Browsing by Author "Nattaporn Boohuad"

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    Additive effect of a combination of artocarpus lakoocha and glycyrrhiza glabra extracts on tyrosinase inhibition in melanoma B16 cells
    (MDPI AG, 2020) Tasanee Panichakul; Teerapat Rodboon; Prasit Suwannalert; Chanchai Tripetch; Rittipun Rungruang; Nattaporn Boohuad; Piyawan Youdee; T. Panichakul; Department of Cosmetic Science, Faculty of Science and Technology, Suan Dusit University, Bangkok, 228-228/1-3 Sirindhorn Rd. Bangplad, 10700, Thailand; email: tasanee_pan@dusit.ac.th
    Artocarpus lakoocha (Al) and Glycyrrhiza glabra (Gg) extracts have been reported to show tyrosinase inhibitory activity and melanin pigment reduction. This is the first study to assess the combination of Al and Gg extracts in enhancing inhibition of tyrosinase and reduction of melanin pigments. Al and Gg extracted by maceration in 70% and 95% ethanol were analyzed for oxyresveratrol and glabridin using Ultra High Performance Liquid Chromatography. Extracts of Al and Gg singly and combinations of Al95 and Gg95 were tested for cytotoxicity, tyrosinase inhibitory activity, and reduction of melanin pigments in melanoma B16 cells. Al95 had higher antioxidant, tyrosinase inhibitory activity and reduced more melanin pigments in B16 cells compared to Al 70, and exhibited higher levels of oxyresveratrol. Gg95 inhibited oxidative stress and mushroom tyrosinase better than Gg70, and exhibited higher levels of glabridin. Combinations of Al95 and Gg95 at various ratios (concentration of 0.1 mg/mL) were not cytotoxic to B16 cells. Interestingly, Al95 and Gg95 combined at a ratio 9:1 reduced melanin pigment up to 53% in B16 cells. This combination of Al95 and Gg95 extracts exhibited the additive effect of reducing melanin pigments by suppressing the expression of microphthalmia-associated transcription factor (MITF), tyrosinase (TYR) and tyrosinase-related protein-2 (TRP-2) in B16 cells. The combination of Al and Gg extracts could be developed as skin care products for hyperpigmentation treatment. © 2020 by the authors.
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    Antioxidant and anti-aging enzyme activities of bioactive compounds isolated from selected Zingiberaceae plants
    (Kasetsart University, 2021) Rittipun Rungruang; Wittawat Ratanathavorn; Nattaporn Boohuad; Orrapun Selamassakul; Nattapon Kaisangsri; R. Rungruang; Department of Cosmetic Science, Faculty of Science and Technology, Suan Dusit University, Bangkok, 10700, Thailand; email: rittipun_run@dusit.ac.th
    Bioactive compounds and antioxidant and enzyme inhibition activity levels were evaluated for three plants in the Zingiberaceae family in Thailand. Quantification of the phenolic and curcumin contents revealed that the largest component of all extracts was phenolic compounds (213.16-317.64 mg/g extract). The major curcuminoids in Curcuma longa L., Curcuma aromatica Salisb. and Zingiber montanum (J.Koenig) Link ex A.Dietr. were bisdemethoxycurcumin (BDMC; 27.31 mg/g extract), curcumin (16.59 mg/g extract), and curcumin (0.12 mg/g extract), respectively. The extracts from C. longa, C. aromatica and Z. montanum all showed antioxidant activity that was assessed using 1,1-diphenyl-2-picrylhydrazyl radical 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl (DPPH¥) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS¥+) radical scavenging assay. In addition, all extracts had copper chelating activity of more than 50%, with the extract from C. longa having the highest chelating activity (76.45%). All extracts from the three plants inhibited tyrosinase and elastase activity with half maximal inhibitory concentration values of 290.33-1,373.68 µg/mL and 69.61-3386.23 µg/mL, respectively. In addition, collagenase inhibition activity was observed in all extracts. The findings from this study showed that extracts from C. longa, C. aromatica and Z. montanum have potential antioxidant activity and can act as anti-tyrosinase, anti-collagenase and anti-elastase agents. The extracts could be further applied in cosmeceuticals as an active ingredient for anti-aging products. © 2021 Elsevier B.V.. All rights reserved.
