Browsing by Author "Tasanee Panichakul"

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    Additive effect of a combination of artocarpus lakoocha and glycyrrhiza glabra extracts on tyrosinase inhibition in melanoma B16 cells
    (MDPI AG, 2020) Tasanee Panichakul; Teerapat Rodboon; Prasit Suwannalert; Chanchai Tripetch; Rittipun Rungruang; Nattaporn Boohuad; Piyawan Youdee; T. Panichakul; Department of Cosmetic Science, Faculty of Science and Technology, Suan Dusit University, Bangkok, 228-228/1-3 Sirindhorn Rd. Bangplad, 10700, Thailand; email: tasanee_pan@dusit.ac.th
    Artocarpus lakoocha (Al) and Glycyrrhiza glabra (Gg) extracts have been reported to show tyrosinase inhibitory activity and melanin pigment reduction. This is the first study to assess the combination of Al and Gg extracts in enhancing inhibition of tyrosinase and reduction of melanin pigments. Al and Gg extracted by maceration in 70% and 95% ethanol were analyzed for oxyresveratrol and glabridin using Ultra High Performance Liquid Chromatography. Extracts of Al and Gg singly and combinations of Al95 and Gg95 were tested for cytotoxicity, tyrosinase inhibitory activity, and reduction of melanin pigments in melanoma B16 cells. Al95 had higher antioxidant, tyrosinase inhibitory activity and reduced more melanin pigments in B16 cells compared to Al 70, and exhibited higher levels of oxyresveratrol. Gg95 inhibited oxidative stress and mushroom tyrosinase better than Gg70, and exhibited higher levels of glabridin. Combinations of Al95 and Gg95 at various ratios (concentration of 0.1 mg/mL) were not cytotoxic to B16 cells. Interestingly, Al95 and Gg95 combined at a ratio 9:1 reduced melanin pigment up to 53% in B16 cells. This combination of Al95 and Gg95 extracts exhibited the additive effect of reducing melanin pigments by suppressing the expression of microphthalmia-associated transcription factor (MITF), tyrosinase (TYR) and tyrosinase-related protein-2 (TRP-2) in B16 cells. The combination of Al and Gg extracts could be developed as skin care products for hyperpigmentation treatment. © 2020 by the authors.
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    Antimicrobial Effect of Deodorant Products Containing Rhinacanthus nasutus Extract for Reducing Armpit Odor
    (Research and Development Institute Suan Dusit University, 2022) Nartlada Onvimol; Rittipun Rungruang; Surapha Modsuwan; Tasanee Panichakul; T. Panichakul; Faculty of Science and Technology, Suan Dusit University, Bangkok, 10700, Thailand; email: tasanee_p@yahoo.com
    The aim of this study was to investigate the effectiveness of developing a roll-on deodorant that can reduce armpit microbiota bacteria to decrease armpit odor. The richest apocrine sweat glands in the armpit region secrete a variety of odor precursors that are transformed into volatile odoriferous substances by bacterial enzymes on the skin surface. The dominant armpits microbiota included four groups of bacteria such as Staphylococcus spp., Micrococcus spp., Corynebacterium spp., and Propionibacterium spp., and also fungi or yeasts. Two formulas of the roll-on deodorant products, RDEOF-1 and RDEOF-2 were developed. RDEOF-2 contained an ethanolic extract of Rhinacanthus nasutus (L.) Kurz leaves inhibited several microorganisms, including Gram-positive and Gram-negative bacteria. Sixteen healthy volunteers showed satisfactory assessment for RDEOF-2 that was greater than RDEOF-1. Armpit bacteria were collected by swab method and armpit odor was evaluated by ASTM method. Results showed dominant bacteria of two genera including Staphylococcus spp. and Corynebacterium spp. in all swab samples that mainly cause armpit odor. Armpit bacterial numbers before using deodorants were high in the range of 2x103 to 9x105 (CFU/mL). After applying RDEOF-1 and RDEOF-2, bacterial numbers decreased in the range of 1x103 to 8x105 and 3x102 to 4x105 (CFU/mL), respectively. Armpit bacteria were found in males more than in females. Deodorant products containing R. nasutus extract have been shown to reduce the bacteria that cause armpit odor. Therefore, the development of the deodorant product with natural plant extracts is warranted. © 2022, Research and Development Institute Suan Dusit University. All rights reserved.
