Browsing by Author "Rungnapa Tangchitcharoenkhul"

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    An evaluation of educational provision quality at graduate level of rajabhat universities: Multi-level analysis
    (Canadian Center of Science and Education, 2012) Rungnapa Tangchitcharoenkhul; Nonglak Wiratchai; R. Tangchitcharoenkhul; Suan Dusit Rajabhat University, Dusit, Bangkok 10300, Ratchasima Road 295, Thailand; email: atrungnapa@yahoo.com
    The purposes of this research were 1) to analyze the educational provision quality scores at graduate level of Rajabhat universities by programs and to analyze the guideline for improving no quality programs at graduate level of Rajabhat universities using Data Envelopment Analysis (DEA) and 2) to analyze 2 level variables (micro level and macro level) that affected to the educational provision quality at graduate level of Rajabhat universities using multi-level analysis. The DEA and multi-level analysis collected by questionnaires from 1,608 of 5 groups of stakeholders (administrators, faculty members, students, alumni and employers). The estimated educational provision quality scores of 27 programs ranged from 45.52-100.00%. The numbers of educational provision quality programs were 11, 9, 10, 9 and 7 evaluating from administrators, faculty members, students, alumni and employers. Of those improving no quality programs, they were increased outputs/outcomes; percentage of a student who graduates within period of time, numbers of published of faculty members' researches, numbers of alumni award, numbers of utilization of faculty members' researches and value-added of students in order to enhance their performance to increase their quality. The result of multi-level analysis indicated that factors explaining the quality of programs were numbers of university campus, administrator academic leadership and Office of the Higher Education Commission monitoring.
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    Antibacterial, antioxidant, cytotoxic effects and GC-MS analysis of mangrove-derived Streptomyces achromogenes TCH4 extract
    (Institut za Bioloska Istrazivanja, 2021) Janpen Tangjitjaroenkun; Wanchai Pluempanupat; Rungnapa Tangchitcharoenkhul; Waraporn Yahayo; Roongtawan Supabphol; J. Tangjitjaroenkun; Department of Resources and Environment, Faculty of Science at Si Racha, Kasetsart, University, Chonburi, Thailand; email: janpen.ta@ku.th; R. Supabphol; Department of Physiology, Faculty of Medicine, Srinakharinwirot University, Bangkok, Thailand; email: roongtawans@gmail.com
    The in vitro biological activities of the ethyl acetate extract of the culture filtrate from Streptomyces achromogenes TCH4 (TCH4 extract) were evaluated. The ethyl acetate extract of TCH4 produced a bacteriostatic effect against Enterobacter cloacae, Staphylococcus aureus, Staphylococcus saprophyticus, Bacillus subtilis, methicillin-resistant S. aureus (MRSA) and Klebsiella pneumoniae. The extract had bactericidal activity against S. aureus, S. saprophyticus, S. aureus (MRSA) and K. pneumoniae with minimum bactericidal concentration (MBC) values in the range of 500-1000 µg/mL. The total phenolic and flavonoid contents in TCH4 extract were 107.20±2.57 mg gallic acid equivalent (GAE)/g and 44.91±0.84 mg quercetin equivalent (QE)/g of dry extract. Antioxidant activity was determined by DPPH radical (IC50 299.64±6.83 µg/mL) and ABTS radical scavenging (IC50 65.53±0.95 _g/mL), and the ferric-reducing antioxidant power (FRAP) (822.76±9.12 mM FeSO4.7H2O/g dry extract) assays. TCH4 extract exhibited cytotoxic activity in the DU-145 cell line (IC50 9.36±0.37 _g/mL). Analysis of extract constituents by GC-MS revealed pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl) (36.85%), benzeneacetamide (23.76%), and deferoxamine (12.85%) as the major compounds, which have been reported to possess pharmaceutical properties. S. achromogenes TCH4 could be a potential source of bioactive metabolites with antibacterial, antioxidant and anticancer activities for pharmaceutical applications. © 2021 by the Serbian Biological Society
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    Antioxidant properties of the extract from culture filtrate of schizophyllum commune
    (Research Journal of Pharmacy and Technology, 2020) Janpen Tangjitjaroenkun; Rungnapa Tangchitcharoenkhul; J. Tangjitjaroenkun; Department of Resources and Environment, Faculty of Science at Sriracha, Kasetsart University, Chonburi, Sriracha Campus, Thailand; email: xjanpen@gmail.com
    Schizophyllum commune mushroom is a wood decay fungus, widely consumed in traditional food and medicine. The study aimed to investigate the antioxidant activity, total phenolic and flavonoid content in the extract from culture filtrate of S. commune. Results showed that the total phenolic content present in the extract was found to be 672.17±9.55mg gallic acid equivalent (GAE)/g dry extract and total flavonoid content was 211.52±4.01mg quercetin equivalent (QAE)/g dry extract. The extract from culture filtrate of S. commune showed the highest free radical scavenging activity with IC50 values of 6.88±0.41 and 1.58±0.08µg/mL against DPPH and ABTS radicals, respectively. In FRAP assay, the extract showed high activity of 17,328.23±310.72mM Fe (II) equivalent/g dry extract. These results suggested that the extract from culture filtrate of S. commune can be used as a source of natural antioxidants and dietary supplements. © RJPT All right reserved.
