Browsing by Author "Thanasak Lomthong"

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    Antioxidant and antibacterial activities of Pandanus amaryllifolius Roxb. (Pandanaceae) prop roots and its application for a novel bacterial cellulose (Nata) fermentation by enzymatic hydrolysis
    (Open Science Publishers LLP Inc., 2022) Thanasak Lomthong; Manida Chorum; Srisuda Samaimai; Panarat Thongpoem; P. Thongpoem; Division of Biology, Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi, Thailand; email: panarat_t@rmutt.ac.th
    Pandanus amaryllifolius Roxb. (Pandanaceae) prop root was investigated for biological activities, i.e. antioxidant (DPPH radical scavenging assay) and antibacterial activity against Staphylococcus aureus DMST 2933 and Escherichia coli DMST 4212. The results showed that a crude extract of pandan prop roots exhibited antioxidant activity with IC50 of 230.24 ± 10.69 µg/ml, and it had a total phenolic content of 24.75 ± 0.74 mg GAE/g of TPC content and inhibited the growth of S. aureus DMST 2933 with 9.75 ± 0.35 mm of inhibition zone diameter. The prop root powder was used to develop a novel bacterial cellulose (BC) production using enzymatic hydrolysis. The maximum total soluble solids content at 2.67 ± 0.29 Brix was found when using prop root powder at 100 g/l with 4.0% (v/v) of the commercial enzyme (iKnowZyme¨ cellulase) after incubated at 50¡C, pH 5.0 for 24 h. The hydrolysis pandan prop root was fermented at room temperature for nine days with Komagataeibacter xylinus AGR 60, yielded 13.5 ± 0.50 mm of thickness with 7.90 ± 0.10 g of dry weight. Scanning electron microscope and Fourier-transform infrared spectroscopy were used to characterize the physical and chemical structure of the BC produced from pandan prop root, revealing that pandan prop root has the potential for a novel BC production with bioactivities of antioxidant and antibacterial properties. © 2022 Lomthong, et al.
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    Co-production of poly(l-lactide)-degrading enzyme and raw starch-degrading enzyme by Laceyella sacchari LP175 using agricultural products as substrate, and their efficiency on biodegradation of poly(l-lactide)/thermoplastic starch blend film
    (Elsevier Ltd, 2015) Thanasak Lomthong; Srisuda Hanphakphoom; Rangrong Yoksan; Vichien Kitpreechavanich
    The co-production of poly-(l-lactide)-degrading enzyme and raw starch-degrading enzyme by the thermophilic filamentous bacterium Laceyella sacchari LP175 in liquid medium using low-cost agricultural crops as substrates was investigated. Statistical mixture design experiments indicated that 5g of raw material - consisting of 2.35gL-1 cassava chips and 2.65gL-1 soybean meal in a suspension of 2.0gL-1 K2HPO4 and 1.0gL-1 KH2PO4 - gave the highest production of both enzymes when the culture was grown at 50�C for 24h cultivation. Addition of 1.0gL-1 of poly-(l-lactide) powder and 1.0gL-1 cassava starch to the medium increased poly-(l-lactide)-degrading enzyme and raw starch-degrading enzyme, respectively. Response surface methodology by central composite design found that the optimized concentration of 0.52gL-1 poly(l-lactide) powder and 3.34gL-1 cassava starch increased poly-(l-lactide)-degrading enzyme and raw starch-degrading enzyme activities up to 68.8UmL-1 and 86.1UmL-1, respectively. The 2% poly-(l-lactide)/thermoplastic starch (PLLA/TPS) blend (50:50) film was degraded up to 99.7% of weight loss by the crude enzyme at an initial pH of 9.0 for 4h. The high efficiency on biodegradation of poly-(l-lactide)/thermoplastic starch blend polymer by the obtained mixed enzymes from cheap and abundant agricultural products could be applied to reduce global environment from non-biodegradable materials. � 2015 Elsevier Ltd.
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    Degradation of poly(Butylene succinate) and poly(butylene succinate)/poly(lactide) blends using serine protease produced from laceyella sacchari LP175
    (Walailak University, 2021) Srisuda Samaimai; Sukhumaporn Krajangsang; Vichien Kitpreechavanich; Jednipit Borthong; Thanasak Lomthong; T. Lomthong; Division of Biology, Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi, Pathum Thani, 12110, Thailand; email: thanasak_l@rmutt.ac.th
    The thermophilic filamentous bacterium Laceyella sacchari LP175 was cultivated in a 10.0 L airlift fermenter to produce serine protease at 50 ¡C. Maximal serine protease activity at 1,123.32 ± 15.8 U/mL was obtained for cultivation at 0.6 vvm aeration rate for 36 h. The crude enzyme was applied for degradation of poly (butylene succinate) (PBS), and poly (butylene succinate)/poly(lactide) blend (PBS/PLA) powders at 50 ¡C for 48 h with different substrates and enzyme concentrations. Results showed that serine protease produced from L. sacchari LP175 degraded PBS and PBS/PLA at 46.5 ± 2.05 and 49.8 ± 1.45 %, respectively, at an initial substrate concentration of 100 g/L with 1,200 U/mL of serine protease activity. Percentage degradation of PBS and PBS/PLA was improved to 51.4 ± 1.06 and 56.9 ± 1.42 %, respectively, when upscaled in a 2.0 L stirrer fermenter with 200 rpm agitation rate. Degradation products evaluated by a scanning electron microscope (SEM) and Fourier transform infrared spectroscopy (FTIR) confirmed that serine protease produced from L. sacchari LP175 degraded both PBS and PBS/PLA polymers. Results showed that microbial enzyme technology could be used to degrade PBS and PBS/PLA blend polymers and reduce the accumulation of waste. © 2021, Walailak University. All rights reserved.
