Browsing by Author "Roongtawan Supabphol"
Now showing 1 - 3 of 3
Results Per Page
Sort Options
Item Antibacterial, antioxidant, cytotoxic effects and GC-MS analysis of mangrove-derived Streptomyces achromogenes TCH4 extract(Institut za Bioloska Istrazivanja, 2021) Janpen Tangjitjaroenkun; Wanchai Pluempanupat; Rungnapa Tangchitcharoenkhul; Waraporn Yahayo; Roongtawan Supabphol; J. Tangjitjaroenkun; Department of Resources and Environment, Faculty of Science at Si Racha, Kasetsart, University, Chonburi, Thailand; email: janpen.ta@ku.th; R. Supabphol; Department of Physiology, Faculty of Medicine, Srinakharinwirot University, Bangkok, Thailand; email: roongtawans@gmail.comThe in vitro biological activities of the ethyl acetate extract of the culture filtrate from Streptomyces achromogenes TCH4 (TCH4 extract) were evaluated. The ethyl acetate extract of TCH4 produced a bacteriostatic effect against Enterobacter cloacae, Staphylococcus aureus, Staphylococcus saprophyticus, Bacillus subtilis, methicillin-resistant S. aureus (MRSA) and Klebsiella pneumoniae. The extract had bactericidal activity against S. aureus, S. saprophyticus, S. aureus (MRSA) and K. pneumoniae with minimum bactericidal concentration (MBC) values in the range of 500-1000 µg/mL. The total phenolic and flavonoid contents in TCH4 extract were 107.20±2.57 mg gallic acid equivalent (GAE)/g and 44.91±0.84 mg quercetin equivalent (QE)/g of dry extract. Antioxidant activity was determined by DPPH radical (IC50 299.64±6.83 µg/mL) and ABTS radical scavenging (IC50 65.53±0.95 _g/mL), and the ferric-reducing antioxidant power (FRAP) (822.76±9.12 mM FeSO4.7H2O/g dry extract) assays. TCH4 extract exhibited cytotoxic activity in the DU-145 cell line (IC50 9.36±0.37 _g/mL). Analysis of extract constituents by GC-MS revealed pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl) (36.85%), benzeneacetamide (23.76%), and deferoxamine (12.85%) as the major compounds, which have been reported to possess pharmaceutical properties. S. achromogenes TCH4 could be a potential source of bioactive metabolites with antibacterial, antioxidant and anticancer activities for pharmaceutical applications. © 2021 by the Serbian Biological SocietyItem Chemical compositions of essential oils of Amomum verum and Cinnamomum parthenoxylon and their in vitro biological properties(Nickan Research Institute, 2020) Janpen Tangjitjaroenkun; Rungnapa Tangchitcharoenkhul; Waraporn Yahayo; Suangson Supabphol; Ruengrit Sappapan; Roongtawan Supabphol; J. Tangjitjaroenkun; Department of Resources and Environment, Faculty of Science at Si Racha, Kasetsart University, Si Racha campus, Thailand; email: xjanpen@gmail.comIntroduction: In eastern Thailand, Amomum verum and Cinnamomum parthenoxylon are native plants used by local communities for their medical and culinary properties. This study determined the chemical composition and biological activities of the essential oils from A. verum shoots (AVS-EO) and C. parthenoxylon wood (CPW-EO). Methods: Essential oils were extracted using hydro-distillation and analyzed using gas chromatography-mass spectroscopy (GC-MS). The antimicrobial activity was evaluated using the disc diffusion method and broth microdilution assay. The cytotoxic activity of the essential oils was assessed against the human prostate adenocarcinoma (DU145) cell line using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The antioxidant activity of the essential oils was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2_-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assays. The expression of antioxidant genes in the DU145 cells was evaluated using quantitative real-time polymerase chain reaction (qRT-PCR). Results: 1,8-Cineole was the main component in AVS-EO and CPW-EO with 84.38, and 45.65 %, respectively. AVS-EO had stronger antimicrobial activity than CPW-EO. The lowest minimum inhibitory concentration (MIC) and minimum microbicidal concentration (MMC) values of AVS-EO against Candida albicans were 0.3125 and 2.5 mg/mL, respectively. Both essential oils had time-dependent and dose-dependent cytotoxic effects on the DU145 human prostate adenocarcinoma cells. CPW-EO had high antioxidant activity toward DPPH and ABTS radicals with IC50 values of 4.528 ± 0.233 and 0.045 ± 0.007 mg/mL, respectively. The two essential oils up-regulated glutathione peroxidase (GPx) and glutathione reductase (GRx) mRNA expression in the oxidative stress response of DU145 cells. Conclusion: AVS-EO and CPW-EO might be added as natural ingredients in food or dietary supplement products for the benefit of microbial and prostate cancer inhibition. © 2020 Nickan Research Institute. All rights reserved.Item In vitro antimicrobial and cytotoxic activities of mangrove actinomycetes from Eastern Thailand(Chiang Mai University, 2017) Janpen Tangjitjaroenkun; Rungnapa Tangchitcharoenkhul; Waraporn Yahayo; Roongtawan Supabphol; R. Supabphol; Department of Physiology, Faculty of Medicine, Srinakarinwirot University, Bangkok, 10110, Thailand; email: roongs@swu.ac.thActinomycetes provide various kinds of secondary metabolites, including antibiotics and other therapeutic compounds. This study examines actinomycetes isolated from mangrove soil samples from eastern Thailand, Chonburi and Chantaburi provinces. From a total of 44 different actinomycete colonies, 35 isolates showed antimicrobial activity against one or more test organisms. In addition, 12 from 35 isolates exhibited potent antimicrobial activity against test microbes with a broad spectrum of activity. Fermentation time course studies of these 12 isolates, three strains (LC1, TCH4 and SCH2) revealed a potent antimicrobial activity in comparison to others. Thus they were chosen for further cell-based study to explore the cytotoxic, morphological, physiological, biochemical and molecular characteristics. The cytotoxic assay showed the possibility of these three strains for anticancer activity with an IC50 lower than 1%v/v. The nucleotide sequences of the 16S rDNA gene of the three strains matched with that of Streptomyces species in the morphological, physiological and biochemical characters. The findings of the present study demonstrate the potential of three mangrove actinomycetes in eastern Thailand to become a source of antimicrobial and anti-cancer metabolites. � 2017, Chiang Mai University. All rights reserved.