Browsing by Author "Woraphot Haritakun"

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    In vitro Antioxidant, Anti-Inflammatory, and Wound Healing Properties of Aloe Vera Infused with Canola Oil for Possible Application in Skin Cosmetics
    (Research and Development Institute Suan Dusit University, 2025) Khwunjit Itsarasook; Piyanuch Prompamorn; Jittarawadee Tanghiranrat; Amornrat Srisukong; Orapin Komutiban; Woraphot Haritakun; Kanlayaporn Chantree; K. Itsarasook; Department of Chemistry, Faculty of Science, Ramkhamhaeng University, Bangkok, 10240, Thailand; email: Khwunjit.its@hotmail.com
    This research investigates the antioxidant, anti-inflammatory, and wound-healing properties of Aloe vera oil, along with its cytotoxic effects on normal human dermal fibroblasts (NHDF), particularly in the context of its potential use as a natural antioxidant in skincare formulations. A. vera oil was extracted using an infusion method with canola oil, and its properties were thorough characterized. The concentration of aloin and cycloartenol in the A. vera oil were measured using high-performance liquid chromatography (HPLC). The resulted indicated that A. vera oil exhibited no cytotoxicity towards NHDF cells. Additionally, the oil demonstrated antioxidant activity, as evidenced by its ability to scavenge 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals, and its reducing potential assessed through free radical scavenging activity (FRAP) assays. Regarding its anti-inflammatory properties, the A. vera oil at a specific concentration showed no cytotoxic effects on RAW 264.7 macrophage cells. Notably, its potent anti-inflammatory effects significantly (p<0.05) reduced nitric oxide (NO) productions in RAW 264.7 macrophage cells stimulated by lipopolysaccharide (LPS), compared to the LPS-treated cells group. Moreover, NHDF cells treated with A. vera oil exhibited a statistically significant (p<0.05) improvement in wound closure area in a scratch wound assay, indicating enhanced cell migration. Collectively, these findings highlight the antioxidant, anti-inflammatory, and wound-healing properties of A. vera oil, underscoring its potential as an eco-friendly and beneficial ingredient for cosmetic skincare products. © 2025, Research and Development Institute Suan Dusit University. All rights reserved.
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    Preparation, Characterization and Evaluation of Octyl Methoxycinnamate (OMC)-Loaded Solid Lipid Nanoparticles (SLNs) by Using a Microemulsion Technique
    (Research and Development Institute Suan Dusit University, 2022) Khwunjit Itsarasook; Piyanuch Prompamorn; Surapa Modsuwan; Jittarawadee Tanghiranrat; Orapin Komutiban; Woraphot Haritakun; Jantharat Wutisatwongkul; Kanlayaporn Chantree; K. Itsarasook; Faculty of Science and Technology, Suphanburi Campus, Suan Dusit University, Suphanburi, 72000, Thailand; email: khwunjit_its@dusit.ac.th
    This research prepared the solid lipid nanoparticles (SLNs) based on microemulsion to load octyl methoxycinnamate (OMC) as a UV absorber for instability protection. The optimal microemulsion formulation was selected by visual assessment according to a transparent and thermodynamically system form. The optimal microemulsion obtained from the experiment composed of 10% w/w glyceryl monostearate (GMS, solid lipid), 10% w/w Tween 80 (surfactant), 15% w/w PEG-40 hydrogenated castor oil (surfactant) and 20% w/w ethanol (co-surfactant). Various processing parameters for the preparation of SLN was carried out using three factors including, 1) warm microemulsion (mL) and iced water ratio (mL), 2) homogenization speed (rpm) and 3) homogenization time (min) and two responses assessed were particle size and polydispersity index (PDI) to obtain a SLNs batch with smaller particle size and optimum PDI. The OMC-loaded SLN had 693.07± 0.05 nm mean particle size and 0.56±0.04 PDI, prepared by optimal processing parameters. The surface morphology of the SLNs was assessed by transmission electron microscopy (TEM), the result found that OMC-loaded SLN showed a smooth surface and spherical shape. Entrapment efficiency of OMC-loaded SLN was found to be 99.89±0.020%. The stability testing of the prepared SLN was studied. Result found that OMC-loaded SLN showed slightly lower stable compared with the initial condition. All obtained results indicated that this method and processing parameters can prepare the SLNs which was suitable for cosmetic application. © 2022, Research and Development Institute Suan Dusit University. All rights reserved.