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    Chemical compositions of essential oils of Amomum verum and Cinnamomum parthenoxylon and their in vitro biological properties
    (Nickan Research Institute, 2020) Janpen Tangjitjaroenkun; Rungnapa Tangchitcharoenkhul; Waraporn Yahayo; Suangson Supabphol; Ruengrit Sappapan; Roongtawan Supabphol; J. Tangjitjaroenkun; Department of Resources and Environment, Faculty of Science at Si Racha, Kasetsart University, Si Racha campus, Thailand; email: xjanpen@gmail.com
    Introduction: In eastern Thailand, Amomum verum and Cinnamomum parthenoxylon are native plants used by local communities for their medical and culinary properties. This study determined the chemical composition and biological activities of the essential oils from A. verum shoots (AVS-EO) and C. parthenoxylon wood (CPW-EO). Methods: Essential oils were extracted using hydro-distillation and analyzed using gas chromatography-mass spectroscopy (GC-MS). The antimicrobial activity was evaluated using the disc diffusion method and broth microdilution assay. The cytotoxic activity of the essential oils was assessed against the human prostate adenocarcinoma (DU145) cell line using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The antioxidant activity of the essential oils was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2_-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assays. The expression of antioxidant genes in the DU145 cells was evaluated using quantitative real-time polymerase chain reaction (qRT-PCR). Results: 1,8-Cineole was the main component in AVS-EO and CPW-EO with 84.38, and 45.65 %, respectively. AVS-EO had stronger antimicrobial activity than CPW-EO. The lowest minimum inhibitory concentration (MIC) and minimum microbicidal concentration (MMC) values of AVS-EO against Candida albicans were 0.3125 and 2.5 mg/mL, respectively. Both essential oils had time-dependent and dose-dependent cytotoxic effects on the DU145 human prostate adenocarcinoma cells. CPW-EO had high antioxidant activity toward DPPH and ABTS radicals with IC50 values of 4.528 ± 0.233 and 0.045 ± 0.007 mg/mL, respectively. The two essential oils up-regulated glutathione peroxidase (GPx) and glutathione reductase (GRx) mRNA expression in the oxidative stress response of DU145 cells. Conclusion: AVS-EO and CPW-EO might be added as natural ingredients in food or dietary supplement products for the benefit of microbial and prostate cancer inhibition. © 2020 Nickan Research Institute. All rights reserved.
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    In vitro antimicrobial and cytotoxic activities of mangrove actinomycetes from Eastern Thailand
    (Chiang Mai University, 2017) Janpen Tangjitjaroenkun; Rungnapa Tangchitcharoenkhul; Waraporn Yahayo; Roongtawan Supabphol; R. Supabphol; Department of Physiology, Faculty of Medicine, Srinakarinwirot University, Bangkok, 10110, Thailand; email: roongs@swu.ac.th
    Actinomycetes provide various kinds of secondary metabolites, including antibiotics and other therapeutic compounds. This study examines actinomycetes isolated from mangrove soil samples from eastern Thailand, Chonburi and Chantaburi provinces. From a total of 44 different actinomycete colonies, 35 isolates showed antimicrobial activity against one or more test organisms. In addition, 12 from 35 isolates exhibited potent antimicrobial activity against test microbes with a broad spectrum of activity. Fermentation time course studies of these 12 isolates, three strains (LC1, TCH4 and SCH2) revealed a potent antimicrobial activity in comparison to others. Thus they were chosen for further cell-based study to explore the cytotoxic, morphological, physiological, biochemical and molecular characteristics. The cytotoxic assay showed the possibility of these three strains for anticancer activity with an IC50 lower than 1%v/v. The nucleotide sequences of the 16S rDNA gene of the three strains matched with that of Streptomyces species in the morphological, physiological and biochemical characters. The findings of the present study demonstrate the potential of three mangrove actinomycetes in eastern Thailand to become a source of antimicrobial and anti-cancer metabolites. � 2017, Chiang Mai University. All rights reserved.