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    Antimicrobial Effect of Deodorant Products Containing Rhinacanthus nasutus Extract for Reducing Armpit Odor
    (Graphicsite, 2023-09-26) Nartlada Onvimol; Rittipun Rungruang; Surapha Modsuwan; Tasanee Panichakul
    The aim of this study was to investigate the effectiveness of developing a roll-on deodorant that can reduce armpit microbiota bacteria to decrease armpit odor. The richest apocrine sweat glands in the armpit region secrete a variety of odor precursors that are transformed into volatile odoriferous substances by bacterial enzymes on the skin surface. The dominant armpits microbiota included four groups of bacteria such as Staphylococcus spp., Micrococcus spp., Corynebacterium spp., and Propionibacterium spp., and also fungi or yeasts. Two formulas of the roll-on deodorant products, RDEOF-1 and RDEOF-2 were developed. RDEOF-2 contained an ethanolic extract of Rhinacanthus nasutus (L.) Kurz leaves inhibited several microorganisms, including Gram-positive and Gram-negative bacteria. Sixteen healthy volunteers showed satisfactory assessment for RDEOF-2 that was greater than RDEOF-1. Armpit bacteria were collected by swab method and armpit odor was evaluated by ASTM method. Results showed dominant bacteria of two genera including Staphylococcus spp. and Corynebacterium spp. in all swab samples that mainly cause armpit odor. Armpit bacterial numbers before using deodorants were high in the range of 2x103 to 9x105 (CFU/mL). After applying RDEOF-1 and RDEOF-2, bacterial numbers decreased in the range of 1x103 to 8x105 and 3x102 to 4x105 (CFU/mL), respectively. Armpit bacteria were found in males more than in females. Deodorant products containing R. nasutus extract have been shown to reduce the bacteria that cause armpit odor. Therefore, the development of the deodorant product with natural plant extracts is warranted.
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    Characterization of inhibitory anti-duffy binding protein II immunity: Approach to plasmodium vivax vaccine development in Thailand
    (2012) Patchanee Chootong; Tasanee Panichakul; Chongrak Permmongkol; Samantha J. Barnes; Rachanee Udomsangpetch; John H. Adams; P. Chootong; Department of Clinical Microbiology and Applied Technology, Faculty of Medical Technology, Mahidol University, Bangkok, Thailand; email: pchooton@gmail.com
    Plasmodium vivax Duffy binding protein region II (DBPII) is an important vaccine candidate for antibody-mediated immunity against vivax malaria. A significant challenge for vaccine development of DBPII is its highly polymorphic nature that alters sensitivity to neutralizing antibody responses. Here, we aim to characterize naturally-acquired neutralizing antibodies against DBPII in individual Thai residents to give insight into P. vivax vaccine development in Thailand. Anti-DBPII IgG significantly increased in acute vivax infections compared to uninfected residents and naive controls. Antibody titers and functional anti-DBPII inhibition varied widely and there was no association between titer and inhibition activity. Most high titer plasmas had only a moderate to no functional inhibitory effect on DBP binding to erythrocytes, indicating the protective immunity against DBPII binding is strain specific. Only 5 of 54 samples were highly inhibitory against DBP erythrocyte-binding function. Previously identified target epitopes of inhibitory anti-DBPPII IgG (H1, H2 and H3) were localized to the dimer interface that forms the DARC binding pocket. Amino acid polymorphisms (monomorphic or dimorphic) in H1 and H3 protective epitopes change sensitivity of immune inhibition by alteration of neutralizing antibody recognition. The present study indicates Thai variant H1.T1 (R308S), H3.T1 (D384G) and H3.T3 (K386N) are the most important variants for a DBPII candidate vaccine needed to protect P. vivax in Thai residents. © 2012 Chootong et al.
