In vitro production of functional immune cells derived from human hematopoietic stem cells

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dc.contributor.authorWitchuda Payuhakrit
dc.contributor.authorTasanee Panichakul
dc.contributor.authorNatthawut Charoenphon
dc.contributor.authorPanus Chalermsaenyakorn
dc.contributor.authorAdithep Jaovisidha
dc.contributor.authorChokdee Wongborisuth
dc.contributor.authorRachanee Udomsangpetch
dc.date.accessioned2025-03-10T07:36:30Z
dc.date.available2025-03-10T07:36:30Z
dc.date.issued2015
dc.description.abstractHematopoietic stem cells (HSC) from cord blood are potentially high sources for transplantation due to their low immunogenicity and the presence of the multipotent cells. These cells are capable of differentiating to produce various lineages of blood cells under specific conditions. We have enriched highly purified CD34+ cells from cord blood, determined in vitro growth of the cells in culture systems in the absence (condition A) or presence of GM-CSF and G-CSF (condition B), and determined the profile of immune cells during the period of cultivation by using flow cytometry. PhytohemagglutininA (PHA) was used as a mitogen to stimulate T lymphocytes derived from hematopoietic stem cells. GM-CSF and G-CSF prolonged the survival of the growing cells and also maintained expansion of cells in blastic stage. By day 12 of cultivation, when cell numbers peaked, various types of immune cells had appeared (CD14+ cells, CD40+HLA-DR+ cells, CD3+CD56+ cells, CD19+ cells, CD3+CD4+ cells, CD3+CD8+cells and CD3-CD56+). A significantly higher percentage of monocytes (p = 0.002) were observed under culture with GM-CSF, G-CSF when compared with culture without GM-CSF, G-CSF. In addition, T lymphocytes derived from HSC responded to 50 _g/ml of PHA. This is the first report showing the complete differentiation and proliferation of immune cells derived from CD34+ HSC under in vitro culture conditions. Lymphocytes, monocytes, dendritic cells and polymorph nuclear cells derived from HSC in vitro are unique, and thus may benefit various studies such as innate immunity and pathophysiology of immune disorders. � 2015, Leibniz Research Centre for Working Environment and Human Factors. All right reserved.
dc.identifier.citationEXCLI Journal
dc.identifier.doi10.17179/excli2015-506
dc.identifier.issn16112156
dc.identifier.scopus2-s2.0-84941348635
dc.identifier.urihttps://repository.dusit.ac.th//handle/123456789/4842
dc.languageEnglish
dc.publisherLeibniz Research Centre for Working Environment and Human Factors
dc.rights.holderScopus
dc.subjectHematopoietic stem cells
dc.subjectLymphocyte maturation
dc.subjectMyelopoietic cells
dc.subjectStem cells
dc.titleIn vitro production of functional immune cells derived from human hematopoietic stem cells
dc.typeArticle
mods.location.urlhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84941348635&doi=10.17179%2fexcli2015-506&partnerID=40&md5=77eaeaba329962ee5ed922b888743661
oaire.citation.endPage1039
oaire.citation.startPage1031
oaire.citation.volume14
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