Browsing by Author "Jarupa Viyoch"

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    Artocarpin-enriched extract reverses collagen metabolism in UV-exposed fibroblasts
    (Versita, 2014) Khwunjit Itsarasook; Kornkanok Ingkaninan; Jarupa Viyoch; J. Viyoch; Department of Pharmaceutical Technology, Naresuan University, Phitsanulok, 65000, Thailand; email: jarupav@nu.ac.th
    In previous studies, the Artocarpus incisus extract containing 45% w/w artocarpin showed activities of antioxidation, antimelanogenesis and restoration of wrinkled-skin fibroblasts. Here, extract containing 90% w/w artocarpin was tested for its antioxidant activity and in ultraviolet (UV) A-irradiated fibroblasts, its ability to restore type I collagen and inhibit matrix metalloproteinase-1 (MMP-1) elevation. This extract was a less effective antioxidant of EC50 of 116.0 ± 5.1 _g/mL than L-ascorbic acid (9.7 ± 0.01 _g/mL). The extract (0.625-50 _g/mL) showed no cytotoxicity toward primary human skin fibroblasts. MMP-1 was markedly elevated at 72 h after UVA irradiation compared to non-irradiation cells (p < 0.01). This UVA-induced elevation was inhibited by 50 _g/mL extract or 50 ng/mL all-trans retinoic acid. In an aged and sun-exposed skin tissue culture model, the increase of epidermal thickness in the 250 _g/mL artocarpin-enriched extract or 75 _g/mL all-trans retinoic acid-treated group when compared to the non-treated group was markedly observed since day 1 of treatment. Moreover, the extract or all-trans retinoic acid-treated groups exhibited higher density of immunofluorescence staining of type I collagen than non-treated group. This coincides with significantly higher (p < 0.05) collagen content, as indicated by measuring hydroxyproline. Our results firstly revealed that the artocarpin-enriched extract reversed the activities of UVA-irradiated fibroblasts and improved the type I collagen deposition in aged/photoaged skin. © 2014 Versita Warsaw and Springer-Verlag Wien.
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    Ethanol extract of Terminalia chebula fruit protects against UVB-induced skin damage
    (Taylor and Francis Ltd, 2016) Swanya Yakaew; Khwunjit Itsarasook; Jatuporn Ngoenkam; Arum Jessadayannamaetha; Jarupa Viyoch; Malyn Ungsurungsie; M. Ungsurungsie; Research & Development Division, S & J International Enterprises Public Company Limited, Bangkok, Thailand; email: malynthai@gmail.com
    Context: The fruit of Terminalia chebula Retz. (Combretaceae) has been used for several therapeutic purposes in Thai folk medicines. Currently, the ethanol extracts containing antioxidant compounds have shown the ability to promote collagen synthesis. Objective: This purpose of this work was to study the effects of the ethanol extract from T. chebula fruit on the inhibition of cutaneous photodamage. Materials and methods: The viability of human skin fibroblasts after incubation with T. chebula at concentration 0.5�50 _g/mL for 24, 48 and 72 h was assessed by using sodium 3_-[(phenyl-amino)-carbonyl]-3,4,tetrazolium-bis(4-methoxy-6-notro)benzene-sulphonic acid hydrate (XTT). The levels of type I procollagen and matrix metalloproteinases (MMP)-1 and MMP-13 produced by UVB-irradiated fibroblasts were determined by ELISA. Skin thickness and collagen content caused by long-term UVB irradiation in male ICR mice were determined from haematoxylin and eosin stained tissue sections and spectrophotometric measurement of hydroxyproline. Results: The extract (0.5�50 _g/mL) had no effect on cell viability or morphology of the human fibroblasts. In vitro studies showed that the T. chebula extract reduced the UVB-induced MMP-1 and MMP-13 expression, whereas an increased production of type I procollagen was observed. In a UVB-irradiated animal model, male ICR mice with hair shaved were chronically exposed to UVB which lead to epidermal thickness and loss of hydroxyproline. However, these effects were fully prevented by the topical application of the T. chebula ethanol extract. Discussion and conclusion: These data suggested that the T. chebula ethanol fruit extract is an efficacious pharmaceutical protectant of skin against photodamage. � 2016 Informa UK Limited, trading as Taylor & Francis Group.