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    Effects of extraction methods on the flavonoid and phenolic contents and anti-aging properties of Rhyncholaeliocattleya Haw Yuan Beauty extracts
    (Science Society of Thailand under Royal Patronage, 2021) Rittipun Rungruang; Tasanee Panichakul; Wittawat Rattanathavorn; Nattapon Kaisangsri; Orapin Kerdchoechuen; Natta Laohakunjit; Louis Kuoping Chao; R. Rungruang; Department of Cosmetic Science, Faculty of Science and Technology, Suan Dusit University, Bangkok, 10700, Thailand; email: rittipun_run@dusit.ac.th
    Rhyncholaeliocattleya Haw Yuan Beauty (Rlc. Haw Yuan Beauty) is a traditional orchid in tropical and subtropical Asian regions, which has attracted attention for its fragrant flowers with diverse colors. This study aimed to investigate the effects of different extraction methods on the phenolic profile, total flavonoid and cyanidin-3-O-glucoside contents, and anti-aging properties of Rlc. Haw Yuan Beauty crude extracts. The crude extracts were obtained using solvent, ultrasonic, and supercritical carbon dioxide fluid extraction methods. The major phenolic compounds including gallic, chlorogenic, and caffeic acids were present in all the extracts. The extract obtained with solvent extraction showed the highest total flavonoid content, whereas that obtained with supercritical fluid extraction yielded the highest cyanidin-3-O-glucoside content. Additionally, the different extraction methods had a significant influence on the antioxidant, anti-elastase, and anti-collagenase activities (p < 0.05) of Rlc. Haw Yuan Beauty extracts. The crude extracts derived from the ultrasonic and supercritical carbon dioxide fluid extraction methods were not cytotoxic to human skin fibroblasts at any of the tested concentrations (31Ð500 µg/ml). Thus, the crude extracts from Rlc. Haw Yuan Beauty could be a safe and active ingredient in the production of skincare products in the cosmetic industry for human use. © 2021 Science Society of Thailand under Royal Patronage. All rights reserved.
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    In vitro production of functional immune cells derived from human hematopoietic stem cells
    (Leibniz Research Centre for Working Environment and Human Factors, 2015) Witchuda Payuhakrit; Tasanee Panichakul; Natthawut Charoenphon; Panus Chalermsaenyakorn; Adithep Jaovisidha; Chokdee Wongborisuth; Rachanee Udomsangpetch
    Hematopoietic stem cells (HSC) from cord blood are potentially high sources for transplantation due to their low immunogenicity and the presence of the multipotent cells. These cells are capable of differentiating to produce various lineages of blood cells under specific conditions. We have enriched highly purified CD34+ cells from cord blood, determined in vitro growth of the cells in culture systems in the absence (condition A) or presence of GM-CSF and G-CSF (condition B), and determined the profile of immune cells during the period of cultivation by using flow cytometry. PhytohemagglutininA (PHA) was used as a mitogen to stimulate T lymphocytes derived from hematopoietic stem cells. GM-CSF and G-CSF prolonged the survival of the growing cells and also maintained expansion of cells in blastic stage. By day 12 of cultivation, when cell numbers peaked, various types of immune cells had appeared (CD14+ cells, CD40+HLA-DR+ cells, CD3+CD56+ cells, CD19+ cells, CD3+CD4+ cells, CD3+CD8+cells and CD3-CD56+). A significantly higher percentage of monocytes (p = 0.002) were observed under culture with GM-CSF, G-CSF when compared with culture without GM-CSF, G-CSF. In addition, T lymphocytes derived from HSC responded to 50 _g/ml of PHA. This is the first report showing the complete differentiation and proliferation of immune cells derived from CD34+ HSC under in vitro culture conditions. Lymphocytes, monocytes, dendritic cells and polymorph nuclear cells derived from HSC in vitro are unique, and thus may benefit various studies such as innate immunity and pathophysiology of immune disorders. � 2015, Leibniz Research Centre for Working Environment and Human Factors. All right reserved.
