Antioxidant Activities and Cytotoxicity Effect on Normal Human Dermal Fibroblasts of Roselle (Hibiscus sabdariffa L.) Calyces Extracts and W/O/W Emulsion Loaded Extract for Cosmetic Applications

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dc.contributor.authorKhwunjit Itsarasook
dc.contributor.authorPiyanuch Prompamorn
dc.contributor.authorJittarawadee Tanghiranrat
dc.contributor.authorKanlayaporn Chantree
dc.contributor.authorItsaret Itsarasuk
dc.date.accessioned2025-04-21T07:59:25Z
dc.date.available2025-04-21T07:59:25Z
dc.date.issued2023-11-11
dc.description.abstractThe objective of this study was to evaluate the antioxidant activities and cytotoxicity effect on normal human dermal fibroblasts of roselle (Hibiscus sabdariffa L.) calyx extracts and to load with the roselle extract in the inner phase of water in oil in water multiple emulsion for application in facial cosmetic. The extracts of dried roselle calyces were prepared by a maceration technique using ethanol/water at concentrations of 30%, 70%, 95% and 0% (100% DI water). Obtained results showed that the 30% ethanolic extract had a higher amount of total phenolic and anthocyanin content than other extracts. According to the DPPH and ABTS assays, the 30% ethanolic extract exhibited dose-dependent antioxidant activity higher than other extracts with an IC50 value of 0.432±0.001 mg/mL and 0.0855±0.01 mg/mL, respectively. The formulation of water in oil in water multiple emulsion containing the roselle calyx extract was prepared by a beaker method with twostep emulsification. The prepared multiple emulsion showed a light pink color with homogeneously formula. The physiochemical stability of the prepared formula was evaluated at accelerated conditions; room temperature (30±5ºC), low temperature (4±1ºC) and high temperature (40±1ºC) for 30 days and 6 cycles of freeze (-25ºC, 24 h)-thaw (4ºC, 24 h). Results found that the tested formula showed a good physicochemical stability compared to the initial condition. All finding suggests possible application for the 30% ethanolic extract of roselle calyces as a natural active ingredient in skin cosmetic products.
dc.description.sponsorshipSuan Dusit University
dc.identifier.issn2630-0311
dc.identifier.urihttps://repository.dusit.ac.th//handle/123456789/6301
dc.language.isoen
dc.publisherGraphicsite
dc.relation.ispartofseriesVol. 16 No. 2 (2023): May - August
dc.subjectWater in oil in water emulsion
dc.subjectRoselle calyx extracts
dc.subjectAntioxidation activity
dc.subjectCytotoxicity study
dc.titleAntioxidant Activities and Cytotoxicity Effect on Normal Human Dermal Fibroblasts of Roselle (Hibiscus sabdariffa L.) Calyces Extracts and W/O/W Emulsion Loaded Extract for Cosmetic Applications
dc.typeArticle
mods.location.urlhttps://li01.tci-thaijo.org/index.php/sdust/article/view/260666/177180
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