Artocarpin-enriched extract reverses collagen metabolism in UV-exposed fibroblasts

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dc.contributor.authorKhwunjit Itsarasook
dc.contributor.authorKornkanok Ingkaninan
dc.contributor.authorJarupa Viyoch
dc.contributor.correspondenceJ. Viyoch; Department of Pharmaceutical Technology, Naresuan University, Phitsanulok, 65000, Thailand; email: jarupav@nu.ac.th
dc.date.accessioned2025-03-10T07:37:40Z
dc.date.available2025-03-10T07:37:40Z
dc.date.issued2014
dc.description.abstractIn previous studies, the Artocarpus incisus extract containing 45% w/w artocarpin showed activities of antioxidation, antimelanogenesis and restoration of wrinkled-skin fibroblasts. Here, extract containing 90% w/w artocarpin was tested for its antioxidant activity and in ultraviolet (UV) A-irradiated fibroblasts, its ability to restore type I collagen and inhibit matrix metalloproteinase-1 (MMP-1) elevation. This extract was a less effective antioxidant of EC50 of 116.0 ± 5.1 _g/mL than L-ascorbic acid (9.7 ± 0.01 _g/mL). The extract (0.625-50 _g/mL) showed no cytotoxicity toward primary human skin fibroblasts. MMP-1 was markedly elevated at 72 h after UVA irradiation compared to non-irradiation cells (p < 0.01). This UVA-induced elevation was inhibited by 50 _g/mL extract or 50 ng/mL all-trans retinoic acid. In an aged and sun-exposed skin tissue culture model, the increase of epidermal thickness in the 250 _g/mL artocarpin-enriched extract or 75 _g/mL all-trans retinoic acid-treated group when compared to the non-treated group was markedly observed since day 1 of treatment. Moreover, the extract or all-trans retinoic acid-treated groups exhibited higher density of immunofluorescence staining of type I collagen than non-treated group. This coincides with significantly higher (p < 0.05) collagen content, as indicated by measuring hydroxyproline. Our results firstly revealed that the artocarpin-enriched extract reversed the activities of UVA-irradiated fibroblasts and improved the type I collagen deposition in aged/photoaged skin. © 2014 Versita Warsaw and Springer-Verlag Wien.
dc.identifier.citationBiologia (Poland)
dc.identifier.doi10.2478/s11756-014-0394-4
dc.identifier.issn63088
dc.identifier.scopus2-s2.0-84906315244
dc.identifier.urihttps://repository.dusit.ac.th//handle/123456789/4959
dc.languageEnglish
dc.publisherVersita
dc.rightsAll Open Access; Hybrid Gold Open Access
dc.rights.holderScopus
dc.subjectartocarpin-enriched extract
dc.subjectArtocarpus incisus
dc.subjectcollagen metabolism
dc.subjectMMP-1 production
dc.subjecttype I procollagen production
dc.titleArtocarpin-enriched extract reverses collagen metabolism in UV-exposed fibroblasts
dc.typeArticle
mods.location.urlhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84906315244&doi=10.2478%2fs11756-014-0394-4&partnerID=40&md5=f25bd264fde494d61f162e3489a68055
oaire.citation.endPage951
oaire.citation.issue7
oaire.citation.startPage943
oaire.citation.volume69
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