High Loading Degradation of Poly(lactide)/Thermoplastic Starch Blend Film Using Mixed-Enzymes Produced by Fed-Batch Culture of Laceyella sacchari LP175

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dc.contributor.authorThanasak Lomthong
dc.contributor.authorSrisuda Samaimai
dc.contributor.authorRangrong Yoksan
dc.contributor.authorSukhumaporn Krajangsang
dc.contributor.authorVichien Kitpreechavanich
dc.contributor.correspondenceV. Kitpreechavanich; Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand; email: fsciwck@ku.ac.th
dc.date.accessioned2025-03-10T07:35:06Z
dc.date.available2025-03-10T07:35:06Z
dc.date.issued2022
dc.description.abstractPurpose: Co-production of poly(l-lactide) (PLLA)-degrading enzyme and raw starch-degrading enzyme (RSDE) was investigated using a fed-batch culture of Laceyella sacchari LP175 in a 10.0ÊL airlift fermenter. Agricultural products were used as substrates for production of enzymes to degrade the poly(lactide)/thermoplastic starch blend film at high concentration. Methods: Fed-batch culture was performed in a 10.0ÊL airlift fermenter for co-production of PLLA-degrading enzyme and RSDE by L. sacchari LP175. Parameters affecting PLA/thermoplastic starch (TPS) blend film at high loading (100Êg/L) degradation were optimized using response surface methodology (RSM) with a central composite design (CCD) at 50Ê¡C for 24Êh. Results: Maximum enzyme production of PLLA-degrading enzyme and RSDE at 91.6 ± 7.21 and 120.1 ± 9.33 U/mL, respectively, were obtained when incubated at 50Ê¡C for 42Êh after adding raw cassava starch (3.34Êg/L) and PLA powder (0.52Êg/L) at 30Êh of cultivation. The optimum conditions for degradation (92.23%) from the model were enzyme concentration at 0.6% (w/v), time to add CaCO3 3Êh after digestion and 0.2ÊM of Tris-HCl buffer (pH 9.0) in the shaking flask scale with a 95% significance level (p < 0.05). These conditions gave the highest degradation at 90.65 ± 4.03% from the actual experimental. Scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR) revealed the mixed enzymes produced by L. sacchari LP175 hydrolyzed PLA/TPS blend film at 50Ê¡C. Conclusions: Results indicated the feasibility of producing mixed enzymes by L. sacchari LP175 and hydrolysis of PLA/TPS blend film at high concentration to reduce waste accumulation through biotechnological processes. Graphical Abstract: [Figure not available: see fulltext.] © 2021, The Author(s), under exclusive licence to Springer Nature B.V.
dc.identifier.citationWaste and Biomass Valorization
dc.identifier.doi10.1007/s12649-021-01644-2
dc.identifier.issn18772641
dc.identifier.scopus2-s2.0-85122695308
dc.identifier.urihttps://repository.dusit.ac.th//handle/123456789/4591
dc.languageEnglish
dc.publisherSpringer Science and Business Media B.V.
dc.rights.holderScopus
dc.subjectBiodegradation
dc.subjectCo-enzyme production
dc.subjectFed-batch culture
dc.subjectLaceyella sacchari LP175
dc.subjectPoly(lactide)/thermoplastic starch blend film
dc.titleHigh Loading Degradation of Poly(lactide)/Thermoplastic Starch Blend Film Using Mixed-Enzymes Produced by Fed-Batch Culture of Laceyella sacchari LP175
dc.typeArticle
mods.location.urlhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85122695308&doi=10.1007%2fs12649-021-01644-2&partnerID=40&md5=ffb1c7dd021d8d10042c8f2332961b8c
oaire.citation.endPage1991
oaire.citation.issue4
oaire.citation.startPage1981
oaire.citation.volume13
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