Browsing by Author "Rittipun Rungruang"

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    Additive effect of a combination of artocarpus lakoocha and glycyrrhiza glabra extracts on tyrosinase inhibition in melanoma B16 cells
    (MDPI AG, 2020) Tasanee Panichakul; Teerapat Rodboon; Prasit Suwannalert; Chanchai Tripetch; Rittipun Rungruang; Nattaporn Boohuad; Piyawan Youdee; T. Panichakul; Department of Cosmetic Science, Faculty of Science and Technology, Suan Dusit University, Bangkok, 228-228/1-3 Sirindhorn Rd. Bangplad, 10700, Thailand; email: tasanee_pan@dusit.ac.th
    Artocarpus lakoocha (Al) and Glycyrrhiza glabra (Gg) extracts have been reported to show tyrosinase inhibitory activity and melanin pigment reduction. This is the first study to assess the combination of Al and Gg extracts in enhancing inhibition of tyrosinase and reduction of melanin pigments. Al and Gg extracted by maceration in 70% and 95% ethanol were analyzed for oxyresveratrol and glabridin using Ultra High Performance Liquid Chromatography. Extracts of Al and Gg singly and combinations of Al95 and Gg95 were tested for cytotoxicity, tyrosinase inhibitory activity, and reduction of melanin pigments in melanoma B16 cells. Al95 had higher antioxidant, tyrosinase inhibitory activity and reduced more melanin pigments in B16 cells compared to Al 70, and exhibited higher levels of oxyresveratrol. Gg95 inhibited oxidative stress and mushroom tyrosinase better than Gg70, and exhibited higher levels of glabridin. Combinations of Al95 and Gg95 at various ratios (concentration of 0.1 mg/mL) were not cytotoxic to B16 cells. Interestingly, Al95 and Gg95 combined at a ratio 9:1 reduced melanin pigment up to 53% in B16 cells. This combination of Al95 and Gg95 extracts exhibited the additive effect of reducing melanin pigments by suppressing the expression of microphthalmia-associated transcription factor (MITF), tyrosinase (TYR) and tyrosinase-related protein-2 (TRP-2) in B16 cells. The combination of Al and Gg extracts could be developed as skin care products for hyperpigmentation treatment. © 2020 by the authors.
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    Antimicrobial Effect of Deodorant Products Containing Rhinacanthus nasutus Extract for Reducing Armpit Odor
    (Research and Development Institute Suan Dusit University, 2022) Nartlada Onvimol; Rittipun Rungruang; Surapha Modsuwan; Tasanee Panichakul; T. Panichakul; Faculty of Science and Technology, Suan Dusit University, Bangkok, 10700, Thailand; email: tasanee_p@yahoo.com
    The aim of this study was to investigate the effectiveness of developing a roll-on deodorant that can reduce armpit microbiota bacteria to decrease armpit odor. The richest apocrine sweat glands in the armpit region secrete a variety of odor precursors that are transformed into volatile odoriferous substances by bacterial enzymes on the skin surface. The dominant armpits microbiota included four groups of bacteria such as Staphylococcus spp., Micrococcus spp., Corynebacterium spp., and Propionibacterium spp., and also fungi or yeasts. Two formulas of the roll-on deodorant products, RDEOF-1 and RDEOF-2 were developed. RDEOF-2 contained an ethanolic extract of Rhinacanthus nasutus (L.) Kurz leaves inhibited several microorganisms, including Gram-positive and Gram-negative bacteria. Sixteen healthy volunteers showed satisfactory assessment for RDEOF-2 that was greater than RDEOF-1. Armpit bacteria were collected by swab method and armpit odor was evaluated by ASTM method. Results showed dominant bacteria of two genera including Staphylococcus spp. and Corynebacterium spp. in all swab samples that mainly cause armpit odor. Armpit bacterial numbers before using deodorants were high in the range of 2x103 to 9x105 (CFU/mL). After applying RDEOF-1 and RDEOF-2, bacterial numbers decreased in the range of 1x103 to 8x105 and 3x102 to 4x105 (CFU/mL), respectively. Armpit bacteria were found in males more than in females. Deodorant products containing R. nasutus extract have been shown to reduce the bacteria that cause armpit odor. Therefore, the development of the deodorant product with natural plant extracts is warranted. © 2022, Research and Development Institute Suan Dusit University. All rights reserved.
