Antioxidant Activities and Cytotoxicity Effect on Normal Human Dermal Fibroblasts of Roselle (Hibiscus sabdariffa L.) Calyces Extracts and W/O/W Emulsion Loaded Extract for Cosmetic Applications

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dc.contributor.authorKhwunjit Itsarasook
dc.contributor.authorPiyanuch Prompamorn
dc.contributor.authorJittarawadee Tanghiranrat
dc.contributor.authorKanlayaporn Chantree
dc.contributor.authorItsaret Itsarasuk
dc.contributor.correspondenceK. Itsarasook; Faculty of Science and Technology, Suan Dusit University, Bangkok, 10700, Thailand; email: khwunjit_its@dusit.ac.th
dc.date.accessioned2025-03-10T07:34:45Z
dc.date.available2025-03-10T07:34:45Z
dc.date.issued2023
dc.description.abstractThe objective of this study was to evaluate the antioxidant activities and cytotoxicity effect on normal human dermal fibroblasts of roselle (Hibiscus sabdariffa L.) calyx extracts and to load with the roselle extract in the inner phase of water in oil in water multiple emulsion for application in facial cosmetic. The extracts of dried roselle calyces were prepared by a maceration technique using ethanol/water at concentrations of 30%, 70%, 95% and 0% (100% DI water). Obtained results showed that the 30% ethanolic extract had a higher amount of total phenolic and anthocyanin content than other extracts. According to the DPPH and ABTS assays, the 30% ethanolic extract exhibited dose-dependent antioxidant activity higher than other extracts with an IC50 value of 0.432±0.001 mg/mL and 0.0855±0.01 mg/mL, respectively. The formulation of water in oil in water multiple emulsion containing the roselle calyx extract was prepared by a beaker method with two-step emulsification. The prepared multiple emulsion showed a light pink color with homogeneously formula. The physiochemical stability of the prepared formula was evaluated at accelerated conditions; room temperature (30±5¡C), low temperature (4±1¡C) and high temperature (40±1¡C) for 30 days and 6 cycles of freeze (-25¡C, 24 h)-thaw (4¡C, 24 h). Results found that the tested formula showed a good physicochemical stability compared to the initial condition. All finding suggests possible application for the 30% ethanolic extract of roselle calyces as a natural active ingredient in skin cosmetic products. © 2023, Research and Development Institute Suan Dusit University. All rights reserved.
dc.identifier.citationJournal of Food Health and Bioenvironmental Science
dc.identifier.issn26300311
dc.identifier.scopus2-s2.0-85207312175
dc.identifier.urihttps://repository.dusit.ac.th//handle/123456789/4559
dc.languageEnglish
dc.publisherResearch and Development Institute Suan Dusit University
dc.rights.holderScopus
dc.subjectAntioxidation activity
dc.subjectCytotoxicity study
dc.subjectRoselle calyx extracts
dc.subjectWater in oil in water emulsion
dc.titleAntioxidant Activities and Cytotoxicity Effect on Normal Human Dermal Fibroblasts of Roselle (Hibiscus sabdariffa L.) Calyces Extracts and W/O/W Emulsion Loaded Extract for Cosmetic Applications
dc.typeArticle
mods.location.urlhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85207312175&partnerID=40&md5=d8fd55c763f3f48fb558776b24ee898d
oaire.citation.endPage48
oaire.citation.issue2
oaire.citation.startPage38
oaire.citation.volume16
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