Browsing by Author "Chanchai Tripetch"

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    Additive effect of a combination of artocarpus lakoocha and glycyrrhiza glabra extracts on tyrosinase inhibition in melanoma B16 cells
    (MDPI AG, 2020) Tasanee Panichakul; Teerapat Rodboon; Prasit Suwannalert; Chanchai Tripetch; Rittipun Rungruang; Nattaporn Boohuad; Piyawan Youdee; T. Panichakul; Department of Cosmetic Science, Faculty of Science and Technology, Suan Dusit University, Bangkok, 228-228/1-3 Sirindhorn Rd. Bangplad, 10700, Thailand; email: tasanee_pan@dusit.ac.th
    Artocarpus lakoocha (Al) and Glycyrrhiza glabra (Gg) extracts have been reported to show tyrosinase inhibitory activity and melanin pigment reduction. This is the first study to assess the combination of Al and Gg extracts in enhancing inhibition of tyrosinase and reduction of melanin pigments. Al and Gg extracted by maceration in 70% and 95% ethanol were analyzed for oxyresveratrol and glabridin using Ultra High Performance Liquid Chromatography. Extracts of Al and Gg singly and combinations of Al95 and Gg95 were tested for cytotoxicity, tyrosinase inhibitory activity, and reduction of melanin pigments in melanoma B16 cells. Al95 had higher antioxidant, tyrosinase inhibitory activity and reduced more melanin pigments in B16 cells compared to Al 70, and exhibited higher levels of oxyresveratrol. Gg95 inhibited oxidative stress and mushroom tyrosinase better than Gg70, and exhibited higher levels of glabridin. Combinations of Al95 and Gg95 at various ratios (concentration of 0.1 mg/mL) were not cytotoxic to B16 cells. Interestingly, Al95 and Gg95 combined at a ratio 9:1 reduced melanin pigment up to 53% in B16 cells. This combination of Al95 and Gg95 extracts exhibited the additive effect of reducing melanin pigments by suppressing the expression of microphthalmia-associated transcription factor (MITF), tyrosinase (TYR) and tyrosinase-related protein-2 (TRP-2) in B16 cells. The combination of Al and Gg extracts could be developed as skin care products for hyperpigmentation treatment. © 2020 by the authors.
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    Evaluation of Terminalia chebula Retz. Extract Against Caries-associated Bacteria as an Alternative Compound for Oral Care Products
    (Research and Development Institute Suan Dusit University, 2022) Piyanuch Prompamorn; Khwunjit Itsarasook; Jitrawadee Tanghiranrat; Chanchai Tripetch; Kanlayaporn Chantree; P. Prompamorn; Faculty of Science and Technology, Suphanburi Campus, Suan Dusit University, Suphanburi, 72000, Thailand; email: Piyanuch_pro@dusit.ac.th
    The objective of this study was to investigate the efficiency of Terminalia chebula Retz. fruit extract for further use as an active ingredient in oral care products. The quantification of major constituent, the antioxidant activity and antibacterial activity of an extract against Streptococcus mutans in both planktonic and biofilm form were measured. The results showed that ellagic acid was the major constituent of an extract and the amount of ellagic acid contained in the extract was 36.671 mg/g. The extract exhibit free radical scavenging activity with IC50 value of 0.44±0.01 mg/mL by DPPH assay. For antibacterial activity against S. mutans, T. chebula Retz. extract possess high inhibitory effect on S. mutans in both planktonic form and biofilm form. The MIC50 and MBC value of an extract against planktonic form of S. mutans were 0.47±0.28 and 6.25 mg/mL, respectively. For biofilm form of S. mutans, the extract has a high capability to preventing biofilm formation and eradicating the existing biofilm. The MBIC50 and MBEC50 of an extract against biofilm form of S. mutants were 4.47±0.32 and 9.64±0.39 mg/mL, respectively. © 2022, Research and Development Institute Suan Dusit University. All rights reserved.
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    Evaluation of Terminalia chebula Retz. Extract Against Caries-associated Bacteria as an Alternative Compound for Oral Care Products
    (Graphicsite, 2023-09-26) Piyanuch Prompamorn; Khwunjit Itsarasook; Jitrawadee Tanghiranra; Chanchai Tripetch; Kanlayaporn Chantree
    The objective of this study was to investigate the efficiency of Terminalia chebula Retz. fruit extract for further use as an active ingredient in oral care products. The quantification of major constituent, the antioxidant activity and antibacterial activity of an extract against Streptococcus mutans in both planktonic and biofilm form were measured. The results showed that ellagic acid was the major constituent of an extract and the amount of ellagic acid contained in the extract was 36.671 mg/g. The extract exhibit free radical scavenging activity with IC50 value of 0.44±0.01 mg/mL by DPPH assay. For antibacterial activity against S. mutans, T. chebula Retz. extract possess high inhibitory effect on S. mutans in both planktonic form and biofilm form. The MIC50 and MBC value of an extract against planktonic form of S. mutans were 0.47±0.28 and 6.25 mg/mL, respectively. For biofilm form of S. mutans, the extract has a high capability to preventing biofilm formation and eradicating the existing biofilm. The MBIC50 and MBEC50 of an extract against biofilm form of S. mutants were 4.47±0.32 and 9.64±0.39 mg/mL, respectively