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    Antimicrobial Effect of Deodorant Products Containing Rhinacanthus nasutus Extract for Reducing Armpit Odor
    (Graphicsite, 2023-09-26) Nartlada Onvimol; Rittipun Rungruang; Surapha Modsuwan; Tasanee Panichakul
    The aim of this study was to investigate the effectiveness of developing a roll-on deodorant that can reduce armpit microbiota bacteria to decrease armpit odor. The richest apocrine sweat glands in the armpit region secrete a variety of odor precursors that are transformed into volatile odoriferous substances by bacterial enzymes on the skin surface. The dominant armpits microbiota included four groups of bacteria such as Staphylococcus spp., Micrococcus spp., Corynebacterium spp., and Propionibacterium spp., and also fungi or yeasts. Two formulas of the roll-on deodorant products, RDEOF-1 and RDEOF-2 were developed. RDEOF-2 contained an ethanolic extract of Rhinacanthus nasutus (L.) Kurz leaves inhibited several microorganisms, including Gram-positive and Gram-negative bacteria. Sixteen healthy volunteers showed satisfactory assessment for RDEOF-2 that was greater than RDEOF-1. Armpit bacteria were collected by swab method and armpit odor was evaluated by ASTM method. Results showed dominant bacteria of two genera including Staphylococcus spp. and Corynebacterium spp. in all swab samples that mainly cause armpit odor. Armpit bacterial numbers before using deodorants were high in the range of 2x103 to 9x105 (CFU/mL). After applying RDEOF-1 and RDEOF-2, bacterial numbers decreased in the range of 1x103 to 8x105 and 3x102 to 4x105 (CFU/mL), respectively. Armpit bacteria were found in males more than in females. Deodorant products containing R. nasutus extract have been shown to reduce the bacteria that cause armpit odor. Therefore, the development of the deodorant product with natural plant extracts is warranted.
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    Antioxidant and anti-aging enzyme activities of bioactive compounds isolated from selected Zingiberaceae plants
    (Kasetsart University, 2021) Rittipun Rungruang; Wittawat Ratanathavorn; Nattaporn Boohuad; Orrapun Selamassakul; Nattapon Kaisangsri; R. Rungruang; Department of Cosmetic Science, Faculty of Science and Technology, Suan Dusit University, Bangkok, 10700, Thailand; email: rittipun_run@dusit.ac.th
    Bioactive compounds and antioxidant and enzyme inhibition activity levels were evaluated for three plants in the Zingiberaceae family in Thailand. Quantification of the phenolic and curcumin contents revealed that the largest component of all extracts was phenolic compounds (213.16-317.64 mg/g extract). The major curcuminoids in Curcuma longa L., Curcuma aromatica Salisb. and Zingiber montanum (J.Koenig) Link ex A.Dietr. were bisdemethoxycurcumin (BDMC; 27.31 mg/g extract), curcumin (16.59 mg/g extract), and curcumin (0.12 mg/g extract), respectively. The extracts from C. longa, C. aromatica and Z. montanum all showed antioxidant activity that was assessed using 1,1-diphenyl-2-picrylhydrazyl radical 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl (DPPH¥) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS¥+) radical scavenging assay. In addition, all extracts had copper chelating activity of more than 50%, with the extract from C. longa having the highest chelating activity (76.45%). All extracts from the three plants inhibited tyrosinase and elastase activity with half maximal inhibitory concentration values of 290.33-1,373.68 µg/mL and 69.61-3386.23 µg/mL, respectively. In addition, collagenase inhibition activity was observed in all extracts. The findings from this study showed that extracts from C. longa, C. aromatica and Z. montanum have potential antioxidant activity and can act as anti-tyrosinase, anti-collagenase and anti-elastase agents. The extracts could be further applied in cosmeceuticals as an active ingredient for anti-aging products. © 2021 Elsevier B.V.. All rights reserved.
