Browsing by Author "Savitri Vatanyoopaisarn"

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    Dyadobacter and Sphingobacterium isolated from herbivore manure in Thailand and their cellulolytic activity in various organic waste substrates
    (Kasetsart University, 2019) Yuwarad Photphisutthiphong; Savitri Vatanyoopaisarn; Y. Photphisutthiphong; Program in of Environmental Technology, Faculty of Science and Technology, Suan Dusit University, Bangkok, 10700, Thailand; email: muayyai@hotmail.com
    Nineteen isolates of carboxy methyl cellulose (CMC) degrading bacteria were screened from various herbivore manures. The strain V5 and W2, isolated from elephant and cow manures, were the top two isolates that produces high cellulase activity of 0.16�0.005 and 0.14�0.0043 U/mL, respectively. Study of nitrogen sources showed that 0.6% yeast extract and 0.8% peptone were favorable for V5 and W2, respectively, to maximize their cellulase activity. Different organic wastes for example rice straw, bagasse, grass and garland, were also applied as carbon sources for cellulase enzyme induction of the isolates. The two isolates (V5 and W2) possess 2-fold higher cellulase activity, when grown in the presence of garland waste (maximum at 0.60�0.0086 U/mL), than the other organic wastes. The molecular identification of the V5 and W2 isolates based on 16S rDNA sequencing analysis appeared in the genus Dyadobacter and Sphingobacterium, respectively. � 2019 Elsevier B.V.. All rights reserved.
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    The production of bacterial cellulose from organic low-grade rice
    (Enviro Research Publishers, 2020) Yuwarat Photphisutthiphong; Savitri Vatanyoopaisarn; Y. Photphisutthiphong; Department of Environmental Technology, Faculty of Science and Technology, Suan Dusit University, Bangkok, 10700, Thailand; email: muayyai@hotmail.com
    Bacterial cellulose (BC) is a dietary fiber produced from some strains of acetic acid bacteria. Coconut water is the major substrate to ferment the bacterial cellulose for the food industry. However, the area of plantation is limited in a specific region, so the shortage of raw material occasionally occurred from the natural disaster. In contrast, rice cultivation is a major crop, millions of tons produced yearly. The rice mill industry also yields 2.5% of the underspecification grains. This research demonstrated the use of organic low-grade rice strains (Riceberry rice, brown jasmine rice, jasmine rice, red jasmine rice, and Homnil rice) as substrates for BC production. The study showed that the ground rice powder required enzymatic hydrolysis of 25 _l of alpha-amylase 10%(v/v) at 90¡C for 30 min, and followed by 50 _l of glucoamylase 20% (v/v) at 60¡C for 90 minutes to completely convert starch to reducing sugar. Such enzymatic hydrolysate was in liquid form with TSS of 8¡Brix. The cellulose production by Komagatacibacter nataicola TISTR 975 was carried out using the saccharified rice medium containing 0.7% (NH4)2SO4, initial pH 5.0 with 15% inoculum. Incubation at a static state for 15 days yielded the maximum weight of bacterial cellulose at 401.717 ±5.383 g/l when brown jasmine rice was the substrate. For jasmine rice, red jasmine rice, Homnil, and Riceberry rice, the weights of cellulose were 367.294 ±3.986, 335.244 ±4.707, 321.233 ±4.279 and 308.100 ±2.809g/l, respectively. Sensory scores of the cellulose product showed that the cellulose in the syrup was highly accepted. Different cubic sizes and quantities were prepared and mixed in 3 food products. A rice beverage the size of 0.5_0.5_0.5 cm3 at 15% (w/v) received the highest attribute scores. Whereas in soy yogurt, 1.0_1.0_1.0 cm3 at 10% (w/v) the mix of cellulose was the most preferable. The herbal drink assorted with bacterial cellulose of 0.25_0.25_0.25 cm3 at 20% (w/v) had the highest scores out of 30 panelists. © 2020 The Author(s). Published by Enviro Research Publisher.