Browsing by Author "Kriangsak Ruchusatsawat"

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    Selection of Potential Bacteria in Termite Nest and Gut for Sustainable Agriculture
    (Walailak University, 2024) Phanukit Kunhachan; Wandee Sirithana; Orapin Komutiban; Vassanasak Limkhuansuwan; Phanchai Menchai; Chanaporn Trakunjae; Thanasak Lomthong; Kriangsak Ruchusatsawat; Srisuda Samaimai; S. Samaimai; Faculty of Science and Technology, Suan Dusit University, Bangkok, 10700, Thailand; email: srisuda_sam@dusit.ac.th
    In this study, bacteria with the best abilities in cellulose degradation, siderophore production, phosphate solubility, and Pythium parasitica inhibition were selected from termite nests and guts. The isolate BTNASP 5-2, BTPK 5-3 and BTNA 5-1 from termite guts exhibited highest in siderophore production index (SPI) (4.16 ± 0.21), phosphate solubilizing index (PSI) (2.10 ± 0.14) and percentage inhibition of radial growth (PIRG) (67.07 ± 4.02 %), respectively. The BGNACMC 4-3 isolated from termite nest gave the highest cellulolytic index (CI) of 5.17 ± 0.24. Bacterial classification was performed using 16s rRNA gene sequencing. The isolates BTPK 5-3, BGNACMC 4-3 and BTNA 5-1 were found closely related to Bacillus cereus, whereas the bacterial isolate BTNASP 5-2 was closely related to Bacillus subtilis. It is also suggested that the Bacillus cereus exhibited a variety of biological activities, denoting the highest cellulase, phosphate-solubilizing and antifungal activities, while Bacillus subtilis produced only a siderophore. The results obtained suggest that the bacteria selected will be used to develop bio-compost to promote plant growth, leading to sustainable farming. © 2024, Walailak University. All rights reserved.