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    Liposomes Encapsulating Artocarpus lakoocha Roxb. and Glycyrrhiza glabra L. Extracts: Characterization and Shelf Life of Freeze-Dried Vesicles
    (Research and Development Institute Suan Dusit University, 2020) Tasanee Panichakul; Piyawan Youdee; Nattaporn Boohuad; Khwunjit Itsarasook; Prasit Suwannalert; T. Panichakul; Department of Cosmetic Science, Faculty of Science and Technology, Suan Dusit University, Bangkok, 10300, Thailand; email: tasanee_p@yahoo.com
    Liposome is the one way of encapsulation of extracts for reducing the extract degradation. This study was to prepare the liposome entrapped extracts of Artocarpus lakoocha Roxb. (L-Al), Glycyrrhiza glabra L. (L-Gg) alone and in combination of A. lakoocha and G. glabra extracts (L-AlGg). The liposomes were prepared by Mechanochemical method and freeze-drying. For stability of liposomes, storage at 4, 25 and 45¡C for 8 weeks was performed. The trapping efficiency of liposomes and tyrosinase inhibitory activities of extracts entrapped in liposome were investigated. Results showed liposome morphology was the spherical vesicles evaluated by TEM. Before freeze-drying, liposomes had particle sizes of 156.966 ± 0.808, 140.8 ± 0.818 and 158.633 ± 4.193 nm for L-Al, L-Gg and L-AlGg, respectively. The entrapment efficiency of L-Al, L-Gg and L-AlGg was found to be 95.83 ± 13.48, 97.99 ± 5.23 and 93.90 ± 16.28 %, respectively. The tyrosinase inhibitory activities of released extracts from L-Al, L-Gg and L-AlGg were 81.57 ± 1.22, 68.92 ± 1.23 and 81.40 ± 0.64 %, respectively. After freeze-drying, the particle sizes of L-Al and L-AlGg were no significant changes, while L-Gg particle size was bigger (p < 0.01). The liposome entrapment and tyrosinase inhibitory activity of released extracts were not significantly changed after freeze-drying. This indicates good stability and no extract leakage of liposomes. In storage at 4¡C for 8 weeks, the entrapment efficiency of L-Al, L-Gg, L-AlGg and tyrosinase inhibitory activity of released extracts were not significantly different, comparing with controls. When increasing temperature of storage effected on the significantly reduction of the entrapment of liposomes and the tyrosinase inhibitory activity of released extracts (p < 0.01). Therefore, the freeze-dried liposome and storage at low temperature is recommended for stabilizing liposome and extract quality. © 2020, Research and Development Institute Suan Dusit University. All rights reserved.
