Phosphoproteomic analysis of apoptotic hematopoietic stem cells from hemoglobin E/_-thalassemia

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dc.contributor.authorSaranyoo Ponnikorn
dc.contributor.authorTasanee Panichakul
dc.contributor.authorKitima Sresanga
dc.contributor.authorChokdee Wongborisuth
dc.contributor.authorSittiruk Roytrakul
dc.contributor.authorSuradej Hongeng
dc.contributor.authorSumalee Tungpradabkul
dc.contributor.correspondenceS. Hongeng; Department of Pediatrics, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand; email: rashe@mahidol.ac.th
dc.date.accessioned2025-03-10T07:37:41Z
dc.date.available2025-03-10T07:37:41Z
dc.date.issued2011
dc.description.abstractBackground: Hemoglobin E/_-thalassemia is particularly common in Southeast Asia and has variable symptoms ranging from mild to severe anemia. Previous investigations demonstrated the remarkable symptoms of _-thalassemia in terms of the acceleration of apoptotic cell death. Ineffective erythropoiesis has been studied in human hematopoietic stem cells, however the distinct apoptotic mechanism was unclear.Methods: The phosphoproteome of bone marrow HSCs/CD34+ cells from HbE/_-thalassemic patients was analyzed using IMAC phosphoprotein isolation followed by LC-MS/MS detection. Decyder MS software was used to quantitate differentially expressed proteins in 3 patients and 2 normal donors. The differentially expressed proteins from HSCs/CD34+ cells were compared with HbE/_-thalassemia and normal HSCs.Results: A significant change in abundance of 229 phosphoproteins was demonstrated. Importantly, the analysis of the candidate proteins revealed a high abundance of proteins that are commonly found in apoptotic cells including cytochrome C, caspase 6 and apoptosis inducing factors. Moreover, in the HSCs patients a significant increase was observed in a specific type of phosphoserine/threonine binding protein, which is known to act as an important signal mediator for the regulation of cell survival and apoptosis in HbE/_-thalassemia.Conclusions: Our study used a novel method to investigate proteins that influence a particular pathway in a given disease or physiological condition. Ultimately, phosphoproteome profiling in HbE/_-thalassemic stem cells is an effective method to further investigate the cell death mechanism of ineffective erythropoiesis in _-thalassemia. Our report provides a comprehensive phosphoproteome, an important resource for the study of ineffective erythropoiesis and developing therapies for HbE/_-thalassemia. © 2011 Ponnikorn et al; licensee BioMed Central Ltd.
dc.identifier.citationJournal of Translational Medicine
dc.identifier.doi10.1186/1479-5876-9-96
dc.identifier.issn14795876
dc.identifier.scopus2-s2.0-79959461089
dc.identifier.urihttps://repository.dusit.ac.th//handle/123456789/5022
dc.languageEnglish
dc.rightsAll Open Access; Gold Open Access; Green Open Access
dc.rights.holderScopus
dc.subjectApoptosis
dc.subjectHemoglobin E/_-thalassemia
dc.subjectHSCs/CD34<sup>+ </sup>
dc.subjectPhosphoproteome
dc.titlePhosphoproteomic analysis of apoptotic hematopoietic stem cells from hemoglobin E/_-thalassemia
dc.typeArticle
mods.location.urlhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-79959461089&doi=10.1186%2f1479-5876-9-96&partnerID=40&md5=ea24c7f47f053639c829a8ceb5607bcd
oaire.citation.issue1
oaire.citation.volume9
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