RASSF1A promoter hypermethylation as a prognostic marker for hepatocellular carcinoma

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dc.contributor.authorPensri Saelee
dc.contributor.authorSopit Wongkham
dc.contributor.authorSunanta Chariyalertsak
dc.contributor.authorSongsak Petmitr
dc.contributor.authorUbol Chuensumran
dc.contributor.correspondenceP. Saelee; Research Division, National Cancer Institute, Bangkok, Thailand; email: saelee@health.moph.go.th
dc.date.accessioned2025-03-10T07:37:41Z
dc.date.available2025-03-10T07:37:41Z
dc.date.issued2010
dc.description.abstractThis study was performed to determine whether epigenetic aberrant methylation of RASSF1A might be associated with hepatocarcinogenesis. Methylation specific-PCR was performed to identify RASSF1A promoter hypermethylation in 29 tumors and corresponding normal liver tissues. In addition, RASSF1A mRNA levels were analyzed by quantitative real-time reverse transcription-PCR. Aberrant methylation of RASSF1A was detected in 25 of 29 cases (86%), with loss of RASSF1A expression evident in 8 of 22 cases (36%). No correlation between loss of RASSF1A mRNA and promoter hypermethylation of the RASSF1A gene was observed. There was a significant correlation between the methylation status of RASSF1A and hepatocellular carcinoma (HCC) patients who did not undergo chemotherapy (P = 0.03). Multivariate analysis, adjusted for tumor size, treatment, RASSF1A hypermethylation, and RASSF1A under-expression, showed RASSF1A hypermethylation to be assocaited with a better prognosis for HCC patients (HR= 0.089, 95%CI = 0.013-0.578; P = 0.012). Our findings showed that RASSF1A promoter hypermethylation occurs frequently, and may serve as a good prognostic factor.
dc.identifier.citationAsian Pacific Journal of Cancer Prevention
dc.identifier.issn15137368
dc.identifier.scopus2-s2.0-79956136713
dc.identifier.urihttps://repository.dusit.ac.th//handle/123456789/5037
dc.languageEnglish
dc.publisherAsian Pacific Organization for Cancer Prevention
dc.rights.holderScopus
dc.subjectDNA methylation
dc.subjectHepatocellular carcinoma
dc.subjectMethylation-specific PCR
dc.subjectRASSF1A
dc.subjectThailand
dc.titleRASSF1A promoter hypermethylation as a prognostic marker for hepatocellular carcinoma
dc.typeArticle
mods.location.urlhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-79956136713&partnerID=40&md5=f76b32c66887cebf872f24297d4fa3f3
oaire.citation.endPage1681
oaire.citation.issue6
oaire.citation.startPage1677
oaire.citation.volume11
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