The GH67 _-glucuronidase of Paenibacillus curdlanolyticus B-6 removes hexenuronic acid groups and facilitates biodegradation of the model xylooligosaccharide hexenuronosyl xylotriose

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dc.contributor.authorKrisna Septiningrum
dc.contributor.authorHiroshi Ohi
dc.contributor.authorRattiya Waeonukul
dc.contributor.authorPatthra Pason
dc.contributor.authorChakrit Tachaapaikoon
dc.contributor.authorKhanok Ratanakhanokchai
dc.contributor.authorJunjarus Sermsathanaswadi
dc.contributor.authorLan Deng
dc.contributor.authorPanida Prawitwong
dc.contributor.authorAkihiko Kosugi
dc.date.accessioned2025-03-10T07:36:30Z
dc.date.available2025-03-10T07:36:30Z
dc.date.issued2015
dc.description.abstract4-O-Methylglucuronic acid (MeGlcA) side groups attached to the xylan backbone through _-1,2 linkages are converted to hexenuronic acid (HexA) during alkaline pulping. _-Glucuronidase (EC 3.2.1.139) hydrolyzes 1,2-linked MeGlcA from xylooligosaccharides. To determine whether _-glucuronidase can also hydrolyze HexA-decorated xylooligosaccharides, a gene encoding _-glucuronidase (AguA) was cloned from Paenibacillus curdlanolyticus B-6. The purified protein degraded hexenuronosyl xylotriose (_X3), a model substrate prepared from kraft pulp. AguA released xylotriose and HexA from _X3, but the Vmax and kcat values for _X3 were lower than those for MeGlcA, indicating that HexA side groups may affect the hydrolytic activity. To explore the potential for biological bleaching, _X3 degradation was performed using intracellular extract from P. curdlanolyticus B-6. The intracellular extract, with synergistic _-glucuronidase and _-xylosidase activities, degraded _X3 to xylose and HexA. These results indicate that _-glucuronidase can be used to remove HexA from _X3 derived from pulp, reducing the need for chemical treatments in the pulping process. � 2015 Elsevier Inc.
dc.identifier.citationEnzyme and Microbial Technology
dc.identifier.doi10.1016/j.enzmictec.2015.01.006
dc.identifier.issn1410229
dc.identifier.scopus2-s2.0-84923059877
dc.identifier.urihttps://repository.dusit.ac.th//handle/123456789/4843
dc.languageEnglish
dc.publisherElsevier Inc.
dc.rights.holderScopus
dc.subjectGH67
dc.subjectHexenuronic acid
dc.subjectHexenuronosyl xylotriose
dc.subjectPaenibacillus curdlanolyticus
dc.subject_-Glucuronidase
dc.titleThe GH67 _-glucuronidase of Paenibacillus curdlanolyticus B-6 removes hexenuronic acid groups and facilitates biodegradation of the model xylooligosaccharide hexenuronosyl xylotriose
dc.typeArticle
mods.location.urlhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84923059877&doi=10.1016%2fj.enzmictec.2015.01.006&partnerID=40&md5=a55d072b32a134fb4bcb678358cb07f4
oaire.citation.endPage35
oaire.citation.startPage28
oaire.citation.volume71
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