Browsing by Author "Siri Chauin"

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    Histological type of intrahepatic cholangiocarcinoma differentiated by genetic alteration from AP-PCR fingerprint
    (Asian Pacific Organization for Cancer Prevention, 2011) Ubol Chuensumran; Pensri Saelee; Sopit Wongkham; Chawalit Pairojkul; Siri Chauin; Songsak Petmitr; U. Chuensumran; Department of Chemistry, Faculty of Science and Technology, Rajabhat University Suan Dusit, Bangkok, Thailand; email: ubolc@yahoo.com
    Cholangiocarcinoma (CCA), the malignant neoplasm of the biliary epithelium, is usually fatal due to difficulty in early diagnosis and lack of availability of effective therapy. The genetic mechanisms involved in the development of CCA are not well understood and only a few cytogenetic studies have been published. In this study, genomic instability in 30 Thai cases of intrahepatic cholangiocarcinoma (ICC) was assessed using an arbitrarily primed- polymerase chain reaction (AP-PCR) method. Genetic alterations were analyzed as banding pattern changes between tumors and corresponding normal DNA. The abnormal band present at the highest frequency (23/30 cases, 77%) appeared with the AO16 primer. Statistical analysis also showed that DNA alteration from this primer was significantly associated with the moderately to poorly differentiated histological type (P = 0.038). Kaplan-Meier survival curves showed borderline significance for this DNA aberration (P = 0.06 by the log-rank test). This DNA fragment may thus be of use to predict degree of malignancy of the disease.
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    Ubiquitin-specific protease 14 expression associated with intrahepatic cholangiocarcinoma cell differentiation
    (Asian Pacific Organization for Cancer Prevention, 2011) Ubol Chuensumran; Pensri Saelee; Phaibul Punyarit; Sopit Wongkham; Chawalit Pairojkul; Siri Chauin; Songsak Petmitr; U. Chuensumran; Department of Chemistry, Faculty of Science and Technology, Rajabhat University Suan Dusit, Thailand; email: ubol_c@yahoo.com
    The purpose of this study was to identify the gene alterations amplified from AO16 primer and examine whether the expression patterns of USP14 in clinical specimens from patients with intrahepatic cholangiocarcinoma (ICC) is associated with cancer cells. DNA from tumor and corresponding normal tissues of 52 patients was amplified with 33 arbitrary primers. The DNA fragment that altered most frequently in ICC was cloned, sequenced, and identified by comparison with known nucleotide sequences in the genome database. The DNA copy numbers of the allelic alterations in cholangiocarcinoma were determined by quantitative real-time PCR and interpreted as allelic loss or DNA amplification by comparison with the reference gene. Associations between allelic imbalance and clinicopathological parameters of ICC patients were evaluated by _2-tests. The Kaplan-Meier method was used to analyze survival rates. Immunohistochemically, USP14 showed weak cytoplasmic staining in normal bile duct epithelial cells. It was strongly detected in 21 cancer patients (43.8%). There were correlations between USP14 expression level and the clinicopathological features of ICC, histological grade (P < 0.05). However, there were no significant differences in age, gender, tumor size, metastasis, lymph node metastasis, and staging. USP14 expression was related to cholangiocarcinoma cell differentiation. Due to their emerging role in control of multiple signaling pathways and oncoproteins, USP14 inhibitors may be useful for anticancer agents.