Browsing by Author "Kanlayaporn Chantree"

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    Antioxidant Activities and Cytotoxicity Effect on Normal Human Dermal Fibroblasts of Roselle (Hibiscus sabdariffa L.) Calyces Extracts and W/O/W Emulsion Loaded Extract for Cosmetic Applications
    (Research and Development Institute Suan Dusit University, 2023) Khwunjit Itsarasook; Piyanuch Prompamorn; Jittarawadee Tanghiranrat; Kanlayaporn Chantree; Itsaret Itsarasuk; K. Itsarasook; Faculty of Science and Technology, Suan Dusit University, Bangkok, 10700, Thailand; email: khwunjit_its@dusit.ac.th
    The objective of this study was to evaluate the antioxidant activities and cytotoxicity effect on normal human dermal fibroblasts of roselle (Hibiscus sabdariffa L.) calyx extracts and to load with the roselle extract in the inner phase of water in oil in water multiple emulsion for application in facial cosmetic. The extracts of dried roselle calyces were prepared by a maceration technique using ethanol/water at concentrations of 30%, 70%, 95% and 0% (100% DI water). Obtained results showed that the 30% ethanolic extract had a higher amount of total phenolic and anthocyanin content than other extracts. According to the DPPH and ABTS assays, the 30% ethanolic extract exhibited dose-dependent antioxidant activity higher than other extracts with an IC50 value of 0.432±0.001 mg/mL and 0.0855±0.01 mg/mL, respectively. The formulation of water in oil in water multiple emulsion containing the roselle calyx extract was prepared by a beaker method with two-step emulsification. The prepared multiple emulsion showed a light pink color with homogeneously formula. The physiochemical stability of the prepared formula was evaluated at accelerated conditions; room temperature (30±5¡C), low temperature (4±1¡C) and high temperature (40±1¡C) for 30 days and 6 cycles of freeze (-25¡C, 24 h)-thaw (4¡C, 24 h). Results found that the tested formula showed a good physicochemical stability compared to the initial condition. All finding suggests possible application for the 30% ethanolic extract of roselle calyces as a natural active ingredient in skin cosmetic products. © 2023, Research and Development Institute Suan Dusit University. All rights reserved.
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    Antioxidant Activities and Cytotoxicity Effect on Normal Human Dermal Fibroblasts of Roselle (Hibiscus sabdariffa L.) Calyces Extracts and W/O/W Emulsion Loaded Extract for Cosmetic Applications
    (Graphicsite, 2023-11-11) Khwunjit Itsarasook; Piyanuch Prompamorn; Jittarawadee Tanghiranrat; Kanlayaporn Chantree; Itsaret Itsarasuk
    The objective of this study was to evaluate the antioxidant activities and cytotoxicity effect on normal human dermal fibroblasts of roselle (Hibiscus sabdariffa L.) calyx extracts and to load with the roselle extract in the inner phase of water in oil in water multiple emulsion for application in facial cosmetic. The extracts of dried roselle calyces were prepared by a maceration technique using ethanol/water at concentrations of 30%, 70%, 95% and 0% (100% DI water). Obtained results showed that the 30% ethanolic extract had a higher amount of total phenolic and anthocyanin content than other extracts. According to the DPPH and ABTS assays, the 30% ethanolic extract exhibited dose-dependent antioxidant activity higher than other extracts with an IC50 value of 0.432±0.001 mg/mL and 0.0855±0.01 mg/mL, respectively. The formulation of water in oil in water multiple emulsion containing the roselle calyx extract was prepared by a beaker method with twostep emulsification. The prepared multiple emulsion showed a light pink color with homogeneously formula. The physiochemical stability of the prepared formula was evaluated at accelerated conditions; room temperature (30±5ºC), low temperature (4±1ºC) and high temperature (40±1ºC) for 30 days and 6 cycles of freeze (-25ºC, 24 h)-thaw (4ºC, 24 h). Results found that the tested formula showed a good physicochemical stability compared to the initial condition. All finding suggests possible application for the 30% ethanolic extract of roselle calyces as a natural active ingredient in skin cosmetic products.
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    Evaluation of Terminalia chebula Retz. Extract Against Caries-associated Bacteria as an Alternative Compound for Oral Care Products
    (Research and Development Institute Suan Dusit University, 2022) Piyanuch Prompamorn; Khwunjit Itsarasook; Jitrawadee Tanghiranrat; Chanchai Tripetch; Kanlayaporn Chantree; P. Prompamorn; Faculty of Science and Technology, Suphanburi Campus, Suan Dusit University, Suphanburi, 72000, Thailand; email: Piyanuch_pro@dusit.ac.th
    The objective of this study was to investigate the efficiency of Terminalia chebula Retz. fruit extract for further use as an active ingredient in oral care products. The quantification of major constituent, the antioxidant activity and antibacterial activity of an extract against Streptococcus mutans in both planktonic and biofilm form were measured. The results showed that ellagic acid was the major constituent of an extract and the amount of ellagic acid contained in the extract was 36.671 mg/g. The extract exhibit free radical scavenging activity with IC50 value of 0.44±0.01 mg/mL by DPPH assay. For antibacterial activity against S. mutans, T. chebula Retz. extract possess high inhibitory effect on S. mutans in both planktonic form and biofilm form. The MIC50 and MBC value of an extract against planktonic form of S. mutans were 0.47±0.28 and 6.25 mg/mL, respectively. For biofilm form of S. mutans, the extract has a high capability to preventing biofilm formation and eradicating the existing biofilm. The MBIC50 and MBEC50 of an extract against biofilm form of S. mutants were 4.47±0.32 and 9.64±0.39 mg/mL, respectively. © 2022, Research and Development Institute Suan Dusit University. All rights reserved.