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    Preparation, Characterization and Evaluation of Octyl Methoxycinnamate (OMC)-Loaded Solid Lipid Nanoparticles (SLNs) by Using a Microemulsion Technique
    (Graphicsite, 2023-09-26) Khwunjit Itsarasook; Piyanuch Prompamorn; Surapa Modsuwan; Jittarawadee Tanghiranrat; Orapin Komutiban; Woraphot Haritakun; Jantharat Wutisatwongkul; Kanlayaporn Chantree
    This research prepared the solid lipid nanoparticles (SLNs) based on microemulsion to load octyl methoxycinnamate (OMC) as a UV absorber for instability protection. The optimal microemulsion formulation was selected by visual assessment according to a transparent and thermodynamically system form. The optimal microemulsion obtained from the experiment composed of 10% w/w glyceryl monostearate (GMS, solid lipid), 10% w/w Tween 80 (surfactant), 15% w/w PEG-40 hydrogenated castor oil (surfactant) and 20% w/w ethanol (co-surfactant). Various processing parameters for the preparation of SLN was carried out using three factors including, 1) warm microemulsion (mL) and iced water ratio (mL), 2) homogenization speed (rpm) and 3) homogenization time (min) and two responses assessed were particle size and polydispersity index (PDI) to obtain a SLNs batch with smaller particle size and optimum PDI. The OMC-loaded SLN had 693.07± 0.05 nm mean particle size and 0.56±0.04 PDI, prepared by optimal processing parameters. The surface morphology of the SLNs was assessed by transmission electron microscopy (TEM), the result found that OMC-loaded SLN showed a smooth surface and spherical shape. Entrapment efficiency of OMC-loaded SLN was found to be 99.89±0.020% . The stability testing of the prepared SLN was studied. Result found that OMC-loaded SLN showed slightly lower stable compared with the initial condition. All obtained results indicated that this method and processing parameters can prepare the SLNs which was suitable for cosmetic application.
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    Skin Anti-Aging Potential of Ipomoea pes-caprae Ethanolic Extracts on Promoting Cell Proliferation and Collagen Production in Human Fibroblasts (CCD-986sk Cells)
    (MDPI, 2022) Tasanee Panichakul; Saranyoo Ponnikorn; Wipa Tupchiangmai; Woraphot Haritakun; Kitima Srisanga; T. Panichakul; Department of Cosmetic Science, Faculty of Science and Technology, Suan Dusit University, Bangkok, 228-228/1-3 Sirindhorn Rd. Bangphlat, 10700, Thailand; email: tasanee_pan@dusit.ac.th
    Collagen loss in the skin dermis is a major cause of age-related changes to the skin. Natural phytochemical substances are desirable for the prevention of skin aging and the formation of wrinkles. Ipomoea pes-caprae (IPC) has been utilized for nutritional and therapeutic purposes, and its extract contains collagenase inhibitory activity while causing no cytotoxicity. The purpose of this study was to examine the impact of IPC extracts on cell proliferation and collagen production in human fibroblasts (CCD-986sk cells). IPC leaves were macerated in 70% and 95% ethanol and the chemical composition of the resulting extracts (IPC70 and IPC95) were determined using high performance liquid chromatography (HPLC). The bioactivity of IPC extracts was examined in CCD-986sk cells, including antioxidant capacity, inhibition of collagenase, effects on cell proliferation and collagen production, as well as wound healing using an in vitro scratch test. Changes in expression of collagen type I (COL1A1), tumor growth factor beta 1 (TGFB1), and beta-fibroblast growth factor (FGF2) genes were also evaluated. The antioxidant and collagenase inhibitory properties of IPC extracts were associated with 3,5-di-caffeoylquinic acid, chlorogenic acid, and ferulic acid. IPC extracts at noncytotoxic concentrations significantly increased cell proliferation, collagen production, and wound healing. These effects appear linked to the upregulation of COL1A1, TGFB1, and FGF2 genes. The bioactivity of the IPC70 extract was greater than that for IPC95. This is useful in cosmeceutical applications for human skin aging. Our findings indicate that IPC extracts have the potential for use in skin anti-aging cosmeceutical preparations. © 2022 by the authors.
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    Stephapierrines A-H, new tetrahydroprotoberberine and aporphine alkaloids from the tubers of: Stephania pierrei Diels and their anti-cholinesterase activities
    (Royal Society of Chemistry, 2021) Waraluck Chaichompoo; Pornchai Rojsitthisak; Wachirachai Pabuprapap; Yuttana Siriwattanasathien; Pathumwadee Yotmanee; Woraphot Haritakun; Apichart Suksamrarn; P. Rojsitthisak; Department of Food and Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, 10330, Thailand; email: pornchai.r@chula.ac.th
    Eight new alkaloids, which are four new tetrahydroprotoberberine alkaloids, stephapierrines A-D (1-4), and four new aporphine alkaloids, stephapierrines E-H (5-8), together with three new naturally occurring alkaloids (9-11) and thirty-four known alkaloids (12-45) were isolated from the tubers of Stephania pierrei Diels. The structures of the new compounds were elucidated by spectroscopic analysis and physical properties. The structures of the known compounds were characterized by comparison of their spectroscopic data with those previously reported. Compound 42 exhibited the strongest acetylcholinesterase (AChE) inhibitory activity, which was more active than galanthamine, the reference drug. Compound 23 showed the highest butyrylcholinesterase (BuChE) inhibitory activity, which was also more active than galanthamine. Molecular docking studies are in good agreement with the experimental results. This journal is © The Royal Society of Chemistry.