Characterization and high-quality draft genome sequence of Herbivorax saccincola A7, an anaerobic, alkaliphilic, thermophilic, cellulolytic, and xylanolytic bacterium

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dc.contributor.authorShimpei Aikawa
dc.contributor.authorSirilak Baramee
dc.contributor.authorJunjarus Sermsathanaswadi
dc.contributor.authorPhakhinee Thianheng
dc.contributor.authorChakrit Tachaapaikoon
dc.contributor.authorAyumi Shikata
dc.contributor.authorRattiya Waeonukul
dc.contributor.authorPatthra Pason
dc.contributor.authorKhanok Ratanakhanokchai
dc.contributor.authorAkihiko Kosugi
dc.contributor.correspondenceA. Kosugi; Biological Resources and Post-Harvest Division, Japan International Research Center for Agricultural Sciences (JIRCAS), Tsukuba, 1-1 Ohwashi, 305-8686, Japan; email: akosugi@affrc.go.jp
dc.date.accessioned2025-03-10T07:36:32Z
dc.date.available2025-03-10T07:36:32Z
dc.date.issued2018
dc.description.abstractAn anaerobic, cellulolytic-xylanolytic bacterium, designated strain A7, was isolated from a cellulose-degrading bacterial community inhabiting bovine manure compost on Ishigaki Island, Japan, by enrichment culture using unpretreated corn stover as the sole carbon source. The strain was Gram-positive, non-endospore forming, non-motile, and formed orange colonies on solid medium. Strain A7 was identified as Herbivorax saccincola by DNA-DNA hybridization, and phylogenetic analysis based on 16S rRNA gene sequences showed that it was closely related to H. saccincola GGR1 (= DSM 101079T). H. saccincola A7 (= JCM 31827 = DSM 104321) had quite similar phenotypic characteristics to those of strain GGR1. However, the optimum growth of A7 was at alkaline pH (9.0) and 55 �C, compared to pH 7.0 at 60 �C for GGR1, and the fatty acid profile of A7 contained 1.7-times more C17:0 iso than GGR1. The draft genome sequence revealed that H. saccincola A7 possessed a cellulosome-like extracellular macromolecular complex, which has also been found for Clostridium thermocellum and C. clariflavum. H. saccincola A7 contained more glycoside hydrolases (GHs) belonging to GH families-11 and -2, and more diversity of xylanolytic enzymes, than C. thermocellum and C. clariflavum. H. saccincola A7 could grow on xylan because it encoded essential genes for xylose metabolism, such as a xylose transporter, xylose isomerase, xylulokinase, and ribulose-phosphate 3-epimerase, which are absent from C. thermocellum. These results indicated that H. saccincola A7 has great potential as a microorganism that can effectively degrade lignocellulosic biomass. � 2018 Elsevier GmbH
dc.identifier.citationSystematic and Applied Microbiology
dc.identifier.doi10.1016/j.syapm.2018.01.010
dc.identifier.issn7232020
dc.identifier.scopus2-s2.0-85042360404
dc.identifier.urihttps://repository.dusit.ac.th//handle/123456789/4921
dc.languageEnglish
dc.publisherElsevier GmbH
dc.rightsAll Open Access; Bronze Open Access
dc.rights.holderScopus
dc.subjectAlkaliphile
dc.subjectCellulolytic bacterium
dc.subjectCellulosome
dc.subjectGenome sequence
dc.subjectHerbivorax saccincola
dc.subjectLignocellulose
dc.titleCharacterization and high-quality draft genome sequence of Herbivorax saccincola A7, an anaerobic, alkaliphilic, thermophilic, cellulolytic, and xylanolytic bacterium
dc.typeArticle
mods.location.urlhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85042360404&doi=10.1016%2fj.syapm.2018.01.010&partnerID=40&md5=2e043356f2d211c7efb4beb4030174ff
oaire.citation.endPage269
oaire.citation.issue4
oaire.citation.startPage261
oaire.citation.volume41
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