Browsing by Author "Wipa Tupchiangmai"

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    Antioxidant Activity and Allelopathic Potential of Lippia nodiflora (L.) Michx on Germination and Growth of Neptunia javanica Miq. seeds
    (Research and Development Institute Suan Dusit University, 2020) Wipa Tupchiangmai; Wantana Mongkolvisut; W. Tupchiangmai; Department of Chemical Technology, Faculty of Science and Technology, Suan Dusit University, Bangkok, 10300, Thailand; email: wipa_tup@dusit.ac.th
    The research objective was to evaluate the antioxidant activity, the allelopathic potential on germination growth of Neptunia javanica Miq. seeds and the total phenolic content (TPC) of crude extracts and fractions from Lippia nodiflora (L.) Michx. In this research, the ethanol crude extracts of leaves (LE), stems (SE) and roots (RE) from L. nodiflora were subjected to study the antioxidant activity by using DPPH free radical scavenging assay and the allelopathic potential on germination seeds of N. javanica. The RE extract had the highest antioxidant activity with IC50 value of 136.87 ppm. The allelopathic effect of LE extract at a concentration of 20 mg/mL completely inhibited the seed germination of N. javanica. The LE fractions were separated by liquid-liquid partition with hexane and ethyl acetate system to afford the leaf hexane fraction (LH), leaf ethyl acetate fraction (LEtOAc) and leaf hydroethanolic fraction (LW). The antioxidant, TPC and allelopathic activities of all fractions were investigated. The antioxidant activity of LW (158.21) and LEtOAc (175.45) fractions were better than those of LE (296.87) extracts, while LH fraction showed the lowest antioxidant activity at 722.08 ppm. The active allelopathic compounds in LEtOAc at a concentration of 10 mg/mL showed complete inhibition of radicle and hypocotyl on germination seed growth and 50% of seed growth inhibition (IC50) was 3.14 mg/mL. Crude extracts and fractions showed more inhibited radicle growth than hypocotyl growth of N. javanica seeds. The TPC of leaves crude extracts and fractions were the highest. Interestingly, the total phenolic content of LEtOAc fraction, was the highest (0.072 mg GAE/g) that was related to the good allelopathic properties, compared with other fractions and crude extracts. Our results indicate that the ethanolic crude extracts and an ethyl acetate fraction of L. nodiflora leaves can be applied to weed control, especially for N. javanica. © 2020, Research and Development Institute Suan Dusit University. All rights reserved.
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    Antioxidant Activity and Allelopathic Potential of Lippia nodiflora (L.) Michx on Germination and Growth of Neptunia javanica Miq. seeds
    (Graphicsite, 2023-09-26) Wipa Tupchiangmai; Wantana Mongkolvisut
    The research objective was to evaluate the antioxidant activity, the allelopathic potential on germination growth of Neptunia javanica Miq. seeds and the total phenolic content (TPC) of crude extracts and fractions from Lippia nodiflora (L.) Michx. In this research, the ethanol crude extracts of leaves (LE), stems (SE) and roots (RE) from L. nodiflora were subjected to study the antioxidant activity by using DPPH free radical scavenging assay and the allelopathic potential on germination seeds of N. javanica. The RE extract had the highest antioxidant activity with IC50 value of 136.87 ppm. The allelopathic effect of LE extract at a concentration of 20 mg/mL completely inhibited the seed germination of N. javanica. The LE fractions were separated by liquid-liquid partition with hexane and ethyl acetate system to afford the leaf hexane fraction (LH), leaf ethyl acetate fraction (LEtOAc) and leaf hydroethanolic fraction (LW). The antioxidant, TPC and allelopathic activities of all fractions were investigated. The antioxidant activity of LW (158.21) and LEtOAc (175.45) fractions were better than those of LE (296.87) extracts, while LH fraction showed the lowest antioxidant activity at 722.08 ppm. The active allelopathic compounds in LEtOAc at a concentration of 10 mg/mL showed complete inhibition of radicle and hypocotyl on germination seed growth and 50% of seed growth inhibition (IC50) was 3.14 mg/mL. Crude extracts and fractions showed more inhibited radicle growth than hypocotyl growth of N. javanica seeds. The TPC of leaves crude extracts and fractions were the highest. Interestingly, the total phenolic content of LEtOAc fraction, was the highest (0.072 mg GAE/g) that was related to the good allelopathic properties, compared with other fractions and crude extracts. Our results indicate that the ethanolic crude extracts and an ethyl acetate fraction of L. nodiflora leaves can be applied to weed control, especially for N. javanica.
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    Skin Anti-Aging Potential of Ipomoea pes-caprae Ethanolic Extracts on Promoting Cell Proliferation and Collagen Production in Human Fibroblasts (CCD-986sk Cells)
    (MDPI, 2022) Tasanee Panichakul; Saranyoo Ponnikorn; Wipa Tupchiangmai; Woraphot Haritakun; Kitima Srisanga; T. Panichakul; Department of Cosmetic Science, Faculty of Science and Technology, Suan Dusit University, Bangkok, 228-228/1-3 Sirindhorn Rd. Bangphlat, 10700, Thailand; email: tasanee_pan@dusit.ac.th
    Collagen loss in the skin dermis is a major cause of age-related changes to the skin. Natural phytochemical substances are desirable for the prevention of skin aging and the formation of wrinkles. Ipomoea pes-caprae (IPC) has been utilized for nutritional and therapeutic purposes, and its extract contains collagenase inhibitory activity while causing no cytotoxicity. The purpose of this study was to examine the impact of IPC extracts on cell proliferation and collagen production in human fibroblasts (CCD-986sk cells). IPC leaves were macerated in 70% and 95% ethanol and the chemical composition of the resulting extracts (IPC70 and IPC95) were determined using high performance liquid chromatography (HPLC). The bioactivity of IPC extracts was examined in CCD-986sk cells, including antioxidant capacity, inhibition of collagenase, effects on cell proliferation and collagen production, as well as wound healing using an in vitro scratch test. Changes in expression of collagen type I (COL1A1), tumor growth factor beta 1 (TGFB1), and beta-fibroblast growth factor (FGF2) genes were also evaluated. The antioxidant and collagenase inhibitory properties of IPC extracts were associated with 3,5-di-caffeoylquinic acid, chlorogenic acid, and ferulic acid. IPC extracts at noncytotoxic concentrations significantly increased cell proliferation, collagen production, and wound healing. These effects appear linked to the upregulation of COL1A1, TGFB1, and FGF2 genes. The bioactivity of the IPC70 extract was greater than that for IPC95. This is useful in cosmeceutical applications for human skin aging. Our findings indicate that IPC extracts have the potential for use in skin anti-aging cosmeceutical preparations. © 2022 by the authors.