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    Evaluation of Terminalia chebula Retz. Extract Against Caries-associated Bacteria as an Alternative Compound for Oral Care Products
    (Graphicsite, 2023-09-26) Piyanuch Prompamorn; Khwunjit Itsarasook; Jitrawadee Tanghiranra; Chanchai Tripetch; Kanlayaporn Chantree
    The objective of this study was to investigate the efficiency of Terminalia chebula Retz. fruit extract for further use as an active ingredient in oral care products. The quantification of major constituent, the antioxidant activity and antibacterial activity of an extract against Streptococcus mutans in both planktonic and biofilm form were measured. The results showed that ellagic acid was the major constituent of an extract and the amount of ellagic acid contained in the extract was 36.671 mg/g. The extract exhibit free radical scavenging activity with IC50 value of 0.44±0.01 mg/mL by DPPH assay. For antibacterial activity against S. mutans, T. chebula Retz. extract possess high inhibitory effect on S. mutans in both planktonic form and biofilm form. The MIC50 and MBC value of an extract against planktonic form of S. mutans were 0.47±0.28 and 6.25 mg/mL, respectively. For biofilm form of S. mutans, the extract has a high capability to preventing biofilm formation and eradicating the existing biofilm. The MBIC50 and MBEC50 of an extract against biofilm form of S. mutants were 4.47±0.32 and 9.64±0.39 mg/mL, respectively
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    Preparation, Characterization and Evaluation of Octyl Methoxycinnamate (OMC)-Loaded Solid Lipid Nanoparticles (SLNs) by Using a Microemulsion Technique
    (Research and Development Institute Suan Dusit University, 2022) Khwunjit Itsarasook; Piyanuch Prompamorn; Surapa Modsuwan; Jittarawadee Tanghiranrat; Orapin Komutiban; Woraphot Haritakun; Jantharat Wutisatwongkul; Kanlayaporn Chantree; K. Itsarasook; Faculty of Science and Technology, Suphanburi Campus, Suan Dusit University, Suphanburi, 72000, Thailand; email: khwunjit_its@dusit.ac.th
    This research prepared the solid lipid nanoparticles (SLNs) based on microemulsion to load octyl methoxycinnamate (OMC) as a UV absorber for instability protection. The optimal microemulsion formulation was selected by visual assessment according to a transparent and thermodynamically system form. The optimal microemulsion obtained from the experiment composed of 10% w/w glyceryl monostearate (GMS, solid lipid), 10% w/w Tween 80 (surfactant), 15% w/w PEG-40 hydrogenated castor oil (surfactant) and 20% w/w ethanol (co-surfactant). Various processing parameters for the preparation of SLN was carried out using three factors including, 1) warm microemulsion (mL) and iced water ratio (mL), 2) homogenization speed (rpm) and 3) homogenization time (min) and two responses assessed were particle size and polydispersity index (PDI) to obtain a SLNs batch with smaller particle size and optimum PDI. The OMC-loaded SLN had 693.07± 0.05 nm mean particle size and 0.56±0.04 PDI, prepared by optimal processing parameters. The surface morphology of the SLNs was assessed by transmission electron microscopy (TEM), the result found that OMC-loaded SLN showed a smooth surface and spherical shape. Entrapment efficiency of OMC-loaded SLN was found to be 99.89±0.020%. The stability testing of the prepared SLN was studied. Result found that OMC-loaded SLN showed slightly lower stable compared with the initial condition. All obtained results indicated that this method and processing parameters can prepare the SLNs which was suitable for cosmetic application. © 2022, Research and Development Institute Suan Dusit University. All rights reserved.
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    Preparation, Characterization and Evaluation of Octyl Methoxycinnamate (OMC)-Loaded Solid Lipid Nanoparticles (SLNs) by Using a Microemulsion Technique
    (Graphicsite, 2023-09-26) Khwunjit Itsarasook; Piyanuch Prompamorn; Surapa Modsuwan; Jittarawadee Tanghiranrat; Orapin Komutiban; Woraphot Haritakun; Jantharat Wutisatwongkul; Kanlayaporn Chantree
    This research prepared the solid lipid nanoparticles (SLNs) based on microemulsion to load octyl methoxycinnamate (OMC) as a UV absorber for instability protection. The optimal microemulsion formulation was selected by visual assessment according to a transparent and thermodynamically system form. The optimal microemulsion obtained from the experiment composed of 10% w/w glyceryl monostearate (GMS, solid lipid), 10% w/w Tween 80 (surfactant), 15% w/w PEG-40 hydrogenated castor oil (surfactant) and 20% w/w ethanol (co-surfactant). Various processing parameters for the preparation of SLN was carried out using three factors including, 1) warm microemulsion (mL) and iced water ratio (mL), 2) homogenization speed (rpm) and 3) homogenization time (min) and two responses assessed were particle size and polydispersity index (PDI) to obtain a SLNs batch with smaller particle size and optimum PDI. The OMC-loaded SLN had 693.07± 0.05 nm mean particle size and 0.56±0.04 PDI, prepared by optimal processing parameters. The surface morphology of the SLNs was assessed by transmission electron microscopy (TEM), the result found that OMC-loaded SLN showed a smooth surface and spherical shape. Entrapment efficiency of OMC-loaded SLN was found to be 99.89±0.020% . The stability testing of the prepared SLN was studied. Result found that OMC-loaded SLN showed slightly lower stable compared with the initial condition. All obtained results indicated that this method and processing parameters can prepare the SLNs which was suitable for cosmetic application.