Production of poly (l-lactide)-degrading enzyme by Actinomadura keratinilytica strain T16-1 under solid state fermentation using agricultural wastes as substrate

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dc.contributor.authorSukhumaporn Krajangsang
dc.contributor.authorNuttanisa Dechsresawut
dc.contributor.authorThanasak Lomthong
dc.contributor.authorSrisuda Samaimai
dc.contributor.correspondenceS. Samaimai; Faculty of Science and Technology, Suan Dusit University, Bangkok, 10700, Thailand; email: srisuda_sam@dusit.ac.th
dc.date.accessioned2025-03-10T07:35:29Z
dc.date.available2025-03-10T07:35:29Z
dc.date.issued2021
dc.description.abstractPoly (l-lactide) (PLLA) is an aliphatic polyester that can be obtained from renewable resources and degraded by various microorganisms. In previous reports, Actinomadura keratinilytica strain T16-1 demonstrated high ability to degrade PLLA under various conditions. PLLA-degrading enzyme production under solid state fermentation has been sparsely studied. PLLA-degrading enzyme production by A. keratinilytica strain T16-1 was investigated using agricultural wastes as substrate under solid state fermentation (SSF). Three agricultural wastes as soybean meal, cassava chips and duckweed were tested as substrates for PLLA-degrading enzyme production by statistical methods using mixture design. Results revealed that using duckweed as the substrate gave the highest enzyme production (138.66 ± 13.57 U/g dry substrate). Maximum enzyme activity of 391.24 ± 15.57 U/g dry substrate was obtained under 10Êg duckweed, 10% inoculum size, 7Êdays of cultivation time, pH 7.0, 2.8% PLLA powder, and 60% moisture content at 45Ê¡C. It can be concluded that duckweed is an inexpensive substrate, which reduces the costs of PLLA-degrading enzyme production, as an alternative to effective water weed management. © 2021, King Abdulaziz City for Science and Technology.
dc.identifier.citation3 Biotech
dc.identifier.doi10.1007/s13205-021-03060-8
dc.identifier.issn2190572X
dc.identifier.scopus2-s2.0-85119969652
dc.identifier.urihttps://repository.dusit.ac.th//handle/123456789/4717
dc.languageEnglish
dc.publisherSpringer Science and Business Media Deutschland GmbH
dc.rightsAll Open Access; Green Open Access
dc.rights.holderScopus
dc.subjectActinomadura keratinilytica
dc.subjectDuckweed
dc.subjectPoly (l-lactide) (PLLA)-degrading enzyme
dc.subjectSolid state fermentation
dc.titleProduction of poly (l-lactide)-degrading enzyme by Actinomadura keratinilytica strain T16-1 under solid state fermentation using agricultural wastes as substrate
dc.typeArticle
mods.location.urlhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85119969652&doi=10.1007%2fs13205-021-03060-8&partnerID=40&md5=05d5d695022c60504b4d9dd7d9d93ff7
oaire.citation.issue12
oaire.citation.volume11
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