Ubol ChuensumranTasanee PanichakulThun IngkakulPoom Adisakwattana2025-03-102025-03-102020Southeast Asian Journal of Tropical Medicine and Public Health12515622-s2.0-85114373400https://repository.dusit.ac.th//handle/123456789/4760Studies on genomic instabilities of intrahepatic cholangiocarcinoma (ICC) from Thai patients have shown 52% variations in a specific region of ubiquitin-specific protease 14 gene (USP14) located on 18p11.32. Inhibition of USP14 expression in three different primary cells cultured from resected ICC tissues by transfecting with double-strand RNA interference targeting exon 16 region of USP14 mRNA resulted in 6-50% reduction in mRNA levels, with cells with low USP14 expression being more affected. These findings lend support to a potential in developing strategies against USP14 in ICC. © 2020, SEAMEO TROPMED Network. All rights reserved.Intrahepatic cholangiocarcinomaPrimary cell cultureRNAiUbiquitin-specific protease 14ArticleScopus