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    Effects of extraction methods on the flavonoid and phenolic contents and anti-aging properties of Rhyncholaeliocattleya Haw Yuan Beauty extracts
    (Science Society of Thailand under Royal Patronage, 2021) Rittipun Rungruang; Tasanee Panichakul; Wittawat Rattanathavorn; Nattapon Kaisangsri; Orapin Kerdchoechuen; Natta Laohakunjit; Louis Kuoping Chao; R. Rungruang; Department of Cosmetic Science, Faculty of Science and Technology, Suan Dusit University, Bangkok, 10700, Thailand; email: rittipun_run@dusit.ac.th
    Rhyncholaeliocattleya Haw Yuan Beauty (Rlc. Haw Yuan Beauty) is a traditional orchid in tropical and subtropical Asian regions, which has attracted attention for its fragrant flowers with diverse colors. This study aimed to investigate the effects of different extraction methods on the phenolic profile, total flavonoid and cyanidin-3-O-glucoside contents, and anti-aging properties of Rlc. Haw Yuan Beauty crude extracts. The crude extracts were obtained using solvent, ultrasonic, and supercritical carbon dioxide fluid extraction methods. The major phenolic compounds including gallic, chlorogenic, and caffeic acids were present in all the extracts. The extract obtained with solvent extraction showed the highest total flavonoid content, whereas that obtained with supercritical fluid extraction yielded the highest cyanidin-3-O-glucoside content. Additionally, the different extraction methods had a significant influence on the antioxidant, anti-elastase, and anti-collagenase activities (p < 0.05) of Rlc. Haw Yuan Beauty extracts. The crude extracts derived from the ultrasonic and supercritical carbon dioxide fluid extraction methods were not cytotoxic to human skin fibroblasts at any of the tested concentrations (31Ð500 µg/ml). Thus, the crude extracts from Rlc. Haw Yuan Beauty could be a safe and active ingredient in the production of skincare products in the cosmetic industry for human use. © 2021 Science Society of Thailand under Royal Patronage. All rights reserved.
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    Formation of Rice Bran Glycosphingolipids Microemulsion Powders with Vitamin B1, B2, B12, and Folate as Additives for Elderly Food
    (Research and Development Institute Suan Dusit University, 2023) Junjarus Sermsathanaswadi; Siwawit Buasuwan; Dusit Angthararuk; Rittipun Rungruang; J. Sermsathanaswadi; Faculty of Science and Technology, Suan Dusit University, Bangkok, 10300, Thailand; email: janjaras_ser@dusit.ac.th
    This research investigated the extraction of glycosphingolipids from rice bran as an emulsifier to form microemulsions with vitamins B1, B2, B12 and folate being added to prepare the emulsion powder as the dietary supplement materials. Rice bran, a low-value agricultural material, was used as raw material for glycosphingolipids extraction using a solvent mixed between dichloromethane and methanol at a ratio of 2:1. The crude extract was observed in physical characteristics. The sphingosine in crude extract was analyzed by spectrophotometer technique. The emulsion was prepared using a mixture of crude extract, Polysorbate 80 and coconut oil. The average particle size of the emulsion was determined using Particle Size Analyzer. The emulsion was made into powder using various drying methods including hot air drying, spray drying and freeze-drying method. The mannan-oligosaccharides were used as an additive to replace the expensive mannitol or the energy-producing sucrose. The distribution property in distilled water of the emulsion powder was determined. The morphology and the surface of the powder emulsion were measured using SEM. The resistance to the imitation of the digestive system and encapsulation efficiency were determined. After extraction, the yield of crude glycosphingolipids extract from rice bran was 20.65%. The crude extract was a clear liquid, slightly yellow, insoluble in water and looks like oil. We found that the crude extract contained sphingosine 22.75 _g/g of crude extract from rice bran. When the emulsion was prepared, the characteristic of the emulsion showed the colloidal solution with a milky white color. The size of the emulsion without vitamins and with vitamins B1, B2, B12, and folate (total 100 ppm) were 70-75 nm and 74.4-78.1 nm, respectively. This result illustrated that the emulsion was classified as glycosphingolipid microemulsion. The emulsion powder was prepared and we found that the hot air drying and spray drying methods showed a viscous liquid with an oily smell. Whereas the freeze-drying method created the form of a light yellow, odorless and fine emulsion powder. The average particle size of emulsion was 80-100 nm. The solubility test showed that the emulsion powder was able to dissolve up to 300 g/L and 450 g/L at 25¡C and 70¡C, respectively. The morphological analysis showed that the powder emulsion was quite spherical with a diameter of less than 100 micrometers. In a simulated digestive system test, it was found that glycosphingolipid emulsion releases all vitamins in gastric simulated fluids and simulated small intestinal fluid conditions with values greater than 80%. © 2023, Research and Development Institute Suan Dusit University. All rights reserved.