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    Evaluation of Antimicrobial Activity of Rhinacanthus nasutus (L.) Kurz and Acanthus ilicifolius L. Extracts
    (Research and Development Institute Suan Dusit University, 2021) Nartlada Onvimol; Napa Onvimala; Nattaporn Boohuad; N. Onvimol; Faculty of Science and Technology, Suan Dusit University, Bangkok, 10700, Thailand; email: nartlada_onv@dusit.ac.th
    The aim of this research was to evaluate the antimicrobial properties of medical plants Rhinacanthus nasutus (L.) Kurz and Acanthus ilicifolius L. that were extracted with water/aqueous (AqER, AqEA) and ethanol (EtER, EtEA). The extracts were tested for activity and evaluated based on the effectiveness against three strains of microorganism: Gram-positive bacteria such as Staphylococcus aureus, Gram-negative bacteria such as Escherichia coli, and fungal such as Candida albicans by using the agar well diffusion and broth dilution method. The extracts from Rhinacanthus nasutus and Acanthus ilicifolius with ethanol showed the effect of inhibiting all microbes. The most effective against Candida albicans with the similar MIC and MFC values of 18.75 and 37.50 mg/mL. Meanwhile, extracts with water of Rhinacanthus nasutus and Acanthus ilicifolius with MIC values of 37.50 and 75 mg/mL and MFC values of 75 and 150 mg/mL, respectively. Conversely, these extracts showed no effect to inhibit Escherichia coli. This could be due to the capabilities of the solvents extractive and using part of the plant. Likewise, a combination of the extracts with ethanol of R. nasutus and A. ilicifolius to evaluate the efficacy of synergistic herbs can be considered from the MIC value. The antimicrobial synergy was evaluated in terms of FIC obtained from multiple-combination bactericidal/fungicidal assays. FICi value was interpreted as synergy only in ethanol extract R. nasutus+A. ilicifolius (EtRA) of 0.26. © 2021, Research and Development Institute Suan Dusit University. All rights reserved.
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    Evaluation of Antimicrobial Activity of Rhinacanthus nasutus (L.) Kurz and Acanthus ilicifolius L. Extracts
    (Graphicsite, 2023-09-26) Nartlada Onvimol; Napa Onvimala; Nattaporn Boohuad
    The aim of this research was to evaluate the antimicrobial properties of medical plants Rhinacanthus nasutus (L.) Kurz and Acanthus ilicifolius L. that were extracted with water/aqueous (AqER, AqEA) and ethanol (EtER, EtEA). The extracts were tested for activity and evaluated based on the effectiveness against three strains of microorganism: Gram-positive bacteria such as Staphylococcus aureus, Gram-negative bacteria such as Escherichia coli, and fungal such as Candida albicans by using the agar well diffusion and broth dilution method. The extracts from Rhinacanthus nasutus and Acanthus ilicifolius with ethanol showed the effect of inhibiting all microbes. The most effective against Candida albicans with the similar MIC and MFC values of 18.75 and 37.50 mg/mL. Meanwhile, extracts with water of Rhinacanthus nasutus and Acanthus ilicifolius with MIC values of 37.50 and 75 mg/mL and MFC values of 75 and 150 mg/mL, respectively. Conversely, these extracts showed no effect to inhibit Escherichia coli. This could be due to the capabilities of the solvents extractive and using part of the plant. Likewise, a combination of the extracts with ethanol of R. nasutus and A. ilicifolius to evaluate the efficacy of synergistic herbs can be considered from the MIC value. The antimicrobial synergy was evaluated in terms of FIC obtained from multiple-combination bactericidal/fungicidal assays. FICi value was interpreted as synergy only in ethanol extract R. nasutus+A. ilicifolius (EtRA) of 0.26.
