Isolation and characterization of lead-tolerant Ochrobactrum intermedium and its role in enhancing lead accumulation by Eucalyptus camaldulensis

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dc.contributor.authorPiyaporn Waranusantigul
dc.contributor.authorHung Lee
dc.contributor.authorMaleeya Kruatrachue
dc.contributor.authorPrayad Pokethitiyook
dc.contributor.authorChoowong Auesukaree
dc.contributor.correspondenceC. Auesukaree; Department of Biology, Faculty of Science, Mahidol University, Bangkok 10400, Rama VI Road, Thailand; email: sccar@mahidol.ac.th
dc.date.accessioned2025-03-10T07:37:41Z
dc.date.available2025-03-10T07:37:41Z
dc.date.issued2011
dc.description.abstractIn this study, the potential of rhizospheric bacteria in promoting the growth and Pb accumulation by the woody plant Eucalyptus camaldulensis under hydroponic conditions was investigated for the first time. Three Pb-tolerant bacteria were isolated from the rhizosphere of E. camaldulensis grown in Pb-contaminated soils in the Bo Ngam Pb mine, Thailand. Based on analysis of partial 16S rRNA gene sequence, the three isolates were identified as Microbacterium paraoxydans BN-2, Ochrobactrum intermedium BN-3, and Bacillus fusiformis BN-4. Among these strains, O. intermedium BN-3 showed the highest tolerance to not only Pb but also Cd and Zn. After growth in the presence of Pb, the membranes of O. intermedium BN-3 cells exhibited an increase in unsaturated fatty acid levels but a decrease in fluidity. In hydroponic studies, inoculation of O. intermedium BN-3 significantly increased the biomass and Pb accumulation by E. camaldulensis compared to the uninoculated control. The results suggested the role of the natural rhizospheric bacteria localized to the root surface of E. camaldulensis in promoting Pb accumulation and plant growth. Our results indicate that O. intermedium BN-3 and other indigenous rhizospheric bacteria have the potential to improve the efficiency of phytoremediation of Pb-contaminated sites. © 2011 Elsevier Ltd.
dc.identifier.citationChemosphere
dc.identifier.doi10.1016/j.chemosphere.2011.06.086
dc.identifier.issn456535
dc.identifier.scopus2-s2.0-80055079145
dc.identifier.urihttps://repository.dusit.ac.th//handle/123456789/5032
dc.languageEnglish
dc.publisherElsevier Ltd
dc.rights.holderScopus
dc.subjectEucalyptus camaldulensis
dc.subjectLead
dc.subjectMembrane fluidity
dc.subjectOchrobactrum intermedium
dc.subjectPhytoremediation
dc.subjectRhizospheric bacteria
dc.titleIsolation and characterization of lead-tolerant Ochrobactrum intermedium and its role in enhancing lead accumulation by Eucalyptus camaldulensis
dc.typeArticle
mods.location.urlhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-80055079145&doi=10.1016%2fj.chemosphere.2011.06.086&partnerID=40&md5=3e6a26eacfd7e3c62946fde033485afe
oaire.citation.endPage590
oaire.citation.issue4
oaire.citation.startPage584
oaire.citation.volume85
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