Browsing by Author "Nongnuch Rungsawang"

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    Influence of Various Factors on Hydrolytic Enzyme Activity and COD Removal Efficiency in Cassava Starch Wastewater by Marine Actinomycete Streptomyces sp. A1-3
    (Research and Development Institute Suan Dusit University, 2024) Maliwan Kutako; Pattana Sillapachai; Yutthaya Yuyen; Paveena Tapaneeyaworawong; Nisa Siranonthana; Nongnuch Rungsawang; Janjarus Watanachote; J. Watanachote; Institute of Marine Science, Burapha University, Chonburi, 20131, Thailand; email: janjarus@buu.ac.th
    Wastewater from the production line of cassava starch factories contains a significant amount of organic matter, necessitating treatment before discharging or reusing in the factory. Actinomycetes can produce various hydrolytic enzymes, especially carbohydrate-degrading enzymes, which are important in the biological wastewater treatment process because actinomycetes can utilize several growth substances such as sugar, polysaccharides and protein. In this research, the effects of carbon source, pH, salinity and incubation time on hydrolytic enzyme activity for producing reducing sugar of a marine actinomycete Streptomyces sp. A1-3 were evaluated. The maximum hydrolytic enzyme activity was found in the oatmeal yeast extract carboxymethyl cellulose (OYC) medium with a pH value of 4.0-6.2 and a cultivation time of 4 days. Salinities in the range of 0-35 practical salinity units (psu) had no effect on the hydrolytic enzyme activity of strain A1-3. Subsequently, the efficiency of Streptomyces sp. A1-3 in reducing the chemical oxygen demand (COD) in cassava starch wastewater was evaluated using a Completely Randomized Design (CRD). The control tank had no Streptomyces sp. A1-3, while in the treatment tank, strain A1-3 was added. Aeration was supplied throughout the 16-day experiment. Water samples were collected daily for COD analysis and to measure reducing sugar levels. Within the first 4 days, the efficiency of COD reduction by strain A1-3 was 50%, higher than that in the control tank. During days 7-9, the COD reduction value of strain A1-3 remained almost stable in the range of 58-63%. Adding NaCl to adjust the salinity to 17 psu on day 10 increased the COD reduction. Between days 12 and 15 of the experiment, the COD was reduced by 79-81.82%, significantly different from the control tank. However, at the end of the experiment, the COD value in both the control and treatment tanks was higher than the standard of the Ministry of Natural Resources and Environment (Thailand), indicating the need for further treatment of these wastewaters. © 2024, Research and Development Institute Suan Dusit University. All rights reserved.
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    Influence of Various Factors on Hydrolytic Enzyme Activity and COD Removal Efficiency in Cassava Starch Wastewater by Marine Actinomycete Streptomyces sp. A1-3
    (Graphicsite, 2024-04-30) Maliwan Kutako; Pattana Sillapachai; Yutthaya Yuyen; Paveena Tapaneeyaworawong; Nisa Siranonthana; Nongnuch Rungsawang; Janjarus Watanachote
    Wastewater from the production line of cassava starch factories contains a significant amount of organic matter, necessitating treatment before discharging or reusing in the factory. Actinomycetes can produce various hydrolytic enzymes, especially carbohydrate-degrading enzymes, which are important in the biological wastewater treatment process because actinomycetes can utilize several growth substances such as sugar, polysaccharides and protein. In this research, the effects of carbon source, pH, salinity and incubation time on hydrolytic enzyme activity for producing reducing sugar of a marine actinomycete Streptomyces sp. A1-3 were evaluated. The maximum hydrolytic enzyme activity was found in the oatmeal yeast extract carboxymethyl cellulose (OYC) medium with a pH value of 4.0-6.2 and a cultivation time of 4 days. Salinities in the range of 0-35 practical salinity units (psu) had no effect on the hydrolytic enzyme activity of strain A1-3. Subsequently, the efficiency of Streptomyces sp. A1-3 in reducing the chemical oxygen demand (COD) in cassava starch wastewater was evaluated using a Completely Randomized Design (CRD). The control tank had no Streptomyces sp. A1-3, while in the treatment tank, strain A1-3 was added. Aeration was supplied throughout the 16-day experiment. Water samples were collected daily for COD analysis and to measure reducing sugar levels. Within the first 4 days, the efficiency of COD reduction by strain A1-3 was 50%, higher than that in the control tank. During days 7-9, the COD reduction value of strain A1-3 remained almost stable in the range of 58-63%. Adding NaCl
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    Lipid Production of Marine Green Microalgae Chlorella protothecoides BUUC1601 by Using Spent Coffee Grounds Hydrolysate
