyme production by Laceyella sacchari LP175 using agricultural crops as substrates and its degradation of poly(L-lactide) polymer
dc.typeArticle
mods.location.urlhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85021172598&doi=10.1016%2fj.polymdegradstab.2017.06.017&partnerID=40&md5=8a41a7fb9409eeaa2a367e1f12f956dd
oaire.citation.endPage73
oaire.citation.startPage64
oaire.citation.volume143
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