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    Formation of Rice Bran Glycosphingolipids Microemulsion Powders with Vitamin B1, B2, B12, and Folate as Additives for Elderly Food
    (Graphicsite, 2024-12-18) Junjarus Sermsathanaswadi; Siwawit Buasuwan; Dusit Angthararuk; Rittipun Rungruang
    This research investigated the extraction of glycosphingolipids from rice bran as an emulsifier to form microemulsions with vitamins B1, B2, B12 and folate being added to prepare the emulsion powder as the dietary supplement materials. Rice bran, a low-value agricultural material, was used as raw material for glycosphingolipids extraction using a solvent mixed between dichloromethane and methanol at a ratio of 2:1. The crude extract was observed in physical characteristics. The sphingosine in crude extract was analyzed by spectrophotometer technique. The emulsion was prepared using a mixture of crude extract, Polysorbate 80 and coconut oil. The average particle size of the emulsion was determined using Particle Size Analyzer. The emulsion was made into powder using various drying methods including hot air drying, spray drying and freeze-drying method. The mannan-oligosaccharides were used as an additive to replace the expensive mannitol or the energy-producing sucrose. The distribution property in distilled water of the emulsion powder was determined. The morphology and the surface of the powder emulsion were measured using SEM. The resistance to the imitation of the digestive system and encapsulation efficiency were determined. After extraction, the yield of crude glycosphingolipids extract from rice bran was 20.65%. The crude extract was a clear liquid, slightly yellow, insoluble in water and looks like oil. We found that the crude extract contained sphingosine 22.75 micrograms/gram of crude extract from rice bran. When the emulsion was prepared, the characteristic of the emulsion showed the colloidal solution with a milky white color. The size of the emulsion without vitamins and with vitamins B1, B2, B12, and folate (total 100 ppm) were 70-75 nm and 74.4 -78.1 nm, respectively. This result illustrated that the emulsion was classified as glycosphingolipid microemulsion. The emulsion powder was prepared and we found that the hot air drying and spray drying methods showed a viscous liquid with an oily smell. Whereas the freeze-drying method created the form of a light yellow, odorless and fine emulsion powder. The average particle size of emulsion was 80-100 nm. The solubility test showed that the emulsion powder was able to dissolve up to 300 g/L and 450 g/L at 25°C and 70°C, respectively. The morphological analysis showed that the powder emulsion was quite spherical with a diameter of less than 100 micrometers. In a simulated digestive system test, it was found that glycosphingolipid emulsion releases all vitamins in gastric simulated fluids and simulated small intestinal fluid conditions with values greater than 80%.
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    Phytochemical screening, quantitative analysis of cyanidin-3-O-glucoside content, and anticancer activity of novel rice bran (Tubtim Chumphae rice)
    (Wolters Kluwer Medknow Publications, 2022) Rittipun Rungruang; Napassorn Peasura; Nattapon Kaisangsri; R. Rungruang; Department of Cosmetic Science, Faculty of Science and Technology, Suan Dusit University, Bangkok, 295 Nakhon Ratchasima Road, Dusit District, 10300, Thailand; email: rittipun_run@dusit.ac.th
    The objective of this research was to explore the phytochemicals in Tubtim Chumphae rice bran and determine their potential antioxidant and anticancer activities. The rice bran extract contained total phenolics, flavonoids, and anthocyanins as the major compounds. In addition, it exhibited the highest DPPH¥, followed by ABTS¥+, and FRAP radical scavenging activity. Moreover, the cytotoxic effect of the extract on Hep G2, SW620, KATO-III, BT474, and ChaGo-K-1 cancer cell lines was investigated; the obtained IC50 values were 144.6 ± 0.39, 116.9 ± 0.68, 99.6 ± 0.10, 94.4 ± 0.33, and 25.6 ± 0.45 _g/mL, respectively. Overall, the abovementioned results suggest that rice bran extract has potent anticancer activity owing to the induction of cell death in cancer cell lines. © 2022 Journal of Advanced Pharmaceutical Technology & Research.