Co-production of poly(l-lactide)-degrading enzyme and raw starch-degrading enzyme by Laceyella sacchari LP175 using agricultural products as substrate, and their efficiency on biodegradation of poly(l-lactide)/thermoplastic starch blend film

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dc.contributor.authorThanasak Lomthong
dc.contributor.authorSrisuda Hanphakphoom
dc.contributor.authorRangrong Yoksan
dc.contributor.authorVichien Kitpreechavanich
dc.date.accessioned2025-03-10T07:36:30Z
dc.date.available2025-03-10T07:36:30Z
dc.date.issued2015
dc.description.abstractThe co-production of poly-(l-lactide)-degrading enzyme and raw starch-degrading enzyme by the thermophilic filamentous bacterium Laceyella sacchari LP175 in liquid medium using low-cost agricultural crops as substrates was investigated. Statistical mixture design experiments indicated that 5g of raw material - consisting of 2.35gL-1 cassava chips and 2.65gL-1 soybean meal in a suspension of 2.0gL-1 K2HPO4 and 1.0gL-1 KH2PO4 - gave the highest production of both enzymes when the culture was grown at 50�C for 24h cultivation. Addition of 1.0gL-1 of poly-(l-lactide) powder and 1.0gL-1 cassava starch to the medium increased poly-(l-lactide)-degrading enzyme and raw starch-degrading enzyme, respectively. Response surface methodology by central composite design found that the optimized concentration of 0.52gL-1 poly(l-lactide) powder and 3.34gL-1 cassava starch increased poly-(l-lactide)-degrading enzyme and raw starch-degrading enzyme activities up to 68.8UmL-1 and 86.1UmL-1, respectively. The 2% poly-(l-lactide)/thermoplastic starch (PLLA/TPS) blend (50:50) film was degraded up to 99.7% of weight loss by the crude enzyme at an initial pH of 9.0 for 4h. The high efficiency on biodegradation of poly-(l-lactide)/thermoplastic starch blend polymer by the obtained mixed enzymes from cheap and abundant agricultural products could be applied to reduce global environment from non-biodegradable materials. � 2015 Elsevier Ltd.
dc.identifier.citationInternational Biodeterioration and Biodegradation
dc.identifier.doi10.1016/j.ibiod.2015.07.011
dc.identifier.issn9648305
dc.identifier.scopus2-s2.0-84938078876
dc.identifier.urihttps://repository.dusit.ac.th//handle/123456789/4841
dc.languageEnglish
dc.publisherElsevier Ltd
dc.rights.holderScopus
dc.subjectBiodegradation
dc.subjectLaceyella sacchari LP175
dc.subjectMixture design
dc.subjectPoly(l-lactide)-degrading enzyme
dc.subjectRaw starch-degrading enzyme
dc.titleCo-production of poly(l-lactide)-degrading enzyme and raw starch-degrading enzyme by Laceyella sacchari LP175 using agricultural products as substrate, and their efficiency on biodegradation of poly(l-lactide)/thermoplastic starch blend film
dc.typeArticle
mods.location.urlhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84938078876&doi=10.1016%2fj.ibiod.2015.07.011&partnerID=40&md5=2ad77c26e5523376d33d13cea832a2ae
oaire.citation.endPage410
oaire.citation.startPage401
oaire.citation.volume104
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