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    Liposomes Encapsulating Artocarpus lakoocha Roxb. and Glycyrrhiza glabra L. Extracts: Characterization and Shelf Life of Freeze-Dried Vesicles
    (Graphicsite, 2023-09-26) Tasanee Panichakul; Piyawan Youdee; Nattaporn Boohuad; Khwunjit Itsarasook; Prasit Suwannalert
    Liposome is the one way of encapsulation of extracts for reducing the extract degradation. This study was to prepare the liposome entrapped extracts of Artocarpus lakoocha Roxb. (L-Al), Glycyrrhiza glabra L. (L-Gg) alone and in combination of A. lakoocha and G. glabra extracts (L-AlGg). The liposomes were prepared by Mechanochemical method and freeze-drying. For stability of liposomes, storage at 4, 25 and 45°C for 8 weeks was performed. The trapping efficiency of liposomes and tyrosinase inhibitory activities of extracts entrapped in liposome were investigated. Results showed liposome morphology was the spherical vesicles evaluated by TEM. Before freeze-drying, liposomes had particle sizes of 156.966 ± 0.808, 140.8 ± 0.818 and 158.633 ± 4.193 nm for L-Al, L-Gg and L-AlGg, respectively. The entrapment efficiency of L-Al, L-Gg and L-AlGg was found to be 95.83 ± 13.48, 97.99 ± 5.23 and 93.90 ± 16.28 %, respectively. The tyrosinase inhibitory activities of released extracts from L-Al, L-Gg and L-AlGg were 81.57 ± 1.22, 68.92 ± 1.23 and 81.40 ± 0.64 %, respectively. After freeze-drying, the particle sizes of L-Al and L-AlGg were no significant changes, while L-Gg particle size was bigger (p < 0.01). The liposome entrapment and tyrosinase inhibitory activity of released extracts were not significantly changed after freezedrying. This indicates good stability and no extract leakage of liposomes. In storage at 4°C for 8 weeks, the entrapment efficiency of L-Al, L-Gg, L-AlGg and tyrosinase inhibitory activity of released extracts were not significantly different, comparing with controls. When increasing temperature of storage effected on the significantly reduction of the entrapment of liposomes and the tyrosinase inhibitory activity of released extracts (p < 0.01). Therefore, the freeze-dried liposome and storage at low temperature is recommended for stabilizing liposome and extract quality.
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    Phosphoproteomic analysis of apoptotic hematopoietic stem cells from hemoglobin E/_-thalassemia
    (2011) Saranyoo Ponnikorn; Tasanee Panichakul; Kitima Sresanga; Chokdee Wongborisuth; Sittiruk Roytrakul; Suradej Hongeng; Sumalee Tungpradabkul; S. Hongeng; Department of Pediatrics, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand; email: rashe@mahidol.ac.th
    Background: Hemoglobin E/_-thalassemia is particularly common in Southeast Asia and has variable symptoms ranging from mild to severe anemia. Previous investigations demonstrated the remarkable symptoms of _-thalassemia in terms of the acceleration of apoptotic cell death. Ineffective erythropoiesis has been studied in human hematopoietic stem cells, however the distinct apoptotic mechanism was unclear.Methods: The phosphoproteome of bone marrow HSCs/CD34+ cells from HbE/_-thalassemic patients was analyzed using IMAC phosphoprotein isolation followed by LC-MS/MS detection. Decyder MS software was used to quantitate differentially expressed proteins in 3 patients and 2 normal donors. The differentially expressed proteins from HSCs/CD34+ cells were compared with HbE/_-thalassemia and normal HSCs.Results: A significant change in abundance of 229 phosphoproteins was demonstrated. Importantly, the analysis of the candidate proteins revealed a high abundance of proteins that are commonly found in apoptotic cells including cytochrome C, caspase 6 and apoptosis inducing factors. Moreover, in the HSCs patients a significant increase was observed in a specific type of phosphoserine/threonine binding protein, which is known to act as an important signal mediator for the regulation of cell survival and apoptosis in HbE/_-thalassemia.Conclusions: Our study used a novel method to investigate proteins that influence a particular pathway in a given disease or physiological condition. Ultimately, phosphoproteome profiling in HbE/_-thalassemic stem cells is an effective method to further investigate the cell death mechanism of ineffective erythropoiesis in _-thalassemia. Our report provides a comprehensive phosphoproteome, an important resource for the study of ineffective erythropoiesis and developing therapies for HbE/_-thalassemia. © 2011 Ponnikorn et al; licensee BioMed Central Ltd.
