Shimpei AikawaSirilak BarameeJunjarus SermsathanaswadiPhakhinee ThianhengChakrit TachaapaikoonAyumi ShikataRattiya WaeonukulPatthra PasonKhanok RatanakhanokchaiAkihiko Kosugi2025-03-102025-03-102018Systematic and Applied Microbiology723202010.1016/j.syapm.2018.01.0102-s2.0-85042360404https://repository.dusit.ac.th//handle/123456789/4921An anaerobic, cellulolytic-xylanolytic bacterium, designated strain A7, was isolated from a cellulose-degrading bacterial community inhabiting bovine manure compost on Ishigaki Island, Japan, by enrichment culture using unpretreated corn stover as the sole carbon source. The strain was Gram-positive, non-endospore forming, non-motile, and formed orange colonies on solid medium. Strain A7 was identified as Herbivorax saccincola by DNA-DNA hybridization, and phylogenetic analysis based on 16S rRNA gene sequences showed that it was closely related to H. saccincola GGR1 (= DSM 101079T). H. saccincola A7 (= JCM 31827 = DSM 104321) had quite similar phenotypic characteristics to those of strain GGR1. However, the optimum growth of A7 was at alkaline pH (9.0) and 55 �C, compared to pH 7.0 at 60 �C for GGR1, and the fatty acid profile of A7 contained 1.7-times more C17:0 iso than GGR1. The draft genome sequence revealed that H. saccincola A7 possessed a cellulosome-like extracellular macromolecular complex, which has also been found for Clostridium thermocellum and C. clariflavum. H. saccincola A7 contained more glycoside hydrolases (GHs) belonging to GH families-11 and -2, and more diversity of xylanolytic enzymes, than C. thermocellum and C. clariflavum. H. saccincola A7 could grow on xylan because it encoded essential genes for xylose metabolism, such as a xylose transporter, xylose isomerase, xylulokinase, and ribulose-phosphate 3-epimerase, which are absent from C. thermocellum. These results indicated that H. saccincola A7 has great potential as a microorganism that can effectively degrade lignocellulosic biomass. � 2018 Elsevier GmbHAll Open Access; Bronze Open AccessAlkaliphileCellulolytic bacteriumCellulosomeGenome sequenceHerbivorax saccincolaLignocelluloseArticleScopus