    (Research and Development Institute Suan Dusit University, 2023) Rachanimuk Hiransuchalert; Nisa Siranonthana; Nuttapon Chedtaisong; Pakawan Setthamongkol; Yutthaya Yuyen; Janjarus Watanachote; Paveena Tapaneeyaworawong; Nongnuch Rungsawang; Maliwan Kutako; M. Kutako; Faculty of Marine Technology, Burapha University, Chanthaburi, Chanthaburi Campus, 22170, Thailand; email: maliwan@buu.ac.th
    Spent coffee grounds are an organic waste that can be used as a source of microbial organic carbon. In this research, coffee grounds were hydrolyzed into a solution called spent coffee grounds hydrolysate (SCGH) using concentrated sulfuric acid. Then, the marine green microalgae, C. protothecoides BUUC1601, was cultured using SCGH. Growth performance and lipid accumulation of the microalgae were evaluated. The microalgae were cultured using a standard F/2 medium without and with SCGH added in the range of 2.5-15% of culture media (v/v). It was found that the microalgae had similar growth performance and biomass yield, i.e., the specific growth rate was in the range of 0.87-1.12 day-1 and the biomass yield was in the range of 0.05-0.08 g DW/L/day. Microalgae cultivation using F/2 with SCGH had an effect on lipid accumulation. It was found that using SCGH at 15% of the total volume resulted in an increase in the amount of lipid accumulation up to 66.03% of the dry weight. Of these, it was 2.89 times higher than the lipid content of microalgae cultured with no SCGH added. The content of monounsaturated fatty acids ranged from 46.15% to 46.53% and polyunsaturated fatty acids ranged from 32.40% to 34.62% of the total fatty acid content. Oleic acid (C18:1n9), an omega-9 fatty acid, was found to be the most abundant, accounting for over 30% of the total fatty acid content. In contrast, the omega-6 fatty acids linoleic acid (C18:2n6) and gamma-linolenic acid (C18:3n6) were found to be lower, accounting for 25.99%-27.20% and 6.03%-7.01% of the total fatty acid content, respectively. The omega-3 fatty acid such as alpha-linolenic acid (C18:3n3) was found at 4.20% in microalgae cultured using standard F/2 medium without SCGH, which was higher than in microalgae cultured with the addition of SCGH. Therefore, the addition of SCGH at a concentration of 2.5%-15% (v/v) can be used to cultivate C. protothecoides BUUC1601 for the lipid production with high unsaturated fatty acid content, which has the potential to be used in both aquaculture and functional food supplementation. © 2023, Research and Development Institute Suan Dusit University. All rights reserved.
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    Lipid Production of Marine Green Microalgae Chlorella protothecoides BUUC1601 by Using Spent Coffee Grounds Hydrolysate
    (Graphicsite, 2023-09-26) Rachanimuk Hiransuchalert; Nisa Siranonthana; Nuttapon Chedtaisong; Pakawan Setthamongkol; Yutthaya Yuyen; Janjarus Watanachote; Paveena Tapaneeyaworawong; Nongnuch Rungsawang; Maliwan Kutako
    Spent coffee grounds are an organic waste that can be used as a source of microbial organic carbon. In this research, coffee grounds were hydrolyzed into a solution called spent coffee grounds hydrolysate (SCGH) using concentrated sulfuric acid. Then, the marine green microalgae, C. protothecoides BUUC1601, was cultured using SCGH. Growth performance and lipid accumulation of the microalgae were evaluated. The microalgae were cultured using a standard F/2 medium without and with SCGH added in the range of 2.5-15% of culture media (v/v). It was found that the microalgae had similar growth performance and biomass yield, i.e., the specific growth rate was in the range of 0.87-1.12 day-1 and the biomass yield was in the range of 0.05-0.08 g DW/L/day. Microalgae cultivation using F/2 with SCGH had an effect on lipid accumulation. It was found that using SCGH at 15% of the total volume resulted in an increase in the amount of lipid accumulation up to 66.03% of the dry weight. Of these, it was 2.89 times higher than the lipid content of microalgae cultured with no SCGH added. The content of monounsaturated fatty acids ranged from 46.15% to 46.53% and polyunsaturated fatty acids ranged from 32.40% to 34.62% of the total fatty acid content. Oleic acid (C18:1n9), an omega-9 fatty acid, was found to be the most abundant, accounting for over 30% of the total fatty acid content. In contrast, the omega-6 fatty acids linoleic acid (C18:2n6) and gamma-linolenic acid (C18:3n6) were found to be lower, accounting for 25.99%-27.20% and 6.03%-7.01% of the total fatty acid content, respectively. The omega-3 fatty acid such as alpha-linolenic acid (C18:3n3) was found at 4.20% in microalgae cultured using standard F/2 medium without SCGH, which was higher than in microalgae cultured with the addition of SCGH. Therefore, the addition of SCGH at a concentration of 2.5%-15% (v/v) can be used to cultivate C. protothecoides BUUC1601 for the lipid production with high unsaturated fatty acid content, which has the potential to be used in both aquaculture and functional food supplementation.