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    Plasmodium vivax inhibits erythroid cell growth through altered phosphorylation of the cytoskeletal protein ezrin
    (BioMed Central Ltd., 2015) Tasanee Panichakul; Saranyoo Ponnikorn; Sittiruk Roytrakul; Atchara Paemanee; Suthathip Kittisenachai; Suradej Hongeng; Rachanee Udomsangpetch
    Background: The underlying causes of severe malarial anaemia are multifactorial. In previously reports, Plasmodium vivax was found to be able to directly inhibited erythroid cell proliferation and differentiation. The molecular mechanisms underlying the suppression of erythropoiesis by P. vivax are remarkably complex and remain unclear. In this study, a phosphoproteomic approach was performed to dissect the molecular mechanism of phosphoprotein regulation, which is involved in the inhibitory effect of parasites on erythroid cell development. Methods: This study describes the first comparative phosphoproteome analysis of growing erythroid cells (gECs), derived from human haematopoietic stem cells, exposed to lysates of infected erythrocytes (IE)/uninfected erythrocytes (UE) for 24, 48 and 72 h. This study utilized IMAC phosphoprotein isolation directly coupled with LC MS/MS analysis. Results: Lysed IE significantly inhibited gEC growth at 48 and 72 h and cell division resulting in the accumulation of cells in G0 phase. The relative levels of forty four phosphoproteins were determined from gECs exposed to IE/UE for 24-72 h and compared with the media control using the label-free quantitation technique. Interestingly, the levels of three phosphoproteins: ezrin, alpha actinin-1, and Rho kinase were significantly (p_<_0.05) altered. These proteins display interactions and are involved in the regulation of the cellular cytoskeleton. Particularly affected was ezrin (phosphorylated at Thr567), which is normally localized to gEC cell extension peripheral processes. Following exposure to IE, for 48-72 h, the ezrin signal intensity was weak or absent. This result suggests that phospho-ezrin is important for actin cytoskeleton regulation during erythroid cell growth and division. Conclusions: These findings suggest that parasite proteins are able to inhibit erythroid cell growth by down-regulation of ezrin phosphorylation, leading to ineffective erythropoiesis ultimately resulting in severe malarial anaemia. A better understanding of the mechanisms of ineffective erythropoiesis may be beneficial in the development of therapeutic strategies to prevent severe malarial anaemia. � 2015 Panichakul et al.; licensee BioMed Central.
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    Preparation and Adsorption Properties of a Biosorbent from Banana Peel for Use as Natural Vitamin Beads in Cosmetic Products
    (Research and Development Institute Suan Dusit University, 2022) Pornpassanan Dechprasitichok; Tasanee Panichakul; Khwunjit Itsarasook; Napaporn Boonthoem; Christoph Sontag; Sanchai Luachan; P. Dechprasitichok; Faculty of Science, Suan Dusit University, Bangkok, 10300, Thailand; email: paunpassanan_dec@dusit.ac.th
    The purpose of this research was to produce natural vitamin beads using a biosorbent from banana peel as an alternative to plastic vitamin beads for use in cosmetic products. The new biosorbents could be prepared by an extraction process in combination with a hydrothermal technique and physical processing. The biosorbent material has high fiber content, up to 45.25% by weight, particle sizes in the range of 10-160 _m, with a specific surface area of 21.5 m2/g and a point of zero charge at pH 6.83. It has a high cellulose crystallinity index (Icr) equal to 59.2%. It could be manufactured with a yield of 8.85%. The study on the adsorption equilibrium of this biosorbent material showed that the Langmuir isotherm fits better for the adsorption process (R2 = 0.9912) than the Freundlich isotherm (R2 = 0.9532) which presented a monolayer surface adsorption mechanism confirmed by XRD of vitamin C from released solution. The biosorbent from banana peel has an effective adsorption capacity for vitamin C (5% solution) of 545 mg/g and the release efficiency of vitamin C was 80% in water. In addition, an increase of adsorption capacity from 27 to 50 ¡C showed that the adsorption reaction between the biosorbent and vitamin C was endothermic. We have concluded that biosorbent from banana peel can be prepared by a hydrothermal method that is energy-efficient and environmentally friendly. This biosorbent material can be used as a natural alternative to polyethylene beads for vitamin C release in cosmetic products for antioxidant effect. The product from this research is a new category that combines natural materials with active ingredients to be used in cosmetic applications to ensure health safety and environmental protection. © 2022, Research and Development Institute Suan Dusit University. All rights reserved.
