Antibacterial, antioxidant, cytotoxic effects and GC-MS analysis of mangrove-derived Streptomyces achromogenes TCH4 extract

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dc.contributor.authorJanpen Tangjitjaroenkun
dc.contributor.authorWanchai Pluempanupat
dc.contributor.authorRungnapa Tangchitcharoenkhul
dc.contributor.authorWaraporn Yahayo
dc.contributor.authorRoongtawan Supabphol
dc.contributor.correspondenceJ. Tangjitjaroenkun; Department of Resources and Environment, Faculty of Science at Si Racha, Kasetsart, University, Chonburi, Thailand; email: janpen.ta@ku.th; R. Supabphol; Department of Physiology, Faculty of Medicine, Srinakharinwirot University, Bangkok, Thailand; email: roongtawans@gmail.com
dc.date.accessioned2025-03-10T07:35:29Z
dc.date.available2025-03-10T07:35:29Z
dc.date.issued2021
dc.description.abstractThe in vitro biological activities of the ethyl acetate extract of the culture filtrate from Streptomyces achromogenes TCH4 (TCH4 extract) were evaluated. The ethyl acetate extract of TCH4 produced a bacteriostatic effect against Enterobacter cloacae, Staphylococcus aureus, Staphylococcus saprophyticus, Bacillus subtilis, methicillin-resistant S. aureus (MRSA) and Klebsiella pneumoniae. The extract had bactericidal activity against S. aureus, S. saprophyticus, S. aureus (MRSA) and K. pneumoniae with minimum bactericidal concentration (MBC) values in the range of 500-1000 µg/mL. The total phenolic and flavonoid contents in TCH4 extract were 107.20±2.57 mg gallic acid equivalent (GAE)/g and 44.91±0.84 mg quercetin equivalent (QE)/g of dry extract. Antioxidant activity was determined by DPPH radical (IC50 299.64±6.83 µg/mL) and ABTS radical scavenging (IC50 65.53±0.95 _g/mL), and the ferric-reducing antioxidant power (FRAP) (822.76±9.12 mM FeSO4.7H2O/g dry extract) assays. TCH4 extract exhibited cytotoxic activity in the DU-145 cell line (IC50 9.36±0.37 _g/mL). Analysis of extract constituents by GC-MS revealed pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl) (36.85%), benzeneacetamide (23.76%), and deferoxamine (12.85%) as the major compounds, which have been reported to possess pharmaceutical properties. S. achromogenes TCH4 could be a potential source of bioactive metabolites with antibacterial, antioxidant and anticancer activities for pharmaceutical applications. © 2021 by the Serbian Biological Society
dc.identifier.citationArchives of Biological Sciences
dc.identifier.doi10.2298/ABS210320017T
dc.identifier.issn3544664
dc.identifier.scopus2-s2.0-85111399848
dc.identifier.urihttps://repository.dusit.ac.th//handle/123456789/4740
dc.languageEnglish
dc.publisherInstitut za Bioloska Istrazivanja
dc.rightsAll Open Access; Gold Open Access
dc.rights.holderScopus
dc.subjectantibacterial
dc.subjectantioxidant
dc.subjectcytotoxic
dc.subjectmangrove
dc.subjectStreptomyces achromogenes
dc.titleAntibacterial, antioxidant, cytotoxic effects and GC-MS analysis of mangrove-derived Streptomyces achromogenes TCH4 extract
dc.typeArticle
mods.location.urlhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85111399848&doi=10.2298%2fABS210320017T&partnerID=40&md5=cb521de9d255ad6375876f2489e00d94
oaire.citation.endPage235
oaire.citation.issue2
oaire.citation.startPage223
oaire.citation.volume73
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