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    Liposomes Encapsulating Artocarpus lakoocha Roxb. and Glycyrrhiza glabra L. Extracts: Characterization and Shelf Life of Freeze-Dried Vesicles
    (Research and Development Institute Suan Dusit University, 2020) Tasanee Panichakul; Piyawan Youdee; Nattaporn Boohuad; Khwunjit Itsarasook; Prasit Suwannalert; T. Panichakul; Department of Cosmetic Science, Faculty of Science and Technology, Suan Dusit University, Bangkok, 10300, Thailand; email: tasanee_p@yahoo.com
    Liposome is the one way of encapsulation of extracts for reducing the extract degradation. This study was to prepare the liposome entrapped extracts of Artocarpus lakoocha Roxb. (L-Al), Glycyrrhiza glabra L. (L-Gg) alone and in combination of A. lakoocha and G. glabra extracts (L-AlGg). The liposomes were prepared by Mechanochemical method and freeze-drying. For stability of liposomes, storage at 4, 25 and 45¡C for 8 weeks was performed. The trapping efficiency of liposomes and tyrosinase inhibitory activities of extracts entrapped in liposome were investigated. Results showed liposome morphology was the spherical vesicles evaluated by TEM. Before freeze-drying, liposomes had particle sizes of 156.966 ± 0.808, 140.8 ± 0.818 and 158.633 ± 4.193 nm for L-Al, L-Gg and L-AlGg, respectively. The entrapment efficiency of L-Al, L-Gg and L-AlGg was found to be 95.83 ± 13.48, 97.99 ± 5.23 and 93.90 ± 16.28 %, respectively. The tyrosinase inhibitory activities of released extracts from L-Al, L-Gg and L-AlGg were 81.57 ± 1.22, 68.92 ± 1.23 and 81.40 ± 0.64 %, respectively. After freeze-drying, the particle sizes of L-Al and L-AlGg were no significant changes, while L-Gg particle size was bigger (p < 0.01). The liposome entrapment and tyrosinase inhibitory activity of released extracts were not significantly changed after freeze-drying. This indicates good stability and no extract leakage of liposomes. In storage at 4¡C for 8 weeks, the entrapment efficiency of L-Al, L-Gg, L-AlGg and tyrosinase inhibitory activity of released extracts were not significantly different, comparing with controls. When increasing temperature of storage effected on the significantly reduction of the entrapment of liposomes and the tyrosinase inhibitory activity of released extracts (p < 0.01). Therefore, the freeze-dried liposome and storage at low temperature is recommended for stabilizing liposome and extract quality. © 2020, Research and Development Institute Suan Dusit University. All rights reserved.
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    Liposomes Encapsulating Artocarpus lakoocha Roxb. and Glycyrrhiza glabra L. Extracts: Characterization and Shelf Life of Freeze-Dried Vesicles
    (Graphicsite, 2023-09-26) Tasanee Panichakul; Piyawan Youdee; Nattaporn Boohuad; Khwunjit Itsarasook; Prasit Suwannalert
    Liposome is the one way of encapsulation of extracts for reducing the extract degradation. This study was to prepare the liposome entrapped extracts of Artocarpus lakoocha Roxb. (L-Al), Glycyrrhiza glabra L. (L-Gg) alone and in combination of A. lakoocha and G. glabra extracts (L-AlGg). The liposomes were prepared by Mechanochemical method and freeze-drying. For stability of liposomes, storage at 4, 25 and 45°C for 8 weeks was performed. The trapping efficiency of liposomes and tyrosinase inhibitory activities of extracts entrapped in liposome were investigated. Results showed liposome morphology was the spherical vesicles evaluated by TEM. Before freeze-drying, liposomes had particle sizes of 156.966 ± 0.808, 140.8 ± 0.818 and 158.633 ± 4.193 nm for L-Al, L-Gg and L-AlGg, respectively. The entrapment efficiency of L-Al, L-Gg and L-AlGg was found to be 95.83 ± 13.48, 97.99 ± 5.23 and 93.90 ± 16.28 %, respectively. The tyrosinase inhibitory activities of released extracts from L-Al, L-Gg and L-AlGg were 81.57 ± 1.22, 68.92 ± 1.23 and 81.40 ± 0.64 %, respectively. After freeze-drying, the particle sizes of L-Al and L-AlGg were no significant changes, while L-Gg particle size was bigger (p < 0.01). The liposome entrapment and tyrosinase inhibitory activity of released extracts were not significantly changed after freezedrying. This indicates good stability and no extract leakage of liposomes. In storage at 4°C for 8 weeks, the entrapment efficiency of L-Al, L-Gg, L-AlGg and tyrosinase inhibitory activity of released extracts were not significantly different, comparing with controls. When increasing temperature of storage effected on the significantly reduction of the entrapment of liposomes and the tyrosinase inhibitory activity of released extracts (p < 0.01). Therefore, the freeze-dried liposome and storage at low temperature is recommended for stabilizing liposome and extract quality.