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    Preparation and Adsorption Properties of a Biosorbent from Banana Peel for Use as Natural Vitamin Beads in Cosmetic Products
    (Graphicsite, 2023-09-26) Pornpassanan Dechprasitichok; Tasanee Panichakul; Khwunjit Itsarasook; Napaporn Boonthoem; Christoph Sontag; Sanchai Luachan
    The purpose of this research was to produce natural vitamin beads using a biosorbent from banana peel as an alternative to plastic vitamin beads for use in cosmetic products. The new biosorbents could be prepared by an extraction process in combination with a hydrothermal technique and physical processing. The biosorbent material has high fiber content, up to 45.25% by weight, particle sizes in the range of 10-160 μm, with a specific surface area of 21.5 m2/g and a point of zero charge at pH 6.83. It has a high cellulose crystallinity index (Icr) equal to 59.2%. It could be manufactured with a yield of 8.85%. The study on the adsorption equilibrium of this biosorbent material showed that the Langmuir isotherm fits better for the adsorption process (R2 = 0.9912) than the Freundlich isotherm (R2= 0.9532) which presented a monolayer surface adsorption mechanism confirmed by XRD of vitamin C from released solution. The biosorbent from banana peel has an effective adsorption capacity for vitamin C (5% solution) of 545 mg/g and the release efficiency of vitamin C was 80% in water. In addition, an increase of adsorption capacity from 27 to 50 °C showed that the adsorption reaction between the biosorbent and vitamin C was endothermic. We have concluded that biosorbent from banana peel can be prepared by a hydrothermal method that is energy-efficient and environmentally friendly. This biosorbent material can be used as a natural alternative to polyethylene beads for vitamin C release in cosmetic products for antioxidant effect. The product from this research is a new category that combines natural materials with active ingredients to be used in cosmetic applications to ensure health safety and environmental protection.
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    Skin Anti-Aging Potential of Ipomoea pes-caprae Ethanolic Extracts on Promoting Cell Proliferation and Collagen Production in Human Fibroblasts (CCD-986sk Cells)
    (MDPI, 2022) Tasanee Panichakul; Saranyoo Ponnikorn; Wipa Tupchiangmai; Woraphot Haritakun; Kitima Srisanga; T. Panichakul; Department of Cosmetic Science, Faculty of Science and Technology, Suan Dusit University, Bangkok, 228-228/1-3 Sirindhorn Rd. Bangphlat, 10700, Thailand; email: tasanee_pan@dusit.ac.th
    Collagen loss in the skin dermis is a major cause of age-related changes to the skin. Natural phytochemical substances are desirable for the prevention of skin aging and the formation of wrinkles. Ipomoea pes-caprae (IPC) has been utilized for nutritional and therapeutic purposes, and its extract contains collagenase inhibitory activity while causing no cytotoxicity. The purpose of this study was to examine the impact of IPC extracts on cell proliferation and collagen production in human fibroblasts (CCD-986sk cells). IPC leaves were macerated in 70% and 95% ethanol and the chemical composition of the resulting extracts (IPC70 and IPC95) were determined using high performance liquid chromatography (HPLC). The bioactivity of IPC extracts was examined in CCD-986sk cells, including antioxidant capacity, inhibition of collagenase, effects on cell proliferation and collagen production, as well as wound healing using an in vitro scratch test. Changes in expression of collagen type I (COL1A1), tumor growth factor beta 1 (TGFB1), and beta-fibroblast growth factor (FGF2) genes were also evaluated. The antioxidant and collagenase inhibitory properties of IPC extracts were associated with 3,5-di-caffeoylquinic acid, chlorogenic acid, and ferulic acid. IPC extracts at noncytotoxic concentrations significantly increased cell proliferation, collagen production, and wound healing. These effects appear linked to the upregulation of COL1A1, TGFB1, and FGF2 genes. The bioactivity of the IPC70 extract was greater than that for IPC95. This is useful in cosmeceutical applications for human skin aging. Our findings indicate that IPC extracts have the potential for use in skin anti-aging cosmeceutical preparations. © 2022 by the authors.
