Ubiquitin-specific protease 14 expression associated with intrahepatic cholangiocarcinoma cell differentiation

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dc.contributor.authorUbol Chuensumran
dc.contributor.authorPensri Saelee
dc.contributor.authorPhaibul Punyarit
dc.contributor.authorSopit Wongkham
dc.contributor.authorChawalit Pairojkul
dc.contributor.authorSiri Chauin
dc.contributor.authorSongsak Petmitr
dc.contributor.correspondenceU. Chuensumran; Department of Chemistry, Faculty of Science and Technology, Rajabhat University Suan Dusit, Thailand; email: ubol_c@yahoo.com
dc.date.accessioned2025-03-10T07:37:40Z
dc.date.available2025-03-10T07:37:40Z
dc.date.issued2011
dc.description.abstractThe purpose of this study was to identify the gene alterations amplified from AO16 primer and examine whether the expression patterns of USP14 in clinical specimens from patients with intrahepatic cholangiocarcinoma (ICC) is associated with cancer cells. DNA from tumor and corresponding normal tissues of 52 patients was amplified with 33 arbitrary primers. The DNA fragment that altered most frequently in ICC was cloned, sequenced, and identified by comparison with known nucleotide sequences in the genome database. The DNA copy numbers of the allelic alterations in cholangiocarcinoma were determined by quantitative real-time PCR and interpreted as allelic loss or DNA amplification by comparison with the reference gene. Associations between allelic imbalance and clinicopathological parameters of ICC patients were evaluated by _2-tests. The Kaplan-Meier method was used to analyze survival rates. Immunohistochemically, USP14 showed weak cytoplasmic staining in normal bile duct epithelial cells. It was strongly detected in 21 cancer patients (43.8%). There were correlations between USP14 expression level and the clinicopathological features of ICC, histological grade (P < 0.05). However, there were no significant differences in age, gender, tumor size, metastasis, lymph node metastasis, and staging. USP14 expression was related to cholangiocarcinoma cell differentiation. Due to their emerging role in control of multiple signaling pathways and oncoproteins, USP14 inhibitors may be useful for anticancer agents.
dc.identifier.citationAsian Pacific Journal of Cancer Prevention
dc.identifier.issn15137368
dc.identifier.scopus2-s2.0-79958798500
dc.identifier.urihttps://repository.dusit.ac.th//handle/123456789/5009
dc.languageEnglish
dc.publisherAsian Pacific Organization for Cancer Prevention
dc.rights.holderScopus
dc.subjectCell differentiation
dc.subjectIntrahepatic cholangiocarcinoma
dc.subjectUbiquitin-specific protease 14
dc.titleUbiquitin-specific protease 14 expression associated with intrahepatic cholangiocarcinoma cell differentiation
dc.typeArticle
mods.location.urlhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-79958798500&partnerID=40&md5=b3523fbaa645cfde79f4fe3840c32f00
oaire.citation.endPage779
oaire.citation.issue3
oaire.citation.startPage775
oaire.citation.volume12
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