Lipid Production of Marine Green Microalgae Chlorella protothecoides BUUC1601 by Using Spent Coffee Grounds Hydrolysate

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dc.contributor.authorRachanimuk Hiransuchalert
dc.contributor.authorNisa Siranonthana
dc.contributor.authorNuttapon Chedtaisong
dc.contributor.authorPakawan Setthamongkol
dc.contributor.authorYutthaya Yuyen
dc.contributor.authorJanjarus Watanachote
dc.contributor.authorPaveena Tapaneeyaworawong
dc.contributor.authorNongnuch Rungsawang
dc.contributor.authorMaliwan Kutako
dc.contributor.correspondenceM. Kutako; Faculty of Marine Technology, Burapha University, Chanthaburi, Chanthaburi Campus, 22170, Thailand; email: maliwan@buu.ac.th
dc.date.accessioned2025-03-10T07:34:45Z
dc.date.available2025-03-10T07:34:45Z
dc.date.issued2023
dc.description.abstractSpent coffee grounds are an organic waste that can be used as a source of microbial organic carbon. In this research, coffee grounds were hydrolyzed into a solution called spent coffee grounds hydrolysate (SCGH) using concentrated sulfuric acid. Then, the marine green microalgae, C. protothecoides BUUC1601, was cultured using SCGH. Growth performance and lipid accumulation of the microalgae were evaluated. The microalgae were cultured using a standard F/2 medium without and with SCGH added in the range of 2.5-15% of culture media (v/v). It was found that the microalgae had similar growth performance and biomass yield, i.e., the specific growth rate was in the range of 0.87-1.12 day-1 and the biomass yield was in the range of 0.05-0.08 g DW/L/day. Microalgae cultivation using F/2 with SCGH had an effect on lipid accumulation. It was found that using SCGH at 15% of the total volume resulted in an increase in the amount of lipid accumulation up to 66.03% of the dry weight. Of these, it was 2.89 times higher than the lipid content of microalgae cultured with no SCGH added. The content of monounsaturated fatty acids ranged from 46.15% to 46.53% and polyunsaturated fatty acids ranged from 32.40% to 34.62% of the total fatty acid content. Oleic acid (C18:1n9), an omega-9 fatty acid, was found to be the most abundant, accounting for over 30% of the total fatty acid content. In contrast, the omega-6 fatty acids linoleic acid (C18:2n6) and gamma-linolenic acid (C18:3n6) were found to be lower, accounting for 25.99%-27.20% and 6.03%-7.01% of the total fatty acid content, respectively. The omega-3 fatty acid such as alpha-linolenic acid (C18:3n3) was found at 4.20% in microalgae cultured using standard F/2 medium without SCGH, which was higher than in microalgae cultured with the addition of SCGH. Therefore, the addition of SCGH at a concentration of 2.5%-15% (v/v) can be used to cultivate C. protothecoides BUUC1601 for the lipid production with high unsaturated fatty acid content, which has the potential to be used in both aquaculture and functional food supplementation. © 2023, Research and Development Institute Suan Dusit University. All rights reserved.
dc.identifier.citationJournal of Food Health and Bioenvironmental Science
dc.identifier.issn26300311
dc.identifier.scopus2-s2.0-85207255329
dc.identifier.urihttps://repository.dusit.ac.th//handle/123456789/4566
dc.languageEnglish
dc.publisherResearch and Development Institute Suan Dusit University
dc.rights.holderScopus
dc.subjectChlorella protothecoides
dc.subjectFatty acid
dc.subjectLipid production
dc.subjectMarine green microalgae
dc.subjectSpent coffee grounds hydrolysate
dc.titleLipid Production of Marine Green Microalgae Chlorella protothecoides BUUC1601 by Using Spent Coffee Grounds Hydrolysate
dc.typeArticle
mods.location.urlhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85207255329&partnerID=40&md5=eddb8b0dc91f8737e75434860a87e9e1
oaire.citation.endPage45
oaire.citation.issue1
oaire.citation.startPage37
oaire.citation.volume16
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