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    Zinc-Solubilizing Streptomyces spp. as Bioinoculants for Promoting the Growth of Soybean (Glycine max (L.) Merrill)
    (Korean Society for Microbiolog and Biotechnology, 2022) Chanwit Suriyachadkun; Orawan Chunhachart; Moltira Srithaworn; Rungnapa Tangchitcharoenkhul; Janpen Tangjitjareonkun; J. Tangjitjareonkun; Department of Resources and Environment, Faculty of Science at Sriracha, Kasetsart University, Chonburi, Sriracha Campus, 20230, Thailand; email: janpen.ta@ku.th
    Zinc-solubilizing bacteria can convert the insoluble form of zinc into soluble forms available to plants. This study was conducted to isolate and screen zinc-solubilizing actinobacteria from rhizosphere soils and to assess their effect on vegetable soybean growth. In total, 200 actinobacteria strains belonging to 10 genera were isolated from rhizosphere soil samples. Among these isolates, four showed zinc solubilization with solubilizing index values ranging from 3.11 to 3.78 on Bunt and Rovira agar supplemented with 0.1% zinc oxide. For the quantitative assay, in broth culture, strains CME34 and EX51 solubilized maximum available zinc contents of 529.71 and 243.58 _g/ml. Furthermore, indole-3-acetic acid (IAA) and ammonia were produced by these two strains, the strain CME34 produced the highest amount of IAA 4.62 _g/ml and the strain EX51 produced the highest amount of ammonia 361.04 _g/ml. In addition, the phosphate-solubilizing abilities in Pikovskaya's medium of CME34 and EX51 were 64.67 and 115.67 _g/ml. Based on morphological and biochemical characterization and 16S rDNA sequencing, the strains CME34 and EX51 were closely related to the genus Streptomyces. In a greenhouse experiment, single-strain inoculation of Streptomyces sp. CME34 or EX51 significantly increased the shoot length, root length, plant dry weight, number of pods per plant and number of seeds per plant of vegetable soybean plants compared to the uninoculated control. These findings facilitated the conclusion that the two Streptomyces strains have potential as zinc solubilizers and can be suggested as bioinoculants to promote the growth and yield of soybean. © 2022 by the authors.
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    Zinc-Solubilizing Streptomyces spp. as Bioinoculants for Promoting the Growth of Soybean (Glycine max (L.) Merrill)
    (Journal of Microbiology and Biotechnology, 2022-10) Rungnapa Tangchitcharoenkhul
    Zinc-solubilizing bacteria can convert the insoluble form of zinc into soluble forms available to plants. This study was conducted to isolate and screen zinc-solubilizing actinobacteria from rhizosphere soils and to assess their effect on vegetable soybean growth. In total, 200 actinobacteria strains belonging to 10 genera were isolated from rhizosphere soil samples. Among these isolates, four showed zinc solubilization with solubilizing index values ranging from 3.11 to 3.78 on Bunt and Rovira agar supplemented with 0.1% zinc oxide. For the quantitative assay, in broth culture, strains CME34 and EX51 solubilized maximum available zinc contents of 529.71 and 243.58 μg/ml. Furthermore, indole-3-acetic acid (IAA) and ammonia were produced by these two strains, the strain CME34 produced the highest amount of IAA 4.62 μg/ml and the strain EX51 produced the highest amount of ammonia 361.04 μg/ml. In addition, the phosphate-solubilizing abilities in Pikovskaya’s medium of CME34 and EX51 were 64.67 and 115.67 μg/ml. Based on morphological and biochemical characterization and 16S rDNA sequencing, the strains CME34 and EX51 were closely related to the genus Streptomyces. In a greenhouse experiment, single-strain inoculation of Streptomyces sp. CME34 or EX51 significantly increased the shoot length, root length, plant dry weight, number of pods per plant and number of seeds per plant of vegetable soybean plants compared to the uninoculated control. These findings facilitated the conclusion that the two Streptomyces strains have potential as zinc solubilizers and can be suggested as bioinoculants to promote the growth and yield of soybean.