Browsing by Author "Thanyathep Sasisaowapak"
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Item Superior Auto-Identification of Trypanosome Parasites by Using a Hybrid Deep-Learning Model(Journal of Visualized Experiments, 2023) Veerayuth Kittichai; Morakot Kaewthamasorn; Suchansa Thanee; Thanyathep Sasisaowapak; Kaung Myat Naing; Rangsan Jomtarak; Teerawat Tongloy; Santhad Chuwongin; Siridech Boonsang; V. Kittichai; Faculty of Medicine, King MongkutÕs Institute of Technology Ladkrabang, Thailand; email: Veerayuth.ki@kmitl.ac.th; S. Boonsang; Department of Electrical Engineering, School of Engineering, King MongkutÕs Institute of Technology Ladkrabang, Thailand; email: siridech.bo@kmitl.ac.thTrypanosomiasis is a significant public health problem in several regions across the world, including South Asia and Southeast Asia. The identification of hotspot areas under active surveillance is a fundamental procedure for controlling disease transmission. Microscopic examination is a commonly used diagnostic method. It is, nevertheless, primarily reliant on skilled and experienced personnel. To address this issue, an artificial intelligence (AI) program was introduced that makes use of a hybrid deep learning technique of object identification and object classification neural network backbones on the in-house low-code AI platform (CiRA CORE). The program can identify and classify the protozoan trypanosome species, namely Trypanosoma cruzi, T. brucei, and T. evansi, from oil-immersion microscopic images. The AI program utilizes pattern recognition to observe and analyze multiple protozoa within a single blood sample and highlights the nucleus and kinetoplast of each parasite as specific characteristic features using an attention map. To assess the AI program's performance, two unique modules are created that provide a variety of statistical measures such as accuracy, recall, specificity, precision, F1 score, misclassification rate, receiver operating characteristics (ROC) curves, and precision versus recall (PR) curves. The assessment findings show that the AI algorithm is effective at identifying and categorizing parasites. By delivering a speedy, automated, and accurate screening tool, this technology has the potential to transform disease surveillance and control. It could also assist local officials in making more informed decisions on disease transmission-blocking strategies. © 2023 JoVE Journal of Visualized Experiments.