Effects of Nutrient Supplement and Chitosan on Microbial Population Change in Up-Flow-Anaerobic-Sludge-Blanket Reactor during Biogas Production

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dc.contributor.authorRungroj Piyaphanuwat
dc.contributor.authorSrisuda Samaimai
dc.contributor.authorVassanasak Limkhuansuwan
dc.contributor.correspondenceV. Limkhuansuwan; Faculty of Science and Technology, Suan Dusit University, Bangkok, 10700, Thailand; email: vassanasak_lim@dusit.ac.th
dc.date.accessioned2025-03-10T07:35:29Z
dc.date.available2025-03-10T07:35:29Z
dc.date.issued2021
dc.description.abstractThe objective of this research was to study the effects of nutrient supplement and chitosan on microbial change in an up-flow-anaerobic-sludge-blanket (UASB) reactor during biogas production. Three UASB reactors were operational in this study. All reactors were operated by feeding dilute stillage with chemical oxygen demand (COD) concentration at 10,000 mg/L and 9 days of hydraulic retention time (HRT) under anaerobic conditions. Reactor 2 and reactor 3 were supplemented with nutrient supplement and chitosan, respectively. The results of the environment and pH values of all UASB reactors showed similar conditions with total volatile acid/ alkalinity (TVA/Alk) values of 0.27-0.31. The COD removal efficiency of reactor 1 (stillage), 2 (stillage and nutrient supplement) and 3 (stillage and chitosan) showed about 79%, 84% and 87%, respectively. In addition, it was found that the UASB reactors supplemented with nutrient supplement or chitosan produced higher levels of biogas than those without additives. The 16S rRNA technique by PCR reaction showed that the dominant archaea in the final fermentation of all UASB reactors and in inoculum sample were hydrogenotrophic (genus Methanobacterium) and acetotrophic methanogens (genus Methanosaeta). The methanogens population in the reactor supplemented with chitosan (18.11%) produced more biogas than the ones in the reactor supplemented with nutrient supplement (14.44%) and in the control reactor (15.95%). © 2021, Research and Development Institute Suan Dusit University. All rights reserved.
dc.identifier.citationJournal of Food Health and Bioenvironmental Science
dc.identifier.issn26300311
dc.identifier.scopus2-s2.0-85207280843
dc.identifier.urihttps://repository.dusit.ac.th//handle/123456789/4726
dc.languageEnglish
dc.publisherResearch and Development Institute Suan Dusit University
dc.rights.holderScopus
dc.subject16S rRNA
dc.subjectChitosan
dc.subjectMicrobial population
dc.subjectNutrient supplement
dc.subjectUp-flow-anaerobic-sludge-blanket reactor
dc.titleEffects of Nutrient Supplement and Chitosan on Microbial Population Change in Up-Flow-Anaerobic-Sludge-Blanket Reactor during Biogas Production
dc.typeArticle
mods.location.urlhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85207280843&partnerID=40&md5=dd86a579a2155d77170a622fd26554c0
oaire.citation.endPage11
oaire.citation.issue2
oaire.citation.startPage1
oaire.citation.volume14
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