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    Development of sweet fermented rice (Khao-Mak) production using a pure culture of Amylomyces rouxii TISTR 3182 by the mixture design approach
    (Springer, 2024) Sujaya Rittisorn; Manida Chorum; Sorayaporn Ratchakit; Nattawan Klaeabangthong; Srisuda Samaimai; Thanasak Lomthong; T. Lomthong; Division of Biology, Faculty of Science and Technology, Rajamangala University of Technology, Thanyaburi, Pathum Thani, 12110, Thailand; email: thanasak_l@rmutt.ac.th
    A mixture design approach was used to investigate how different types of glutinous rice impacted the production of sweet fermented rice (Khao-Mak) using a pure culture of Amylomyces rouxii TISTR 3182. White glutinous rice provided the highest total soluble solids (TSS) and alcohol content (24.3 ± 0.35oBrix and 2.0 ± 0.14%, respectively). The combination of black glutinous rice (88%) with white glutinous rice (12%) gave the highest total phenolic content (TPC) at 120.21 ± 2.20ʵg GAE/mL. The optimal combination was chosen for large-scale production, yielding 22.00 ± 0.5oBrix, 1.63 ± 0.23%, and 102.98 ± 0.76ʵg GAE/mL of TSS, alcohol, and TPC, respectively. Thin-layer chromatography (TLC) revealed that the sugar obtained from the fermentation was glucose. Scanning electron microscopy (SEM) showed that growth of the fungal strain hydrolyzed the rice structure during fermentation. The crude extract of sweet fermented rice, fermented using a pure fungal strain culture through a biotechnological approach, suppressed the growth of Staphylococcus aureus DMST 2933 and Salmonella sp. ATCC 13311. © Association of Food Scientists & Technologists (India) 2024.
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    Enhancement of poly(L-lactide)-degrading enzyme production by Laceyella sacchari LP175 using agricultural crops as substrates and its degradation of poly(L-lactide) polymer
    (Elsevier Ltd, 2017) Thanasak Lomthong; Srisuda Hanphakphoom; Prachumporn Kongsaeree; Nantana Srisuk; Marie Guicherd; Gianluca Cioci; Sophie Duquesne; Alain Marty; Vichien Kitpreechavanich; V. Kitpreechavanich; Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand; email: fsciwck@ku.ac.th
    Optimization of the medium for poly(L-lactide) (PLLA)-degrading enzyme production in shake flask experiments was investigated using agricultural products as substrates. Cassava chips and soybean meal were the best carbon and nitrogen sources, respectively, as determined by the one-factor-at-a-time method. Enzyme production was significantly enhanced by the addition of phosphates, but was suppressed by the addition of ammonium salt. The maximum enzyme production, 65.5 U/mL, was obtained from the optimized medium consisting of 4.64 g/L cassava chips, 1.53 g/L soybean meal and 0.31 g/L PLLA powder using central composite design in the basal medium. The optimal physical factors in 3 L airlift fermenter were 50 �C, pH 7.0 and 0.5 vvm aeration rate for 18 h, yielded 94.4 U/mL. The crude enzyme was shown to be able to hydrolyze PLLA powder (91%) at 50 �C in 72 h which showed high efficiency for recycling of PLLA polymer and reducing the global environmental problem. � 2017 Elsevier Ltd
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    High Loading Degradation of Poly(lactide)/Thermoplastic Starch Blend Film Using Mixed-Enzymes Produced by Fed-Batch Culture of Laceyella sacchari LP175
    (Springer Science and Business Media B.V., 2022) Thanasak Lomthong; Srisuda Samaimai; Rangrong Yoksan; Sukhumaporn Krajangsang; Vichien Kitpreechavanich; V. Kitpreechavanich; Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand; email: fsciwck@ku.ac.th
    Purpose: Co-production of poly(l-lactide) (PLLA)-degrading enzyme and raw starch-degrading enzyme (RSDE) was investigated using a fed-batch culture of Laceyella sacchari LP175 in a 10.0ÊL airlift fermenter. Agricultural products were used as substrates for production of enzymes to degrade the poly(lactide)/thermoplastic starch blend film at high concentration. Methods: Fed-batch culture was performed in a 10.0ÊL airlift fermenter for co-production of PLLA-degrading enzyme and RSDE by L. sacchari LP175. Parameters affecting PLA/thermoplastic starch (TPS) blend film at high loading (100Êg/L) degradation were optimized using response surface methodology (RSM) with a central composite design (CCD) at 50Ê¡C for 24Êh. Results: Maximum enzyme production of PLLA-degrading enzyme and RSDE at 91.6 ± 7.21 and 120.1 ± 9.33 U/mL, respectively, were obtained when incubated at 50Ê¡C for 42Êh after adding raw cassava starch (3.34Êg/L) and PLA powder (0.52Êg/L) at 30Êh of cultivation. The optimum conditions for degradation (92.23%) from the model were enzyme concentration at 0.6% (w/v), time to add CaCO3 3Êh after digestion and 0.2ÊM of Tris-HCl buffer (pH 9.0) in the shaking flask scale with a 95% significance level (p < 0.05). These conditions gave the highest degradation at 90.65 ± 4.03% from the actual experimental. Scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR) revealed the mixed enzymes produced by L. sacchari LP175 hydrolyzed PLA/TPS blend film at 50Ê¡C. Conclusions: Results indicated the feasibility of producing mixed enzymes by L. sacchari LP175 and hydrolysis of PLA/TPS blend film at high concentration to reduce waste accumulation through biotechnological processes. Graphical Abstract: [Figure not available: see fulltext.] © 2021, The Author(s), under exclusive licence to Springer Nature B.V.