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    Small rna interference inhibition of ubiquitin-specific protease 14 gene expression in intrahepatic cholangiocarcinoma primary cells
    (SEAMEO TROPMED Network, 2020) Ubol Chuensumran; Tasanee Panichakul; Thun Ingkakul; Poom Adisakwattana; U. Chuensumran; Department of Food Processing Technology, School of Culinary Arts, Suan Dusit University, Bangkok, 10300, Thailand; email: ubol_chu@dusit.ac.th
    Studies on genomic instabilities of intrahepatic cholangiocarcinoma (ICC) from Thai patients have shown 52% variations in a specific region of ubiquitin-specific protease 14 gene (USP14) located on 18p11.32. Inhibition of USP14 expression in three different primary cells cultured from resected ICC tissues by transfecting with double-strand RNA interference targeting exon 16 region of USP14 mRNA resulted in 6-50% reduction in mRNA levels, with cells with low USP14 expression being more affected. These findings lend support to a potential in developing strategies against USP14 in ICC. © 2020, SEAMEO TROPMED Network. All rights reserved.
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    Suppression of erythroid development in vitro by Plasmodium vivax
    (2012) Tasanee Panichakul; Witchuda Payuhakrit; Panyu Panburana; Chokdee Wongborisuth; Suradej Hongeng; Rachanee Udomsangpetch; T. Panichakul; Faculty of Science and Technology, Suan Dusit Rajabhat University, Bangplat, Bangkok, 10700, 204/3 Sirindhorn Rd., Thailand; email: tasanee_p@yahoo.com
    Background: Severe anaemia due to dyserythropoiesis has been documented in patients infected with Plasmodium vivax, however the mechanism responsible for anaemia in vivax malaria is poorly understood. In order to better understand the role of P. vivax infection in anaemia the inhibition of erythropoiesis using haematopoietic stem cells was investigated. Methods: Haematopoietic stem cells/CD34 + cells, isolated from normal human cord blood were used to generate growing erythroid cells. Exposure of CD34 + cells and growing erythroid cells to P. vivax parasites either from intact or lysed infected erythrocytes (IE) was examined for the effect on inhibition of cell development compared with untreated controls. Results: Both lysed and intact infected erythrocytes significantly inhibited erythroid growth. The reduction of erythroid growth did not differ significantly between exposure to intact and lysed IE and the mean growth relative to unexposed controls was 59.4 ± 5.2 for lysed IE and 57 ± 8.5% for intact IE. Interestingly, CD34 + cells/erythroid progenitor cells were susceptible to the inhibitory effect of P. vivax on cell expansion. Exposure to P. vivax also inhibited erythroid development, as determined by the reduced expression of glycophorin A (28.1%) and CD 71 (43.9%). Moreover, vivax parasites perturbed the division of erythroid cells, as measured by the Cytokinesis Block Proliferation Index, which was reduced to 1.35 ± 0.05 (P-value²0.01) from a value of 2.08 ± 0.07 in controls. Neither TNF-a nor IFN-g was detected in the culture medium of erythroid cells treated with P. vivax, indicating that impaired erythropoiesis was independent of these cytokines. Conclusions: This study shows for the first time that P. vivax parasites inhibit erythroid development leading to ineffective erythropoiesis and highlights the potential of P. vivax to cause severe anaemia. © 2012 Panichakul et al.; licensee BioMed Central Ltd.