Histological type of intrahepatic cholangiocarcinoma differentiated by genetic alteration from AP-PCR fingerprint

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dc.contributor.authorUbol Chuensumran
dc.contributor.authorPensri Saelee
dc.contributor.authorSopit Wongkham
dc.contributor.authorChawalit Pairojkul
dc.contributor.authorSiri Chauin
dc.contributor.authorSongsak Petmitr
dc.contributor.correspondenceU. Chuensumran; Department of Chemistry, Faculty of Science and Technology, Rajabhat University Suan Dusit, Bangkok, Thailand; email: ubolc@yahoo.com
dc.date.accessioned2025-03-10T07:37:40Z
dc.date.available2025-03-10T07:37:40Z
dc.date.issued2011
dc.description.abstractCholangiocarcinoma (CCA), the malignant neoplasm of the biliary epithelium, is usually fatal due to difficulty in early diagnosis and lack of availability of effective therapy. The genetic mechanisms involved in the development of CCA are not well understood and only a few cytogenetic studies have been published. In this study, genomic instability in 30 Thai cases of intrahepatic cholangiocarcinoma (ICC) was assessed using an arbitrarily primed- polymerase chain reaction (AP-PCR) method. Genetic alterations were analyzed as banding pattern changes between tumors and corresponding normal DNA. The abnormal band present at the highest frequency (23/30 cases, 77%) appeared with the AO16 primer. Statistical analysis also showed that DNA alteration from this primer was significantly associated with the moderately to poorly differentiated histological type (P = 0.038). Kaplan-Meier survival curves showed borderline significance for this DNA aberration (P = 0.06 by the log-rank test). This DNA fragment may thus be of use to predict degree of malignancy of the disease.
dc.identifier.citationAsian Pacific Journal of Cancer Prevention
dc.identifier.issn15137368
dc.identifier.scopus2-s2.0-84858031201
dc.identifier.urihttps://repository.dusit.ac.th//handle/123456789/5010
dc.languageEnglish
dc.publisherAsian Pacific Organization for Cancer Prevention
dc.rights.holderScopus
dc.subjectAP-PCR
dc.subjectCCA
dc.subjectGenetic alteration
dc.subjectICC
dc.subjectIntrahepatic cholangiocarcinoma
dc.subjectThailand
dc.titleHistological type of intrahepatic cholangiocarcinoma differentiated by genetic alteration from AP-PCR fingerprint
dc.typeArticle
mods.location.urlhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84858031201&partnerID=40&md5=d3d0d7d34a36b5d9caa0251215dbdbe1
oaire.citation.endPage1380
oaire.citation.issue6
oaire.citation.startPage1377
oaire.citation.volume12
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