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    Production of poly (l-lactide)-degrading enzyme by Actinomadura keratinilytica strain T16-1 under solid state fermentation using agricultural wastes as substrate
    (Springer Science and Business Media Deutschland GmbH, 2021) Sukhumaporn Krajangsang; Nuttanisa Dechsresawut; Thanasak Lomthong; Srisuda Samaimai; S. Samaimai; Faculty of Science and Technology, Suan Dusit University, Bangkok, 10700, Thailand; email: srisuda_sam@dusit.ac.th
    Poly (l-lactide) (PLLA) is an aliphatic polyester that can be obtained from renewable resources and degraded by various microorganisms. In previous reports, Actinomadura keratinilytica strain T16-1 demonstrated high ability to degrade PLLA under various conditions. PLLA-degrading enzyme production under solid state fermentation has been sparsely studied. PLLA-degrading enzyme production by A. keratinilytica strain T16-1 was investigated using agricultural wastes as substrate under solid state fermentation (SSF). Three agricultural wastes as soybean meal, cassava chips and duckweed were tested as substrates for PLLA-degrading enzyme production by statistical methods using mixture design. Results revealed that using duckweed as the substrate gave the highest enzyme production (138.66 ± 13.57 U/g dry substrate). Maximum enzyme activity of 391.24 ± 15.57 U/g dry substrate was obtained under 10Êg duckweed, 10% inoculum size, 7Êdays of cultivation time, pH 7.0, 2.8% PLLA powder, and 60% moisture content at 45Ê¡C. It can be concluded that duckweed is an inexpensive substrate, which reduces the costs of PLLA-degrading enzyme production, as an alternative to effective water weed management. © 2021, King Abdulaziz City for Science and Technology.
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    Selection of Potential Bacteria in Termite Nest and Gut for Sustainable Agriculture
    (Walailak University, 2024) Phanukit Kunhachan; Wandee Sirithana; Orapin Komutiban; Vassanasak Limkhuansuwan; Phanchai Menchai; Chanaporn Trakunjae; Thanasak Lomthong; Kriangsak Ruchusatsawat; Srisuda Samaimai; S. Samaimai; Faculty of Science and Technology, Suan Dusit University, Bangkok, 10700, Thailand; email: srisuda_sam@dusit.ac.th
    In this study, bacteria with the best abilities in cellulose degradation, siderophore production, phosphate solubility, and Pythium parasitica inhibition were selected from termite nests and guts. The isolate BTNASP 5-2, BTPK 5-3 and BTNA 5-1 from termite guts exhibited highest in siderophore production index (SPI) (4.16 ± 0.21), phosphate solubilizing index (PSI) (2.10 ± 0.14) and percentage inhibition of radial growth (PIRG) (67.07 ± 4.02 %), respectively. The BGNACMC 4-3 isolated from termite nest gave the highest cellulolytic index (CI) of 5.17 ± 0.24. Bacterial classification was performed using 16s rRNA gene sequencing. The isolates BTPK 5-3, BGNACMC 4-3 and BTNA 5-1 were found closely related to Bacillus cereus, whereas the bacterial isolate BTNASP 5-2 was closely related to Bacillus subtilis. It is also suggested that the Bacillus cereus exhibited a variety of biological activities, denoting the highest cellulase, phosphate-solubilizing and antifungal activities, while Bacillus subtilis produced only a siderophore. The results obtained suggest that the bacteria selected will be used to develop bio-compost to promote plant growth, leading to sustainable farming. © 2024, Walailak University. All rights reserved.