Degradation of poly(Butylene succinate) and poly(butylene succinate)/poly(lactide) blends using serine protease produced from laceyella sacchari LP175

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dc.contributor.authorSrisuda Samaimai
dc.contributor.authorSukhumaporn Krajangsang
dc.contributor.authorVichien Kitpreechavanich
dc.contributor.authorJednipit Borthong
dc.contributor.authorThanasak Lomthong
dc.contributor.correspondenceT. Lomthong; Division of Biology, Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi, Pathum Thani, 12110, Thailand; email: thanasak_l@rmutt.ac.th
dc.date.accessioned2025-03-10T07:35:28Z
dc.date.available2025-03-10T07:35:28Z
dc.date.issued2021
dc.description.abstractThe thermophilic filamentous bacterium Laceyella sacchari LP175 was cultivated in a 10.0 L airlift fermenter to produce serine protease at 50 ¡C. Maximal serine protease activity at 1,123.32 ± 15.8 U/mL was obtained for cultivation at 0.6 vvm aeration rate for 36 h. The crude enzyme was applied for degradation of poly (butylene succinate) (PBS), and poly (butylene succinate)/poly(lactide) blend (PBS/PLA) powders at 50 ¡C for 48 h with different substrates and enzyme concentrations. Results showed that serine protease produced from L. sacchari LP175 degraded PBS and PBS/PLA at 46.5 ± 2.05 and 49.8 ± 1.45 %, respectively, at an initial substrate concentration of 100 g/L with 1,200 U/mL of serine protease activity. Percentage degradation of PBS and PBS/PLA was improved to 51.4 ± 1.06 and 56.9 ± 1.42 %, respectively, when upscaled in a 2.0 L stirrer fermenter with 200 rpm agitation rate. Degradation products evaluated by a scanning electron microscope (SEM) and Fourier transform infrared spectroscopy (FTIR) confirmed that serine protease produced from L. sacchari LP175 degraded both PBS and PBS/PLA polymers. Results showed that microbial enzyme technology could be used to degrade PBS and PBS/PLA blend polymers and reduce the accumulation of waste. © 2021, Walailak University. All rights reserved.
dc.identifier.citationTrends in Sciences
dc.identifier.doi10.48048/tis.2021.37
dc.identifier.issn27740226
dc.identifier.scopus2-s2.0-85121641152
dc.identifier.urihttps://repository.dusit.ac.th//handle/123456789/4679
dc.languageEnglish
dc.publisherWalailak University
dc.rightsAll Open Access; Hybrid Gold Open Access
dc.rights.holderScopus
dc.subjectBiodegradation
dc.subjectLaceyella sacchari LP175
dc.subjectPoly(butylene succinate) (PBS)
dc.subjectPolybutylene succinate/poly(lactide) (PBS/PLA) blend
dc.subjectSerine protease production
dc.titleDegradation of poly(Butylene succinate) and poly(butylene succinate)/poly(lactide) blends using serine protease produced from laceyella sacchari LP175
dc.typeArticle
mods.location.urlhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85121641152&doi=10.48048%2ftis.2021.37&partnerID=40&md5=503c775c4e8ab98ad3417e5c11a856b7
oaire.citation.issue20
oaire.citation.volume18
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