Faculty of Science and Technology

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Browsing Faculty of Science and Technology by Author "Srisuda Samaimai"

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    Selection of Potential Bacteria in Termite Nest and Gut for Sustainable Agriculture
    (2024-07-01) Srisuda Samaimai
    Syzygiumgenus is extensively used in managing different diseases such as antimicrobial, anti-diarrheal, antidiabetic, analgesic, anti-inflammatory, antioxidant, and anticancer activities. The present study aims to investigate phytochemicals by screening tests andGC-MS analysis and evaluate the antioxidants, cytotoxicity, analgesic, and anti-diarrheal activity of the extracts of the leaves of Syzygium reticulatum. The leaves of the S. reticulatumwere extracted with hexane and methanol by the maceration process successively, denoted as SRH and SRM. The extract’s phytochemical analyses found flavonoids, alkaloids, saponins, tannins, glycosides, and carbohydrates. The GC-MS analysis of the methanol extract identified 8compounds. The major components were identified as Diisooctyl phthalate (96.903%), 2-methyltetracosane (1.094%), and others with fewer amounts. The result of the antioxidant study of the extracts as total phenolic content of SRH 8.92±0.62 and SRM 340.107±11.11mg/g, respectively, total flavonoids content of SRH 7.85±0.71 and SRM 105.16±0.93mg/g, IC50 values of the DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging assay of SRM was 14.67 μg/mL, SRH was 41.26 μg/mL and ascorbic acid 14.08 μg/mL; the total antioxidant capacity of SRH was 21.33±2.52mg/g, whereas SRM was 353.71±4.59mg/g. The results of these studies suggest that methanol extract has a more potent antioxidant capacity than hexane extract. The methanol extract also exhibited higher toxicity (LC50 value 49.99μg/mL) compared to hexane extract (LC50 value 370.348μg/mL), as revealed by the Brine shrimp nauplii cytotoxic study. An analgesic study of the methanol extract found that a lower dose (250mg/kg) has more significant analgesic activity than the higher dose and standard drug aspirin. At 90 min, the hot plate’s highest percentage of latency elongation was 110.32, while the acetic acid-induced writhing inhibition was 63.04 %. At a dose of 500 mg/kg, the methanol extract also shown good efficacy in mice suffering from castor oil and magnesium sulfate-induced diarrhea. The findings from the study of the leaves of the S. reticulatumexhibited that methanol extracts have significant antioxidant, cytotoxic, analgesic, and anti-diarrheal activity